Isolation and purification of an enzyme hydrolyzing ochratoxin A from aspergillus niger

Detalhes bibliográficos
Autor(a) principal: Abrunhosa, Luís
Data de Publicação: 2007
Outros Autores: Venâncio, Armando
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/7472
Resumo: Ochratoxin A is a mycotoxin produced by several Aspergillus and some Penicillium species which may be present in food and feed products. It can be enzymatically hydrolyzed into ochratoxin α and l-β-phenylalanine, thereby decreasing its toxicity. The ochratoxin A degradation capacity of Aspergillus niger is well known and here we report the isolation and purification of a novel enzyme from A. niger that hydrolyzes this mycotoxin. A wheat germ medium supplemented with ochratoxin A was used to produce the enzyme, which was purified from culture filtrate by acetone precipitation and anion exchange chromatography. An overall purification of 2.5-fold with a recovery of 68% and a final specific activity of 36 U/mg was obtained. The enzyme is a metalloenzyme as it was inhibited at 10 mM EDTA, whereas PMSF had no effect. The ochratoxin A hydrolytic enzyme presented a V max of 0.44 μM/min and a K m of 0.5 mM when the reaction was carried out at pH 7.5 and 37°C.
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spelling Isolation and purification of an enzyme hydrolyzing ochratoxin A from aspergillus nigerAspergillus nigerCarboxypeptidaseDegradationOchratoxin AOchratoxin αScience & TechnologyOchratoxin A is a mycotoxin produced by several Aspergillus and some Penicillium species which may be present in food and feed products. It can be enzymatically hydrolyzed into ochratoxin α and l-β-phenylalanine, thereby decreasing its toxicity. The ochratoxin A degradation capacity of Aspergillus niger is well known and here we report the isolation and purification of a novel enzyme from A. niger that hydrolyzes this mycotoxin. A wheat germ medium supplemented with ochratoxin A was used to produce the enzyme, which was purified from culture filtrate by acetone precipitation and anion exchange chromatography. An overall purification of 2.5-fold with a recovery of 68% and a final specific activity of 36 U/mg was obtained. The enzyme is a metalloenzyme as it was inhibited at 10 mM EDTA, whereas PMSF had no effect. The ochratoxin A hydrolytic enzyme presented a V max of 0.44 μM/min and a K m of 0.5 mM when the reaction was carried out at pH 7.5 and 37°C.Fundação para a Ciência e a Tecnologia (FCT)SpringerUniversidade do MinhoAbrunhosa, LuísVenâncio, Armando2007-122007-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/7472eng"Biotechnology Letters." ISSN 0141-5492. 29:12 (Dez. 2007) p. 1909-1914.0141-549210.1007/s10529-007-9479-217653510info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T04:34:38Zoai:repositorium.sdum.uminho.pt:1822/7472Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T04:34:38Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Isolation and purification of an enzyme hydrolyzing ochratoxin A from aspergillus niger
title Isolation and purification of an enzyme hydrolyzing ochratoxin A from aspergillus niger
spellingShingle Isolation and purification of an enzyme hydrolyzing ochratoxin A from aspergillus niger
Abrunhosa, Luís
Aspergillus niger
Carboxypeptidase
Degradation
Ochratoxin A
Ochratoxin α
Science & Technology
title_short Isolation and purification of an enzyme hydrolyzing ochratoxin A from aspergillus niger
title_full Isolation and purification of an enzyme hydrolyzing ochratoxin A from aspergillus niger
title_fullStr Isolation and purification of an enzyme hydrolyzing ochratoxin A from aspergillus niger
title_full_unstemmed Isolation and purification of an enzyme hydrolyzing ochratoxin A from aspergillus niger
title_sort Isolation and purification of an enzyme hydrolyzing ochratoxin A from aspergillus niger
author Abrunhosa, Luís
author_facet Abrunhosa, Luís
Venâncio, Armando
author_role author
author2 Venâncio, Armando
author2_role author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Abrunhosa, Luís
Venâncio, Armando
dc.subject.por.fl_str_mv Aspergillus niger
Carboxypeptidase
Degradation
Ochratoxin A
Ochratoxin α
Science & Technology
topic Aspergillus niger
Carboxypeptidase
Degradation
Ochratoxin A
Ochratoxin α
Science & Technology
description Ochratoxin A is a mycotoxin produced by several Aspergillus and some Penicillium species which may be present in food and feed products. It can be enzymatically hydrolyzed into ochratoxin α and l-β-phenylalanine, thereby decreasing its toxicity. The ochratoxin A degradation capacity of Aspergillus niger is well known and here we report the isolation and purification of a novel enzyme from A. niger that hydrolyzes this mycotoxin. A wheat germ medium supplemented with ochratoxin A was used to produce the enzyme, which was purified from culture filtrate by acetone precipitation and anion exchange chromatography. An overall purification of 2.5-fold with a recovery of 68% and a final specific activity of 36 U/mg was obtained. The enzyme is a metalloenzyme as it was inhibited at 10 mM EDTA, whereas PMSF had no effect. The ochratoxin A hydrolytic enzyme presented a V max of 0.44 μM/min and a K m of 0.5 mM when the reaction was carried out at pH 7.5 and 37°C.
publishDate 2007
dc.date.none.fl_str_mv 2007-12
2007-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/7472
url http://hdl.handle.net/1822/7472
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Biotechnology Letters." ISSN 0141-5492. 29:12 (Dez. 2007) p. 1909-1914.
0141-5492
10.1007/s10529-007-9479-2
17653510
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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