The 95RGD97 sequence on the Aα chain of fibrinogen is essential for binding to its erythrocyte receptor

Detalhes bibliográficos
Autor(a) principal: Carvalho, Filomena Almeida
Data de Publicação: 2018
Outros Autores: Guedes, Ana Filipa, Duval, Cedric, Macrae, Fraser L., Swithenbank, Luke, Farrell, David H., Ariëns, Robert A. S., Santos, Nuno C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/37854
Resumo: © 2018 Carvalho et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
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spelling The 95RGD97 sequence on the Aα chain of fibrinogen is essential for binding to its erythrocyte receptorAtomic force microscopyFibrinogenFibrin clotErythrocyte aggregationMutant protein© 2018 Carvalho et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).BACKGROUND: Erythrocyte aggregation, a cardiovascular risk factor, is increased by high plasma fibrinogen levels. Here, the effect of different fibrinogen mutations on binding to its human erythrocyte receptor was assessed in order to identify the interaction sites. METHODS: Three fibrinogen variants were tested, specifically mutated in their putative integrin recognition sites on the Aα chain (mutants D97E, D574E and D97E/D574E) and compared with wild-type fibrinogen. RESULTS: Atomic force microscopy-based force spectroscopy measurements showed a significant decrease both on the fibrinogen-erythrocyte binding force and on its frequency for fibrinogen with the D97E mutation, indicating that the corresponding arginine-glycine-aspartate sequence (residues 95-97) is involved in this interaction, and supporting that the fibrinogen receptor on erythrocytes has a β3 subunit. Changes in the fibrin clot network structure obtained with the D97E mutant were observed by scanning electron microscopy. CONCLUSION: These findings may lead to innovative perspectives on the development of new therapeutic approaches to overcome the risks of fibrinogen-driven erythrocyte hyperaggregation.The authors thank Teresa Freitas (IMM, FM, UL) for excellent technical assistance. This work was supported by Fundação para a Ciência e a Tecnologia – Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal) grants PTDC/QUI-BIQ/119509/2010 and PTDC/BBB-BMD/6307/2014, FEDER, through POR Lisboa 2020 - Programa Operacional Regional de Lisboa, PORTUGAL 2020 and FCT-MCTES (LISBOA-01-0145-FEDER-007391), and by the Calouste Gulbenkian Foundation (Portugal). AFG also acknowledges FCT-MCTES PhD fellowship SFRH/BD/84414/2012.Dove Medical PressRepositório da Universidade de LisboaCarvalho, Filomena AlmeidaGuedes, Ana FilipaDuval, CedricMacrae, Fraser L.Swithenbank, LukeFarrell, David H.Ariëns, Robert A. S.Santos, Nuno C.2019-04-04T15:53:39Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/37854engInternational Journal of Nanomedicine 2018:13 1985–19921176-911410.2147/IJN.S154523info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:35:15Zoai:repositorio.ul.pt:10451/37854Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:51:49.502516Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The 95RGD97 sequence on the Aα chain of fibrinogen is essential for binding to its erythrocyte receptor
title The 95RGD97 sequence on the Aα chain of fibrinogen is essential for binding to its erythrocyte receptor
spellingShingle The 95RGD97 sequence on the Aα chain of fibrinogen is essential for binding to its erythrocyte receptor
Carvalho, Filomena Almeida
Atomic force microscopy
Fibrinogen
Fibrin clot
Erythrocyte aggregation
Mutant protein
title_short The 95RGD97 sequence on the Aα chain of fibrinogen is essential for binding to its erythrocyte receptor
title_full The 95RGD97 sequence on the Aα chain of fibrinogen is essential for binding to its erythrocyte receptor
title_fullStr The 95RGD97 sequence on the Aα chain of fibrinogen is essential for binding to its erythrocyte receptor
title_full_unstemmed The 95RGD97 sequence on the Aα chain of fibrinogen is essential for binding to its erythrocyte receptor
title_sort The 95RGD97 sequence on the Aα chain of fibrinogen is essential for binding to its erythrocyte receptor
author Carvalho, Filomena Almeida
author_facet Carvalho, Filomena Almeida
Guedes, Ana Filipa
Duval, Cedric
Macrae, Fraser L.
Swithenbank, Luke
Farrell, David H.
Ariëns, Robert A. S.
Santos, Nuno C.
author_role author
author2 Guedes, Ana Filipa
Duval, Cedric
Macrae, Fraser L.
Swithenbank, Luke
Farrell, David H.
Ariëns, Robert A. S.
Santos, Nuno C.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Carvalho, Filomena Almeida
Guedes, Ana Filipa
Duval, Cedric
Macrae, Fraser L.
Swithenbank, Luke
Farrell, David H.
Ariëns, Robert A. S.
Santos, Nuno C.
dc.subject.por.fl_str_mv Atomic force microscopy
Fibrinogen
Fibrin clot
Erythrocyte aggregation
Mutant protein
topic Atomic force microscopy
Fibrinogen
Fibrin clot
Erythrocyte aggregation
Mutant protein
description © 2018 Carvalho et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
2019-04-04T15:53:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/37854
url http://hdl.handle.net/10451/37854
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Nanomedicine 2018:13 1985–1992
1176-9114
10.2147/IJN.S154523
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Dove Medical Press
publisher.none.fl_str_mv Dove Medical Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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