Inorganic Hg toxicity in plants: a comparison of different genotoxic parameters

Detalhes bibliográficos
Autor(a) principal: Azevedo, Raquel
Data de Publicação: 2018
Outros Autores: Rodriguez, Eleazar, Mendes, Rafael José, Mariz-Ponte, Nuno, Sario, Sara, Lopes, José Carlos, Ferreira de Oliveira, José Miguel P, Santos, Conceição
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/37321
Resumo: Inorganic Mercury (Hg) contamination persists an environmental problem, but its cyto- and genotoxicity in plants remains yet unquantified. To determine the extent of Hg-induced cyto- and genotoxicity, and assess most sensitive endpoints in plants, Pisum sativum L. seedlings were exposed for 14 days to different HgCl2 concentrations up to 100 μM. Shoots and roots from hydroponic exposure presented growth impairment and/or morphological disorders for doses >1 μM, being the roots more sensitive. Plant growth, ploidy changes, clastogenicity (HPCV), cell cycle dynamics (G1-S-G2), Comet-tail moment (TM), Comet-TD, Mitotic-index (MI) and cell proliferation index (CPI) were used to evaluate Hg-induced cyto/genotoxicity. Both leaf and root DNA-ploidy levels, assessed by flow cytometry (FCM), remained unaltered after exposure. Root cell cycle impairment occurred at lower doses (≥1 μM) than structural DNA damages (≥10 μM). Cytostatic effects depended on the Hg concentration, with delays during S-phase at lower doses, and arrests at G1 at higher ones. This arrest was paralleled with decreases of both mitotic index (MI) and cell proliferation index (CPI). DNA fragmentation, assessed by the Comet assay parameters of TD and TM, could be visualized for conditions ≥10 μM, while FCM-clastogenic parameter (FPCV) and micronuclei (MNC) were only altered in roots exposed to 100 μM. We demonstrate that inorganic-Hg induced cytostaticity is detectable even at 1 μM (a value found in contaminated sites), while structural DNA breaks/damage are only visualized in plants at concentrations ≥10 μM. We also demonstrate that among the different techniques tested for cyto- and genotoxicity, TD and TM Comet endpoints were more sensitive than FPCV or MNC. Regarding cytostatic effects, cell cycle analysis by FCM, including the difference in % cell cycle phases and CPI were more sensitive than MI or MNC frequency. Our data contribute to better understand Hg cyto- and genotoxicity in plants and to understand the information and sensitivity provided by each of the genotoxic techniques used.
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spelling Inorganic Hg toxicity in plants: a comparison of different genotoxic parametersMercuryCell cycleComet assayFlow cytometryMicronucleiPisum sativumInorganic Mercury (Hg) contamination persists an environmental problem, but its cyto- and genotoxicity in plants remains yet unquantified. To determine the extent of Hg-induced cyto- and genotoxicity, and assess most sensitive endpoints in plants, Pisum sativum L. seedlings were exposed for 14 days to different HgCl2 concentrations up to 100 μM. Shoots and roots from hydroponic exposure presented growth impairment and/or morphological disorders for doses >1 μM, being the roots more sensitive. Plant growth, ploidy changes, clastogenicity (HPCV), cell cycle dynamics (G1-S-G2), Comet-tail moment (TM), Comet-TD, Mitotic-index (MI) and cell proliferation index (CPI) were used to evaluate Hg-induced cyto/genotoxicity. Both leaf and root DNA-ploidy levels, assessed by flow cytometry (FCM), remained unaltered after exposure. Root cell cycle impairment occurred at lower doses (≥1 μM) than structural DNA damages (≥10 μM). Cytostatic effects depended on the Hg concentration, with delays during S-phase at lower doses, and arrests at G1 at higher ones. This arrest was paralleled with decreases of both mitotic index (MI) and cell proliferation index (CPI). DNA fragmentation, assessed by the Comet assay parameters of TD and TM, could be visualized for conditions ≥10 μM, while FCM-clastogenic parameter (FPCV) and micronuclei (MNC) were only altered in roots exposed to 100 μM. We demonstrate that inorganic-Hg induced cytostaticity is detectable even at 1 μM (a value found in contaminated sites), while structural DNA breaks/damage are only visualized in plants at concentrations ≥10 μM. We also demonstrate that among the different techniques tested for cyto- and genotoxicity, TD and TM Comet endpoints were more sensitive than FPCV or MNC. Regarding cytostatic effects, cell cycle analysis by FCM, including the difference in % cell cycle phases and CPI were more sensitive than MI or MNC frequency. Our data contribute to better understand Hg cyto- and genotoxicity in plants and to understand the information and sensitivity provided by each of the genotoxic techniques used.Elsevier2023-04-24T12:12:48Z2018-04-01T00:00:00Z2018-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/37321eng0981-942810.1016/j.plaphy.2018.02.015Azevedo, RaquelRodriguez, EleazarMendes, Rafael JoséMariz-Ponte, NunoSario, SaraLopes, José CarlosFerreira de Oliveira, José Miguel PSantos, Conceiçãoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:12:03Zoai:ria.ua.pt:10773/37321Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:07:57.053880Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Inorganic Hg toxicity in plants: a comparison of different genotoxic parameters
