Isolation and amino acid sequencing by MALDI-TOF-MS/MS of a novel antimicrobial anionic peptide from the skin secretion of Osteocephalus taurinus (Anura, Hylidae)

Detalhes bibliográficos
Autor(a) principal: Costa,Túlio O. G.
Data de Publicação: 2012
Outros Autores: Almeida,Richardson A., Melo,Jorge T., Koolen,Hector H. F., Silva,Felipe M. A. da, Leite,José Roberto S. A., Prates,Maura V., Bloch Jr.,Carlos, Pinto,Angelo C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012001200002
Resumo: By means of chromatographic techniques, a novel anionic peptide, named otacidin, was isolated from the skin secretions of tree Brazilian anurans Osteocephalus taurinus. The de novo sequencing of the amino acids was performed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS/MS) analyses. The antimicrobial potential of this novel metabolite was evaluated against Escherichia coli and Pseudomonas aeruginosa, being observed minimum inhibitory concentration (MICs) of 37.4 and 78.4 mM, respectively. This work is just the second report of an antimicrobial anionic peptide from an amphibian.
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spelling Isolation and amino acid sequencing by MALDI-TOF-MS/MS of a novel antimicrobial anionic peptide from the skin secretion of Osteocephalus taurinus (Anura, Hylidae)anionic peptideantimicrobial peptideOsteocephalus taurinusskin secretionBy means of chromatographic techniques, a novel anionic peptide, named otacidin, was isolated from the skin secretions of tree Brazilian anurans Osteocephalus taurinus. The de novo sequencing of the amino acids was performed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS/MS) analyses. The antimicrobial potential of this novel metabolite was evaluated against Escherichia coli and Pseudomonas aeruginosa, being observed minimum inhibitory concentration (MICs) of 37.4 and 78.4 mM, respectively. This work is just the second report of an antimicrobial anionic peptide from an amphibian.Sociedade Brasileira de Química2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012001200002Journal of the Brazilian Chemical Society v.23 n.12 2012reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532012001200002info:eu-repo/semantics/openAccessCosta,Túlio O. G.Almeida,Richardson A.Melo,Jorge T.Koolen,Hector H. F.Silva,Felipe M. A. daLeite,José Roberto S. A.Prates,Maura V.Bloch Jr.,CarlosPinto,Angelo C.eng2013-02-08T00:00:00Zoai:scielo:S0103-50532012001200002Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2013-02-08T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Isolation and amino acid sequencing by MALDI-TOF-MS/MS of a novel antimicrobial anionic peptide from the skin secretion of Osteocephalus taurinus (Anura, Hylidae)
title Isolation and amino acid sequencing by MALDI-TOF-MS/MS of a novel antimicrobial anionic peptide from the skin secretion of Osteocephalus taurinus (Anura, Hylidae)
spellingShingle Isolation and amino acid sequencing by MALDI-TOF-MS/MS of a novel antimicrobial anionic peptide from the skin secretion of Osteocephalus taurinus (Anura, Hylidae)
Costa,Túlio O. G.
anionic peptide
antimicrobial peptide
Osteocephalus taurinus
skin secretion
title_short Isolation and amino acid sequencing by MALDI-TOF-MS/MS of a novel antimicrobial anionic peptide from the skin secretion of Osteocephalus taurinus (Anura, Hylidae)
title_full Isolation and amino acid sequencing by MALDI-TOF-MS/MS of a novel antimicrobial anionic peptide from the skin secretion of Osteocephalus taurinus (Anura, Hylidae)
title_fullStr Isolation and amino acid sequencing by MALDI-TOF-MS/MS of a novel antimicrobial anionic peptide from the skin secretion of Osteocephalus taurinus (Anura, Hylidae)
title_full_unstemmed Isolation and amino acid sequencing by MALDI-TOF-MS/MS of a novel antimicrobial anionic peptide from the skin secretion of Osteocephalus taurinus (Anura, Hylidae)
title_sort Isolation and amino acid sequencing by MALDI-TOF-MS/MS of a novel antimicrobial anionic peptide from the skin secretion of Osteocephalus taurinus (Anura, Hylidae)
author Costa,Túlio O. G.
author_facet Costa,Túlio O. G.
Almeida,Richardson A.
Melo,Jorge T.
Koolen,Hector H. F.
Silva,Felipe M. A. da
Leite,José Roberto S. A.
Prates,Maura V.
Bloch Jr.,Carlos
Pinto,Angelo C.
author_role author
author2 Almeida,Richardson A.
Melo,Jorge T.
Koolen,Hector H. F.
Silva,Felipe M. A. da
Leite,José Roberto S. A.
Prates,Maura V.
Bloch Jr.,Carlos
Pinto,Angelo C.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa,Túlio O. G.
Almeida,Richardson A.
Melo,Jorge T.
Koolen,Hector H. F.
Silva,Felipe M. A. da
Leite,José Roberto S. A.
Prates,Maura V.
Bloch Jr.,Carlos
Pinto,Angelo C.
dc.subject.por.fl_str_mv anionic peptide
antimicrobial peptide
Osteocephalus taurinus
skin secretion
topic anionic peptide
antimicrobial peptide
Osteocephalus taurinus
skin secretion
description By means of chromatographic techniques, a novel anionic peptide, named otacidin, was isolated from the skin secretions of tree Brazilian anurans Osteocephalus taurinus. The de novo sequencing of the amino acids was performed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS/MS) analyses. The antimicrobial potential of this novel metabolite was evaluated against Escherichia coli and Pseudomonas aeruginosa, being observed minimum inhibitory concentration (MICs) of 37.4 and 78.4 mM, respectively. This work is just the second report of an antimicrobial anionic peptide from an amphibian.
publishDate 2012
dc.date.none.fl_str_mv 2012-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012001200002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012001200002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532012001200002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.23 n.12 2012
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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