Propolis efficacy: the quest for eco-friendly solvents

Detalhes bibliográficos
Autor(a) principal: Freitas, Ana Sofia
Data de Publicação: 2022
Outros Autores: Cunha, Ana, Parpot, Pier, Cardoso, Susana M., Oliveira, Rui Pedro Soares de, Almeida Aguiar, Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/80465
Resumo: Propolis, a natural product made by bees with resins and balsams, is known for its complex chemical composition and remarkable bioactivities. In this study, propolis extraction was studied seeking extracts with strong bioactivities using less orthodox solvents, with some derived from apiary products. For that, a propolis sample collected from Gerês apiary in 2018 (G18) was extracted by maceration with six different solvents: absolute ethanol, ethanol/water (7:3), honey brandy, mead, propylene glycol and water. The solvent influence on the chemical composition and antioxidant and antimicrobial activities of the extracts was investigated. Antioxidant potential was assessed by the DPPH free-radical-scavenging assay and the antimicrobial activity by the agar dilution method. Chemical composition of the extracts was determined in vitro by three colorimetric assays: total ortho-diphenols, phenolics and flavonoids contents and the LC-MS technique. To our knowledge, this is the first time that solvents such as honey brandy and mead have been studied. Honey brandy showed considerable potential to extract propolis active compounds able to inhibit the growth of bacteria such as the methicillin-sensitive Staphylococcus aureus and Propionibacterium acnes (MIC values of 100 and 200 µg/mL, respectively) and the fungi Candida albicans and Saccharomyces cerevisiae (MIC=500 µg/mL, for both). Mead extracts displayed high antioxidant capacity (EC50=1.63 ± 0.27 µg/mL) and great activity against resistant bacteria such as the methicillin-resistant Staphylococcus aureus and Escherichia coli (MIC=750 µg/mL, for both). The production of such solvents made from beehive products further promotes a diversification of apiary products and the exploration of new applications using eco-friendly solutions.
id RCAP_52a8c235ab78483b58b7273ce6e0a067
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/80465
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Propolis efficacy: the quest for eco-friendly solventspropolis extractionpropolis extraction solventsphenolic compoundsantibacterial activityantioxidant potentialpropolisScience & TechnologyPropolis, a natural product made by bees with resins and balsams, is known for its complex chemical composition and remarkable bioactivities. In this study, propolis extraction was studied seeking extracts with strong bioactivities using less orthodox solvents, with some derived from apiary products. For that, a propolis sample collected from Gerês apiary in 2018 (G18) was extracted by maceration with six different solvents: absolute ethanol, ethanol/water (7:3), honey brandy, mead, propylene glycol and water. The solvent influence on the chemical composition and antioxidant and antimicrobial activities of the extracts was investigated. Antioxidant potential was assessed by the DPPH free-radical-scavenging assay and the antimicrobial activity by the agar dilution method. Chemical composition of the extracts was determined in vitro by three colorimetric assays: total ortho-diphenols, phenolics and flavonoids contents and the LC-MS technique. To our knowledge, this is the first time that solvents such as honey brandy and mead have been studied. Honey brandy showed considerable potential to extract propolis active compounds able to inhibit the growth of bacteria such as the methicillin-sensitive Staphylococcus aureus and Propionibacterium acnes (MIC values of 100 and 200 µg/mL, respectively) and the fungi Candida albicans and Saccharomyces cerevisiae (MIC=500 µg/mL, for both). Mead extracts displayed high antioxidant capacity (EC50=1.63 ± 0.27 µg/mL) and great activity against resistant bacteria such as the methicillin-resistant Staphylococcus aureus and Escherichia coli (MIC=750 µg/mL, for both). The production of such solvents made from beehive products further promotes a diversification of apiary products and the exploration of new applications using eco-friendly solutions.Ana Freitas acknowledges the financial support provided by national funds through FCT—Portuguese Foundation for Science and Technology (PD/BD/128276/2017), under the Doctoral Programme “Agricultural Production Chains—from fork to farm” (PD/00122/2012), and from the European Social Funds and the Regional Operational Programme Norte 2020. This study was also supported by CITAB research unit (UIDB/04033/2020), CBMA research unit (Contrato-Programa UIDB/04050/2020), Centre of Chemistry (UID/QUI/0686/2016), BioTecNorte (operation NORTE-01-0145-FEDER-000004), supported by the Northern Portugal Regional Operational Programme (NORTE 2020), LAQV (projects UIDB/50006/2020, UIDP/50006/2020, and UID/BIA/04004/2020) funded by national funds through the FCT I.P.