title Inorganic Hg toxicity in plants: a comparison of different genotoxic parameters
spellingShingle Inorganic Hg toxicity in plants: a comparison of different genotoxic parameters
Azevedo, Raquel
Mercury
Cell cycle
Comet assay
Flow cytometry
Micronuclei
Pisum sativum
title_short Inorganic Hg toxicity in plants: a comparison of different genotoxic parameters
title_full Inorganic Hg toxicity in plants: a comparison of different genotoxic parameters
title_fullStr Inorganic Hg toxicity in plants: a comparison of different genotoxic parameters
title_full_unstemmed Inorganic Hg toxicity in plants: a comparison of different genotoxic parameters
title_sort Inorganic Hg toxicity in plants: a comparison of different genotoxic parameters
author Azevedo, Raquel
author_facet Azevedo, Raquel
Rodriguez, Eleazar
Mendes, Rafael José
Mariz-Ponte, Nuno
Sario, Sara
Lopes, José Carlos
Ferreira de Oliveira, José Miguel P
Santos, Conceição
author_role author
author2 Rodriguez, Eleazar
Mendes, Rafael José
Mariz-Ponte, Nuno
Sario, Sara
Lopes, José Carlos
Ferreira de Oliveira, José Miguel P
Santos, Conceição
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Azevedo, Raquel
Rodriguez, Eleazar
Mendes, Rafael José
Mariz-Ponte, Nuno
Sario, Sara
Lopes, José Carlos
Ferreira de Oliveira, José Miguel P
Santos, Conceição
dc.subject.por.fl_str_mv Mercury
Cell cycle
Comet assay
Flow cytometry
Micronuclei
Pisum sativum
topic Mercury
Cell cycle
Comet assay
Flow cytometry
Micronuclei
Pisum sativum
description Inorganic Mercury (Hg) contamination persists an environmental problem, but its cyto- and genotoxicity in plants remains yet unquantified. To determine the extent of Hg-induced cyto- and genotoxicity, and assess most sensitive endpoints in plants, Pisum sativum L. seedlings were exposed for 14 days to different HgCl2 concentrations up to 100 μM. Shoots and roots from hydroponic exposure presented growth impairment and/or morphological disorders for doses >1 μM, being the roots more sensitive. Plant growth, ploidy changes, clastogenicity (HPCV), cell cycle dynamics (G1-S-G2), Comet-tail moment (TM), Comet-TD, Mitotic-index (MI) and cell proliferation index (CPI) were used to evaluate Hg-induced cyto/genotoxicity. Both leaf and root DNA-ploidy levels, assessed by flow cytometry (FCM), remained unaltered after exposure. Root cell cycle impairment occurred at lower doses (≥1 μM) than structural DNA damages (≥10 μM). Cytostatic effects depended on the Hg concentration, with delays during S-phase at lower doses, and arrests at G1 at higher ones. This arrest was paralleled with decreases of both mitotic index (MI) and cell proliferation index (CPI). DNA fragmentation, assessed by the Comet assay parameters of TD and TM, could be visualized for conditions ≥10 μM, while FCM-clastogenic parameter (FPCV) and micronuclei (MNC) were only altered in roots exposed to 100 μM. We demonstrate that inorganic-Hg induced cytostaticity is detectable even at 1 μM (a value found in contaminated sites), while structural DNA breaks/damage are only visualized in plants at concentrations ≥10 μM. We also demonstrate that among the different techniques tested for cyto- and genotoxicity, TD and TM Comet endpoints were more sensitive than FPCV or MNC. Regarding cytostatic effects, cell cycle analysis by FCM, including the difference in % cell cycle phases and CPI were more sensitive than MI or MNC frequency. Our data contribute to better understand Hg cyto- and genotoxicity in plants and to understand the information and sensitivity provided by each of the genotoxic techniques used.
publishDate 2018
dc.date.none.fl_str_mv 2018-04-01T00:00:00Z
2018-04
2023-04-24T12:12:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/37321
url http://hdl.handle.net/10773/37321
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0981-9428
10.1016/j.plaphy.2018.02.015
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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