Astrocytes in paper chips and their interaction with hybrid vesicles

Detalhes bibliográficos
Autor(a) principal: Abild Meyer, Cathrine
Data de Publicação: 2022
Outros Autores: De Dios Andres, Paula, Brodszkij, Edit, Westensee, Isabella N., Lyons, Joseph, Vaz, Sandra H., Städler, Brigitte
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/56139
Resumo: © 2022 The Authors. Advanced Biology published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
id RCAP_51b90ceff4f44d2b8a8f313a8088f6e6
oai_identifier_str oai:repositorio.ul.pt:10451/56139
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Astrocytes in paper chips and their interaction with hybrid vesiclesAstrocytesCalcium signallingHybrid vesiclesPaper-based chips© 2022 The Authors. Advanced Biology published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.The role of astrocytes in brain function has received increased attention lately due to their critical role in brain development and function under physiological and pathophysiological conditions. However, the biological evaluation of soft material nanoparticles in astrocytes remains unexplored. Here, the interaction of crosslinked hybrid vesicles (HVs) and either C8-D1A astrocytes or primary astrocytes cultured in polystyrene tissue culture or floatable paper-based chips is investigated. The amphiphilic block copolymer poly(cholesteryl methacrylate)-block-poly(2-carboxyethyl acrylate) (P1) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine lipids are used for the assembly of HVs with crosslinked membranes. The assemblies show no short-term toxicity towards the C8-D1A astrocytes and the primary astrocytes, and both cell types internalize the HVs when cultured in 2D cell culture. Further, it is demonstrated that both the C8-D1A astrocytes and the primary astrocytes could mature in paper-based chips with preserved calcium signaling and glial fibrillary acidic protein expression. Last, it is confirmed that both types of astrocytes could internalize the HVs when cultured in paper-based chips. These findings lay out a fundamental understanding of the interaction between soft material nanoparticles and astrocytes, even when primary astrocytes are cultured in paper-based chips offering a 3D environment.This project was supported by the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement No. 818890), the Lundbeck Foundation (R346-2020-1617), and the Carlsberg Foundation (CF 20–0418). The HRM Queen Margrethe II's travel grant (C.A.M.) is acknowledged for support. TWileyRepositório da Universidade de LisboaAbild Meyer, CathrineDe Dios Andres, PaulaBrodszkij, EditWestensee, Isabella N.Lyons, JosephVaz, Sandra H.Städler, Brigitte2023-02-03T15:09:37Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/56139engAdv Biol (Weinh). 2023 Jan;7(1):e220020910.1002/adbi.2022002092701-0198info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:03:31Zoai:repositorio.ul.pt:10451/56139Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:06:40.628906Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Astrocytes in paper chips and their interaction with hybrid vesicles
title Astrocytes in paper chips and their interaction with hybrid vesicles
spellingShingle Astrocytes in paper chips and their interaction with hybrid vesicles
Abild Meyer, Cathrine
Astrocytes
Calcium signalling
Hybrid vesicles
Paper-based chips
title_short Astrocytes in paper chips and their interaction with hybrid vesicles
title_full Astrocytes in paper chips and their interaction with hybrid vesicles
title_fullStr Astrocytes in paper chips and their interaction with hybrid vesicles
title_full_unstemmed Astrocytes in paper chips and their interaction with hybrid vesicles
title_sort Astrocytes in paper chips and their interaction with hybrid vesicles
author Abild Meyer, Cathrine
author_facet Abild Meyer, Cathrine
De Dios Andres, Paula
Brodszkij, Edit
Westensee, Isabella N.
Lyons, Joseph
Vaz, Sandra H.
Städler, Brigitte
author_role author
author2 De Dios Andres, Paula
Brodszkij, Edit
Westensee, Isabella N.
Lyons, Joseph
Vaz, Sandra H.
Städler, Brigitte
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Abild Meyer, Cathrine
De Dios Andres, Paula
Brodszkij, Edit
Westensee, Isabella N.
Lyons, Joseph
Vaz, Sandra H.
Städler, Brigitte
dc.subject.por.fl_str_mv Astrocytes
Calcium signalling
Hybrid vesicles
Paper-based chips
topic Astrocytes
Calcium signalling
Hybrid vesicles
Paper-based chips
description © 2022 The Authors. Advanced Biology published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01T00:00:00Z
2023-02-03T15:09:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/56139
url http://hdl.handle.net/10451/56139
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Adv Biol (Weinh). 2023 Jan;7(1):e2200209
10.1002/adbi.202200209
2701-0198
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799134619990228992

Registros relacionados