Determination of nuclear transcription factor activity using a modified electrophoretic mobility shift assay

Detalhes bibliográficos
Autor(a) principal: Wang,Yong
Data de Publicação: 2008
Outros Autores: Huang,Wenhua, Peng,Daizhi, Hu,Yuanbin
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400025
Resumo: In this work, several major procedures of the electrophoretic mobility shift assay (EMSA) were modified including swift extraction of the nucleic protein, labeling of the probe and radioautography. The modified assay required shorter time, simplified the nucleic protein extraction, increased the radioactivity of the labeling probe, skipped the tedious process of gel drying, and produced clear images. Its results were comparable, reproducible and stable. It thus has merited for wide application.
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spelling Determination of nuclear transcription factor activity using a modified electrophoretic mobility shift assayNuclear transcription factorelectrophoretic mobility shift assaymethodologymodificationIn this work, several major procedures of the electrophoretic mobility shift assay (EMSA) were modified including swift extraction of the nucleic protein, labeling of the probe and radioautography. The modified assay required shorter time, simplified the nucleic protein extraction, increased the radioactivity of the labeling probe, skipped the tedious process of gel drying, and produced clear images. Its results were comparable, reproducible and stable. It thus has merited for wide application.Instituto de Tecnologia do Paraná - Tecpar2008-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400025Brazilian Archives of Biology and Technology v.51 n.4 2008reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132008000400025info:eu-repo/semantics/openAccessWang,YongHuang,WenhuaPeng,DaizhiHu,Yuanbineng2008-08-26T00:00:00Zoai:scielo:S1516-89132008000400025Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2008-08-26T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Determination of nuclear transcription factor activity using a modified electrophoretic mobility shift assay
title Determination of nuclear transcription factor activity using a modified electrophoretic mobility shift assay
spellingShingle Determination of nuclear transcription factor activity using a modified electrophoretic mobility shift assay
Wang,Yong
Nuclear transcription factor
electrophoretic mobility shift assay
methodology
modification
title_short Determination of nuclear transcription factor activity using a modified electrophoretic mobility shift assay
title_full Determination of nuclear transcription factor activity using a modified electrophoretic mobility shift assay
title_fullStr Determination of nuclear transcription factor activity using a modified electrophoretic mobility shift assay
title_full_unstemmed Determination of nuclear transcription factor activity using a modified electrophoretic mobility shift assay
title_sort Determination of nuclear transcription factor activity using a modified electrophoretic mobility shift assay
author Wang,Yong
author_facet Wang,Yong
Huang,Wenhua
Peng,Daizhi
Hu,Yuanbin
author_role author
author2 Huang,Wenhua
Peng,Daizhi
Hu,Yuanbin
author2_role author
author
author
dc.contributor.author.fl_str_mv Wang,Yong
Huang,Wenhua
Peng,Daizhi
Hu,Yuanbin
dc.subject.por.fl_str_mv Nuclear transcription factor
electrophoretic mobility shift assay
methodology
modification
topic Nuclear transcription factor
electrophoretic mobility shift assay
methodology
modification
description In this work, several major procedures of the electrophoretic mobility shift assay (EMSA) were modified including swift extraction of the nucleic protein, labeling of the probe and radioautography. The modified assay required shorter time, simplified the nucleic protein extraction, increased the radioactivity of the labeling probe, skipped the tedious process of gel drying, and produced clear images. Its results were comparable, reproducible and stable. It thus has merited for wide application.
publishDate 2008
dc.date.none.fl_str_mv 2008-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400025
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400025
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132008000400025
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.51 n.4 2008
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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