Propolis efficacy: the quest for eco-friendly solvents
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/80465 |
Resumo: | Propolis, a natural product made by bees with resins and balsams, is known for its complex chemical composition and remarkable bioactivities. In this study, propolis extraction was studied seeking extracts with strong bioactivities using less orthodox solvents, with some derived from apiary products. For that, a propolis sample collected from Gerês apiary in 2018 (G18) was extracted by maceration with six different solvents: absolute ethanol, ethanol/water (7:3), honey brandy, mead, propylene glycol and water. The solvent influence on the chemical composition and antioxidant and antimicrobial activities of the extracts was investigated. Antioxidant potential was assessed by the DPPH free-radical-scavenging assay and the antimicrobial activity by the agar dilution method. Chemical composition of the extracts was determined in vitro by three colorimetric assays: total ortho-diphenols, phenolics and flavonoids contents and the LC-MS technique. To our knowledge, this is the first time that solvents such as honey brandy and mead have been studied. Honey brandy showed considerable potential to extract propolis active compounds able to inhibit the growth of bacteria such as the methicillin-sensitive Staphylococcus aureus and Propionibacterium acnes (MIC values of 100 and 200 µg/mL, respectively) and the fungi Candida albicans and Saccharomyces cerevisiae (MIC=500 µg/mL, for both). Mead extracts displayed high antioxidant capacity (EC50=1.63 ± 0.27 µg/mL) and great activity against resistant bacteria such as the methicillin-resistant Staphylococcus aureus and Escherichia coli (MIC=750 µg/mL, for both). The production of such solvents made from beehive products further promotes a diversification of apiary products and the exploration of new applications using eco-friendly solutions. |
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Propolis efficacy: the quest for eco-friendly solventspropolis extractionpropolis extraction solventsphenolic compoundsantibacterial activityantioxidant potentialpropolisScience & TechnologyPropolis, a natural product made by bees with resins and balsams, is known for its complex chemical composition and remarkable bioactivities. In this study, propolis extraction was studied seeking extracts with strong bioactivities using less orthodox solvents, with some derived from apiary products. For that, a propolis sample collected from Gerês apiary in 2018 (G18) was extracted by maceration with six different solvents: absolute ethanol, ethanol/water (7:3), honey brandy, mead, propylene glycol and water. The solvent influence on the chemical composition and antioxidant and antimicrobial activities of the extracts was investigated. Antioxidant potential was assessed by the DPPH free-radical-scavenging assay and the antimicrobial activity by the agar dilution method. Chemical composition of the extracts was determined in vitro by three colorimetric assays: total ortho-diphenols, phenolics and flavonoids contents and the LC-MS technique. To our knowledge, this is the first time that solvents such as honey brandy and mead have been studied. Honey brandy showed considerable potential to extract propolis active compounds able to inhibit the growth of bacteria such as the methicillin-sensitive Staphylococcus aureus and Propionibacterium acnes (MIC values of 100 and 200 µg/mL, respectively) and the fungi Candida albicans and Saccharomyces cerevisiae (MIC=500 µg/mL, for both). Mead extracts displayed high antioxidant capacity (EC50=1.63 ± 0.27 µg/mL) and great activity against resistant bacteria such as the methicillin-resistant Staphylococcus aureus and Escherichia coli (MIC=750 µg/mL, for both). The production of such solvents made from beehive products further promotes a diversification of apiary products and the exploration of new applications using eco-friendly solutions.Ana Freitas acknowledges the financial support provided by national funds through FCT—Portuguese Foundation for Science and Technology (PD/BD/128276/2017), under the Doctoral Programme “Agricultural Production Chains—from fork to farm” (PD/00122/2012), and from the European Social Funds and the Regional Operational Programme Norte 2020. This study was also supported by CITAB research unit (UIDB/04033/2020), CBMA research unit (Contrato-Programa UIDB/04050/2020), Centre of Chemistry (UID/QUI/0686/2016), BioTecNorte (operation NORTE-01-0145-FEDER-000004), supported by the Northern Portugal Regional Operational Programme (NORTE 2020), LAQV (projects UIDB/50006/2020, UIDP/50006/2020, and UID/BIA/04004/2020) funded by national funds through the FCT I.P.