Microplate reader analysis of triatomine saliva effect on erythrocyte aggregation
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Materials research (São Carlos. Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392007000100008 |
Resumo: | Our hypothesis is that the action of aggregating and disaggregating substances in the blood can be detected and quantified by the Microplate Reader. To ascertain the validity of this hypothesis, we selected two types of blood: one that naturally presents erythrocyte aggregation (pig blood) and the other that does not present aggregation (bovine blood). One important reason for the choice of pig blood is that its erythrocyte aggregation resembles that of human blood. T. infestans saliva was added to the pig blood as a disaggregating substance, while bovine fibrinogen was added to the bovine blood as a substance that promotes erythrocyte aggregation. We investigated the dynamic viscosity (h) of these mammals' blood, of T. infestans saliva and of the absorption (A) by Microplate Reader, carrying out UV-Vis spectrophotometric assays of pig plasma with different concentrations of triatominae saliva and of bovine blood with different concentrations of fibrinogen. Our findings indicate that spectroscopic techniques such as the Microplate Reader complement and expand the study of blood rheology, erythrocyte sedimentation and aggregation. |
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Microplate reader analysis of triatomine saliva effect on erythrocyte aggregationerythrocyte aggregationhemorheologyhematophagous salivaTriatoma infestansfibrinogenOur hypothesis is that the action of aggregating and disaggregating substances in the blood can be detected and quantified by the Microplate Reader. To ascertain the validity of this hypothesis, we selected two types of blood: one that naturally presents erythrocyte aggregation (pig blood) and the other that does not present aggregation (bovine blood). One important reason for the choice of pig blood is that its erythrocyte aggregation resembles that of human blood. T. infestans saliva was added to the pig blood as a disaggregating substance, while bovine fibrinogen was added to the bovine blood as a substance that promotes erythrocyte aggregation. We investigated the dynamic viscosity (h) of these mammals' blood, of T. infestans saliva and of the absorption (A) by Microplate Reader, carrying out UV-Vis spectrophotometric assays of pig plasma with different concentrations of triatominae saliva and of bovine blood with different concentrations of fibrinogen. Our findings indicate that spectroscopic techniques such as the Microplate Reader complement and expand the study of blood rheology, erythrocyte sedimentation and aggregation.ABM, ABC, ABPol2007-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392007000100008Materials Research v.10 n.1 2007reponame:Materials research (São Carlos. Online)instname:Universidade Federal de São Carlos (UFSCAR)instacron:ABM ABC ABPOL10.1590/S1516-14392007000100008info:eu-repo/semantics/openAccessCardoso,Antonio ValadãoPereira,Marcos HorácioMarcondes,Guilherme de AraújoFerreira,Adriana RosaAraújo,Patrícia Rosa deeng2007-05-03T00:00:00Zoai:scielo:S1516-14392007000100008Revistahttp://www.scielo.br/mrPUBhttps://old.scielo.br/oai/scielo-oai.phpdedz@power.ufscar.br1980-53731516-1439opendoar:2007-05-03T00:00Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.none.fl_str_mv |
Microplate reader analysis of triatomine saliva effect on erythrocyte aggregation |
title |
Microplate reader analysis of triatomine saliva effect on erythrocyte aggregation |
spellingShingle |
Microplate reader analysis of triatomine saliva effect on erythrocyte aggregation Cardoso,Antonio Valadão erythrocyte aggregation hemorheology hematophagous saliva Triatoma infestans fibrinogen |
title_short |
Microplate reader analysis of triatomine saliva effect on erythrocyte aggregation |
title_full |
Microplate reader analysis of triatomine saliva effect on erythrocyte aggregation |
title_fullStr |
Microplate reader analysis of triatomine saliva effect on erythrocyte aggregation |
title_full_unstemmed |
Microplate reader analysis of triatomine saliva effect on erythrocyte aggregation |
title_sort |
Microplate reader analysis of triatomine saliva effect on erythrocyte aggregation |
author |
Cardoso,Antonio Valadão |
author_facet |
Cardoso,Antonio Valadão Pereira,Marcos Horácio Marcondes,Guilherme de Araújo Ferreira,Adriana Rosa Araújo,Patrícia Rosa de |
author_role |
author |
author2 |
Pereira,Marcos Horácio Marcondes,Guilherme de Araújo Ferreira,Adriana Rosa Araújo,Patrícia Rosa de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Cardoso,Antonio Valadão Pereira,Marcos Horácio Marcondes,Guilherme de Araújo Ferreira,Adriana Rosa Araújo,Patrícia Rosa de |
dc.subject.por.fl_str_mv |
erythrocyte aggregation hemorheology hematophagous saliva Triatoma infestans fibrinogen |
topic |
erythrocyte aggregation hemorheology hematophagous saliva Triatoma infestans fibrinogen |
description |
Our hypothesis is that the action of aggregating and disaggregating substances in the blood can be detected and quantified by the Microplate Reader. To ascertain the validity of this hypothesis, we selected two types of blood: one that naturally presents erythrocyte aggregation (pig blood) and the other that does not present aggregation (bovine blood). One important reason for the choice of pig blood is that its erythrocyte aggregation resembles that of human blood. T. infestans saliva was added to the pig blood as a disaggregating substance, while bovine fibrinogen was added to the bovine blood as a substance that promotes erythrocyte aggregation. We investigated the dynamic viscosity (h) of these mammals' blood, of T. infestans saliva and of the absorption (A) by Microplate Reader, carrying out UV-Vis spectrophotometric assays of pig plasma with different concentrations of triatominae saliva and of bovine blood with different concentrations of fibrinogen. Our findings indicate that spectroscopic techniques such as the Microplate Reader complement and expand the study of blood rheology, erythrocyte sedimentation and aggregation. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392007000100008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392007000100008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-14392007000100008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
ABM, ABC, ABPol |
publisher.none.fl_str_mv |
ABM, ABC, ABPol |
dc.source.none.fl_str_mv |
Materials Research v.10 n.1 2007 reponame:Materials research (São Carlos. Online) instname:Universidade Federal de São Carlos (UFSCAR) instacron:ABM ABC ABPOL |
instname_str |
Universidade Federal de São Carlos (UFSCAR) |
instacron_str |
ABM ABC ABPOL |
institution |
ABM ABC ABPOL |
reponame_str |
Materials research (São Carlos. Online) |
collection |
Materials research (São Carlos. Online) |
repository.name.fl_str_mv |
Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR) |
repository.mail.fl_str_mv |
dedz@power.ufscar.br |
_version_ |
1754212658507153408 |