Sulfate-based lipids: analysis of healthy human fluids and cell extracts
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/37516 |
Resumo: | Sulfate-based lipids (SL) have been proposed as players in inflammation, immunity and infection. In spite of the many biochemical processes linked to SL, analysis on this class of lipids has only focused on specific SL sub-classes in individual fluids or cells leaving a range of additional SL in other biological samples unaccounted for. This study describes the mass spectrometry screening of SL in lipid extracts of human fluids (saliva, plasma, urine, seminal fluid) and primary human cells (RBC, neutrophils, fibroblasts and skin epidermal) using targeted precursor ion scanning (PIS) approach. The PIS 97 mass spectra reveal a wide diversity of SL including steroid sulfates, sulfoglycolipids and other unidentified SL, as well as metabolites such as taurines, sulfated polyphenols and hypurate conjugates. Semi-quantification of SL revealed that plasma exhibited the highest content of SL whereas seminal fluid and epithelial cells contained the highest sulphur to phosphorous (S/P) ratio. The complexity of biofluids and cells sulfateome presented in this study highlight the importance of expanding the panel of synthetic sulfate-based lipid standards. Also, the heterogenous distribution of SL provides evidence for the interplay of sulfotransferases/sulfatases, opening new avenues for biomarker discovery in oral health, cardiovascular, fertility and dermatology research areas. |
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Sulfate-based lipids: analysis of healthy human fluids and cell extractsCholesterol sulfateSulfoglycolipidsTaurine conjugatesSeminal fluidElectronegative LDLPolyphenol metabolitesHypurate conjugatesSulfate-based lipids (SL) have been proposed as players in inflammation, immunity and infection. In spite of the many biochemical processes linked to SL, analysis on this class of lipids has only focused on specific SL sub-classes in individual fluids or cells leaving a range of additional SL in other biological samples unaccounted for. This study describes the mass spectrometry screening of SL in lipid extracts of human fluids (saliva, plasma, urine, seminal fluid) and primary human cells (RBC, neutrophils, fibroblasts and skin epidermal) using targeted precursor ion scanning (PIS) approach. The PIS 97 mass spectra reveal a wide diversity of SL including steroid sulfates, sulfoglycolipids and other unidentified SL, as well as metabolites such as taurines, sulfated polyphenols and hypurate conjugates. Semi-quantification of SL revealed that plasma exhibited the highest content of SL whereas seminal fluid and epithelial cells contained the highest sulphur to phosphorous (S/P) ratio. The complexity of biofluids and cells sulfateome presented in this study highlight the importance of expanding the panel of synthetic sulfate-based lipid standards. Also, the heterogenous distribution of SL provides evidence for the interplay of sulfotransferases/sulfatases, opening new avenues for biomarker discovery in oral health, cardiovascular, fertility and dermatology research areas.Elsevier2023-05-05T08:20:45Z2019-07-01T00:00:00Z2019-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/37516eng0009-308410.1016/j.chemphyslip.2019.03.009Dias, Irundika H. K.Ferreira, RitaGruber, FlorianVitorino, RuiRivas-Urbina, AndreaSanchez-Quesada, José LuisVieira Silva, JoanaFardilha, Margaridade Freitas, VictorReis, Anainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:12:31Zoai:ria.ua.pt:10773/37516Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:08:08.508207Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Sulfate-based lipids: analysis of healthy human fluids and cell extracts |
title |
Sulfate-based lipids: analysis of healthy human fluids and cell extracts |
spellingShingle |
Sulfate-based lipids: analysis of healthy human fluids and cell extracts Dias, Irundika H. K. Cholesterol sulfate Sulfoglycolipids Taurine conjugates Seminal fluid Electronegative LDL Polyphenol metabolites Hypurate conjugates |
title_short |
Sulfate-based lipids: analysis of healthy human fluids and cell extracts |
title_full |
Sulfate-based lipids: analysis of healthy human fluids and cell extracts |
title_fullStr |
Sulfate-based lipids: analysis of healthy human fluids and cell extracts |
title_full_unstemmed |
Sulfate-based lipids: analysis of healthy human fluids and cell extracts |
title_sort |
Sulfate-based lipids: analysis of healthy human fluids and cell extracts |
author |
Dias, Irundika H. K. |
author_facet |
Dias, Irundika H. K. Ferreira, Rita Gruber, Florian Vitorino, Rui Rivas-Urbina, Andrea Sanchez-Quesada, José Luis Vieira Silva, Joana Fardilha, Margarida de Freitas, Victor Reis, Ana |
author_role |
author |
author2 |
Ferreira, Rita Gruber, Florian Vitorino, Rui Rivas-Urbina, Andrea Sanchez-Quesada, José Luis Vieira Silva, Joana Fardilha, Margarida de Freitas, Victor Reis, Ana |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Dias, Irundika H. K. Ferreira, Rita Gruber, Florian Vitorino, Rui Rivas-Urbina, Andrea Sanchez-Quesada, José Luis Vieira Silva, Joana Fardilha, Margarida de Freitas, Victor Reis, Ana |
dc.subject.por.fl_str_mv |
Cholesterol sulfate Sulfoglycolipids Taurine conjugates Seminal fluid Electronegative LDL Polyphenol metabolites Hypurate conjugates |
topic |
Cholesterol sulfate Sulfoglycolipids Taurine conjugates Seminal fluid Electronegative LDL Polyphenol metabolites Hypurate conjugates |
description |
Sulfate-based lipids (SL) have been proposed as players in inflammation, immunity and infection. In spite of the many biochemical processes linked to SL, analysis on this class of lipids has only focused on specific SL sub-classes in individual fluids or cells leaving a range of additional SL in other biological samples unaccounted for. This study describes the mass spectrometry screening of SL in lipid extracts of human fluids (saliva, plasma, urine, seminal fluid) and primary human cells (RBC, neutrophils, fibroblasts and skin epidermal) using targeted precursor ion scanning (PIS) approach. The PIS 97 mass spectra reveal a wide diversity of SL including steroid sulfates, sulfoglycolipids and other unidentified SL, as well as metabolites such as taurines, sulfated polyphenols and hypurate conjugates. Semi-quantification of SL revealed that plasma exhibited the highest content of SL whereas seminal fluid and epithelial cells contained the highest sulphur to phosphorous (S/P) ratio. The complexity of biofluids and cells sulfateome presented in this study highlight the importance of expanding the panel of synthetic sulfate-based lipid standards. Also, the heterogenous distribution of SL provides evidence for the interplay of sulfotransferases/sulfatases, opening new avenues for biomarker discovery in oral health, cardiovascular, fertility and dermatology research areas. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-07-01T00:00:00Z 2019-07 2023-05-05T08:20:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/37516 |
url |
http://hdl.handle.net/10773/37516 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0009-3084 10.1016/j.chemphyslip.2019.03.009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137735081984000 |