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoFreitas, Ana SofiaCunha, AnaParpot, PierCardoso, Susana M.Oliveira, Rui Pedro Soares deAlmeida Aguiar, Cristina2022-11-032022-11-03T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/80465engFreitas, A.S.; Cunha, A.; Parpot, P.; Cardoso, S.M.; Oliveira, R.; Almeida-Aguiar, C. Propolis Efficacy: The Quest for Eco-Friendly Solvents. Molecules 2022, 27, 7531. https://doi.org/10.3390/molecules272175311420-304910.3390/molecules2721753136364353https://www.mdpi.com/1420-3049/27/21/7531info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T05:07:19Zoai:repositorium.sdum.uminho.pt:1822/80465Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T05:07:19Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Propolis efficacy: the quest for eco-friendly solvents |
title |
Propolis efficacy: the quest for eco-friendly solvents |
spellingShingle |
Propolis efficacy: the quest for eco-friendly solvents Freitas, Ana Sofia propolis extraction propolis extraction solvents phenolic compounds antibacterial activity antioxidant potential propolis Science & Technology |
title_short |
Propolis efficacy: the quest for eco-friendly solvents |
title_full |
Propolis efficacy: the quest for eco-friendly solvents |
title_fullStr |
Propolis efficacy: the quest for eco-friendly solvents |
title_full_unstemmed |
Propolis efficacy: the quest for eco-friendly solvents |
title_sort |
Propolis efficacy: the quest for eco-friendly solvents |
author |
Freitas, Ana Sofia |
author_facet |
Freitas, Ana Sofia Cunha, Ana Parpot, Pier Cardoso, Susana M. Oliveira, Rui Pedro Soares de Almeida Aguiar, Cristina |
author_role |
author |
author2 |
Cunha, Ana Parpot, Pier Cardoso, Susana M. Oliveira, Rui Pedro Soares de Almeida Aguiar, Cristina |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Freitas, Ana Sofia Cunha, Ana Parpot, Pier Cardoso, Susana M. Oliveira, Rui Pedro Soares de Almeida Aguiar, Cristina |
dc.subject.por.fl_str_mv |
propolis extraction propolis extraction solvents phenolic compounds antibacterial activity antioxidant potential propolis Science & Technology |
topic |
propolis extraction propolis extraction solvents phenolic compounds antibacterial activity antioxidant potential propolis Science & Technology |
description |
Propolis, a natural product made by bees with resins and balsams, is known for its complex chemical composition and remarkable bioactivities. In this study, propolis extraction was studied seeking extracts with strong bioactivities using less orthodox solvents, with some derived from apiary products. For that, a propolis sample collected from Gerês apiary in 2018 (G18) was extracted by maceration with six different solvents: absolute ethanol, ethanol/water (7:3), honey brandy, mead, propylene glycol and water. The solvent influence on the chemical composition and antioxidant and antimicrobial activities of the extracts was investigated. Antioxidant potential was assessed by the DPPH free-radical-scavenging assay and the antimicrobial activity by the agar dilution method. Chemical composition of the extracts was determined in vitro by three colorimetric assays: total ortho-diphenols, phenolics and flavonoids contents and the LC-MS technique. To our knowledge, this is the first time that solvents such as honey brandy and mead have been studied. Honey brandy showed considerable potential to extract propolis active compounds able to inhibit the growth of bacteria such as the methicillin-sensitive Staphylococcus aureus and Propionibacterium acnes (MIC values of 100 and 200 µg/mL, respectively) and the fungi Candida albicans and Saccharomyces cerevisiae (MIC=500 µg/mL, for both). Mead extracts displayed high antioxidant capacity (EC50=1.63 ± 0.27 µg/mL) and great activity against resistant bacteria such as the methicillin-resistant Staphylococcus aureus and Escherichia coli (MIC=750 µg/mL, for both). The production of such solvents made from beehive products further promotes a diversification of apiary products and the exploration of new applications using eco-friendly solutions. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-03 2022-11-03T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/80465 |
url |
https://hdl.handle.net/1822/80465 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Freitas, A.S.; Cunha, A.; Parpot, P.; Cardoso, S.M.; Oliveira, R.; Almeida-Aguiar, C. Propolis Efficacy: The Quest for Eco-Friendly Solvents. Molecules 2022, 27, 7531. https://doi.org/10.3390/molecules27217531 1420-3049 10.3390/molecules27217531 36364353 https://www.mdpi.com/1420-3049/27/21/7531 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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mluisa.alvim@gmail.com |
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