Cellulose morphology and enzymatic reactivity: a modified solute exclusion technique

Detalhes bibliográficos
Autor(a) principal: Gama, F. M.
Data de Publicação: 1994
Outros Autores: Teixeira, J. A., Mota, M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/41782
Resumo: An expeditious and accurate simplification of Stone and Scallan's solute exclusion technique was developed, thereby avoiding several sources of experimental error coupled with the determination of cellulose pore volume. Using this method, it is shown that cellulolytic enzymes do not enter into the micropores of five studied celluloses. These results suggestes that hydrolysis occurs initially at the external surface of the fibers. This surface area was calculated with the help of adsorption isotherms of bovine serum albumin. The obtained values for the different samples agree with the microscopically observed cellulose morphology. The correlation obtained by several authors relating cellulose porosity and its digestibility is explained as a consequence of the lower crystallinity and easier fragmentation of the more porous celluloses during hydrolysis.
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spelling Cellulose morphology and enzymatic reactivity: a modified solute exclusion techniqueCellulose morphologyCellulasePoresSurface areaScience & TechnologyAn expeditious and accurate simplification of Stone and Scallan's solute exclusion technique was developed, thereby avoiding several sources of experimental error coupled with the determination of cellulose pore volume. Using this method, it is shown that cellulolytic enzymes do not enter into the micropores of five studied celluloses. These results suggestes that hydrolysis occurs initially at the external surface of the fibers. This surface area was calculated with the help of adsorption isotherms of bovine serum albumin. The obtained values for the different samples agree with the microscopically observed cellulose morphology. The correlation obtained by several authors relating cellulose porosity and its digestibility is explained as a consequence of the lower crystallinity and easier fragmentation of the more porous celluloses during hydrolysis.John Wiley and SonsUniversidade do MinhoGama, F. M.Teixeira, J. A.Mota, M.19941994-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/41782engGama, F. M.; Teixeira, J. A.; Mota, M., Cellulose morphology and enzymatic reactivity: A modified solute exclusion technique. Biotechnology and Bioengineering, 43(5), 381-387, 19940006-35921097-029010.1002/bit.260430506http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0290info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T06:38:59Zoai:repositorium.sdum.uminho.pt:1822/41782Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T06:38:59Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cellulose morphology and enzymatic reactivity: a modified solute exclusion technique
title Cellulose morphology and enzymatic reactivity: a modified solute exclusion technique
spellingShingle Cellulose morphology and enzymatic reactivity: a modified solute exclusion technique
Gama, F. M.
Cellulose morphology
Cellulase
Pores
Surface area
Science & Technology
title_short Cellulose morphology and enzymatic reactivity: a modified solute exclusion technique
title_full Cellulose morphology and enzymatic reactivity: a modified solute exclusion technique
title_fullStr Cellulose morphology and enzymatic reactivity: a modified solute exclusion technique
title_full_unstemmed Cellulose morphology and enzymatic reactivity: a modified solute exclusion technique
title_sort Cellulose morphology and enzymatic reactivity: a modified solute exclusion technique
author Gama, F. M.
author_facet Gama, F. M.
Teixeira, J. A.
Mota, M.
author_role author
author2 Teixeira, J. A.
Mota, M.
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gama, F. M.
Teixeira, J. A.
Mota, M.
dc.subject.por.fl_str_mv Cellulose morphology
Cellulase
Pores
Surface area
Science & Technology
topic Cellulose morphology
Cellulase
Pores
Surface area
Science & Technology
description An expeditious and accurate simplification of Stone and Scallan's solute exclusion technique was developed, thereby avoiding several sources of experimental error coupled with the determination of cellulose pore volume. Using this method, it is shown that cellulolytic enzymes do not enter into the micropores of five studied celluloses. These results suggestes that hydrolysis occurs initially at the external surface of the fibers. This surface area was calculated with the help of adsorption isotherms of bovine serum albumin. The obtained values for the different samples agree with the microscopically observed cellulose morphology. The correlation obtained by several authors relating cellulose porosity and its digestibility is explained as a consequence of the lower crystallinity and easier fragmentation of the more porous celluloses during hydrolysis.
publishDate 1994
dc.date.none.fl_str_mv 1994
1994-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/41782
url http://hdl.handle.net/1822/41782
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gama, F. M.; Teixeira, J. A.; Mota, M., Cellulose morphology and enzymatic reactivity: A modified solute exclusion technique. Biotechnology and Bioengineering, 43(5), 381-387, 1994
0006-3592
1097-0290
10.1002/bit.260430506
http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0290
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley and Sons
publisher.none.fl_str_mv John Wiley and Sons
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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