Extracellular vesicles derived from osteogenically induced human bone marrow mesenchymal stem cells can modulate lineage commitment
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/43825 |
Resumo: | The effective osteogenic commitment of human bone marrow mesenchymal stem cells (hBMSCs) is critical for bone regenerative therapies. Extracellular vesicles (EVs) derived from hBMSCs have a regenerative potential that has been increasingly recognized. Herein, the osteoinductive potential of osteogenically induced hBMSC-EVs was examined. hBMSCs secreted negatively charged nanosized vesicles (⠼35 nm) with EV-related surface markers. The yield of EVs over 7 days was dependent on an osteogenic stimulus (standard chemical cocktail or RUNX2 cationic-lipid transfection). These EVs were used to sequentially stimulate homotypic uncommitted cells during 7 days, matching the seeding density of EV parent cells, culture time, and stimuli. Osteogenically committed hBMSC-EVs induced an osteogenic phenotype characterized by marked early induction of BMP2, SP7, SPP1, BGLAP/IBSP, and alkaline phosphatase. Both EV groups outperformed the currently used osteoinductive strategies. These data show that naturally secreted EVs can guide the osteogenic commitment of hBMSCs in the absence of other chemical or genetic osteoinductors. |
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Extracellular vesicles derived from osteogenically induced human bone marrow mesenchymal stem cells can modulate lineage commitmentAdvanced therapies/ bone regenerationDifferentiationExosomeOsteogenic commitment strategiesSecretomeStem cellsScience & TechnologyThe effective osteogenic commitment of human bone marrow mesenchymal stem cells (hBMSCs) is critical for bone regenerative therapies. Extracellular vesicles (EVs) derived from hBMSCs have a regenerative potential that has been increasingly recognized. Herein, the osteoinductive potential of osteogenically induced hBMSC-EVs was examined. hBMSCs secreted negatively charged nanosized vesicles (â ¼35 nm) with EV-related surface markers. The yield of EVs over 7 days was dependent on an osteogenic stimulus (standard chemical cocktail or RUNX2 cationic-lipid transfection). These EVs were used to sequentially stimulate homotypic uncommitted cells during 7 days, matching the seeding density of EV parent cells, culture time, and stimuli. Osteogenically committed hBMSC-EVs induced an osteogenic phenotype characterized by marked early induction of BMP2, SP7, SPP1, BGLAP/IBSP, and alkaline phosphatase. Both EV groups outperformed the currently used osteoinductive strategies. These data show that naturally secreted EVs can guide the osteogenic commitment of hBMSCs in the absence of other chemical or genetic osteoinductors.The authors thank the financial support of QREN (RL1-ABMRNORTE-01-0124-FEDER-000016 and RL3–TECT–NORTE-01-0124- FEDER-000020) co-financed by North Portugal Regional Operational Program (ON.2–O Novo Norte), under the NSRF, through the ERDF; the European Union’s Seventh Framework Program (FP7/2007–2013), Grant No. REGPOT-CT2012-316331- POLARIS; and the laboratory assistance of Elsa Ribeiro, Magda Graça, and Sónia Zacarias.Cell PressUniversidade do MinhoMartins, Margarida Isabel Barros CoelhoRibeiro, Diana Margarida da CostaMartins, AlbinoReis, R. L.Neves, N. M.2016-032016-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/43825engMartins M., Ribeiro D., Martins A., Reis R. L., Neves N. M. Extracellular Vesicles Derived from Osteogenically Induced Human Bone Marrow Mesenchymal Stem Cells Can Modulate Lineage Commitment, Stem Cell Reports , Vol. 6, Issue 3, pp. 284-291, doi:10.1016/j.stemcr.2016.01.001, 20162213-671110.1016/j.stemcr.2016.01.00126923821http://www.cell.com/stem-cell-reports/pdf/S2213-6711(16)00002-3.pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:14:48Zoai:repositorium.sdum.uminho.pt:1822/43825Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:07:09.579026Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Extracellular vesicles derived from osteogenically induced human bone marrow mesenchymal stem cells can modulate lineage commitment |