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoFreitas, Ana SofiaCunha, AnaParpot, PierCardoso, Susana M.Oliveira, Rui Pedro Soares deAlmeida Aguiar, Cristina2022-11-032022-11-03T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/80465engFreitas, A.S.; Cunha, A.; Parpot, P.; Cardoso, S.M.; Oliveira, R.; Almeida-Aguiar, C. Propolis Efficacy: The Quest for Eco-Friendly Solvents. Molecules 2022, 27, 7531. https://doi.org/10.3390/molecules272175311420-304910.3390/molecules2721753136364353https://www.mdpi.com/1420-3049/27/21/7531info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T05:07:19Zoai:repositorium.sdum.uminho.pt:1822/80465Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T05:07:19Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Propolis efficacy: the quest for eco-friendly solvents
title Propolis efficacy: the quest for eco-friendly solvents
spellingShingle Propolis efficacy: the quest for eco-friendly solvents
Freitas, Ana Sofia
propolis extraction
propolis extraction solvents
phenolic compounds
antibacterial activity
antioxidant potential
propolis
Science & Technology
title_short Propolis efficacy: the quest for eco-friendly solvents
title_full Propolis efficacy: the quest for eco-friendly solvents
title_fullStr Propolis efficacy: the quest for eco-friendly solvents
title_full_unstemmed Propolis efficacy: the quest for eco-friendly solvents
title_sort Propolis efficacy: the quest for eco-friendly solvents
author Freitas, Ana Sofia
author_facet Freitas, Ana Sofia
Cunha, Ana
Parpot, Pier
Cardoso, Susana M.
Oliveira, Rui Pedro Soares de
Almeida Aguiar, Cristina
author_role author
author2 Cunha, Ana
Parpot, Pier
Cardoso, Susana M.
Oliveira, Rui Pedro Soares de
Almeida Aguiar, Cristina
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Freitas, Ana Sofia
Cunha, Ana
Parpot, Pier
Cardoso, Susana M.
Oliveira, Rui Pedro Soares de
Almeida Aguiar, Cristina
dc.subject.por.fl_str_mv propolis extraction
propolis extraction solvents
phenolic compounds
antibacterial activity
antioxidant potential
propolis
Science & Technology
topic propolis extraction
propolis extraction solvents
phenolic compounds
antibacterial activity
antioxidant potential
propolis
Science & Technology
description Propolis, a natural product made by bees with resins and balsams, is known for its complex chemical composition and remarkable bioactivities. In this study, propolis extraction was studied seeking extracts with strong bioactivities using less orthodox solvents, with some derived from apiary products. For that, a propolis sample collected from Gerês apiary in 2018 (G18) was extracted by maceration with six different solvents: absolute ethanol, ethanol/water (7:3), honey brandy, mead, propylene glycol and water. The solvent influence on the chemical composition and antioxidant and antimicrobial activities of the extracts was investigated. Antioxidant potential was assessed by the DPPH free-radical-scavenging assay and the antimicrobial activity by the agar dilution method. Chemical composition of the extracts was determined in vitro by three colorimetric assays: total ortho-diphenols, phenolics and flavonoids contents and the LC-MS technique. To our knowledge, this is the first time that solvents such as honey brandy and mead have been studied. Honey brandy showed considerable potential to extract propolis active compounds able to inhibit the growth of bacteria such as the methicillin-sensitive Staphylococcus aureus and Propionibacterium acnes (MIC values of 100 and 200 µg/mL, respectively) and the fungi Candida albicans and Saccharomyces cerevisiae (MIC=500 µg/mL, for both). Mead extracts displayed high antioxidant capacity (EC50=1.63 ± 0.27 µg/mL) and great activity against resistant bacteria such as the methicillin-resistant Staphylococcus aureus and Escherichia coli (MIC=750 µg/mL, for both). The production of such solvents made from beehive products further promotes a diversification of apiary products and the exploration of new applications using eco-friendly solutions.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-03
2022-11-03T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/80465
url https://hdl.handle.net/1822/80465
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Freitas, A.S.; Cunha, A.; Parpot, P.; Cardoso, S.M.; Oliveira, R.; Almeida-Aguiar, C. Propolis Efficacy: The Quest for Eco-Friendly Solvents. Molecules 2022, 27, 7531. https://doi.org/10.3390/molecules27217531
1420-3049
10.3390/molecules27217531
36364353
https://www.mdpi.com/1420-3049/27/21/7531
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817544521789997056