title |
Extracellular vesicles derived from osteogenically induced human bone marrow mesenchymal stem cells can modulate lineage commitment |
spellingShingle |
Extracellular vesicles derived from osteogenically induced human bone marrow mesenchymal stem cells can modulate lineage commitment Martins, Margarida Isabel Barros Coelho Advanced therapies/ bone regeneration Differentiation Exosome Osteogenic commitment strategies Secretome Stem cells Science & Technology |
title_short |
Extracellular vesicles derived from osteogenically induced human bone marrow mesenchymal stem cells can modulate lineage commitment |
title_full |
Extracellular vesicles derived from osteogenically induced human bone marrow mesenchymal stem cells can modulate lineage commitment |
title_fullStr |
Extracellular vesicles derived from osteogenically induced human bone marrow mesenchymal stem cells can modulate lineage commitment |
title_full_unstemmed |
Extracellular vesicles derived from osteogenically induced human bone marrow mesenchymal stem cells can modulate lineage commitment |
title_sort |
Extracellular vesicles derived from osteogenically induced human bone marrow mesenchymal stem cells can modulate lineage commitment |
author |
Martins, Margarida Isabel Barros Coelho |
author_facet |
Martins, Margarida Isabel Barros Coelho Ribeiro, Diana Margarida da Costa Martins, Albino Reis, R. L. Neves, N. M. |
author_role |
author |
author2 |
Ribeiro, Diana Margarida da Costa Martins, Albino Reis, R. L. Neves, N. M. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Martins, Margarida Isabel Barros Coelho Ribeiro, Diana Margarida da Costa Martins, Albino Reis, R. L. Neves, N. M. |
dc.subject.por.fl_str_mv |
Advanced therapies/ bone regeneration Differentiation Exosome Osteogenic commitment strategies Secretome Stem cells Science & Technology |
topic |
Advanced therapies/ bone regeneration Differentiation Exosome Osteogenic commitment strategies Secretome Stem cells Science & Technology |
description |
The effective osteogenic commitment of human bone marrow mesenchymal stem cells (hBMSCs) is critical for bone regenerative therapies. Extracellular vesicles (EVs) derived from hBMSCs have a regenerative potential that has been increasingly recognized. Herein, the osteoinductive potential of osteogenically induced hBMSC-EVs was examined. hBMSCs secreted negatively charged nanosized vesicles (⠼35 nm) with EV-related surface markers. The yield of EVs over 7 days was dependent on an osteogenic stimulus (standard chemical cocktail or RUNX2 cationic-lipid transfection). These EVs were used to sequentially stimulate homotypic uncommitted cells during 7 days, matching the seeding density of EV parent cells, culture time, and stimuli. Osteogenically committed hBMSC-EVs induced an osteogenic phenotype characterized by marked early induction of BMP2, SP7, SPP1, BGLAP/IBSP, and alkaline phosphatase. Both EV groups outperformed the currently used osteoinductive strategies. These data show that naturally secreted EVs can guide the osteogenic commitment of hBMSCs in the absence of other chemical or genetic osteoinductors. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03 2016-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/43825 |
url |
http://hdl.handle.net/1822/43825 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Martins M., Ribeiro D., Martins A., Reis R. L., Neves N. M. Extracellular Vesicles Derived from Osteogenically Induced Human Bone Marrow Mesenchymal Stem Cells Can Modulate Lineage Commitment, Stem Cell Reports , Vol. 6, Issue 3, pp. 284-291, doi:10.1016/j.stemcr.2016.01.001, 2016 2213-6711 10.1016/j.stemcr.2016.01.001 26923821 http://www.cell.com/stem-cell-reports/pdf/S2213-6711(16)00002-3.pdf |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Cell Press |
publisher.none.fl_str_mv |
Cell Press |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132488782577664 |