Evaluation of Listeria monocytogenes biofilm formation: comparison between persistent and sporadic strains

Detalhes bibliográficos
Autor(a) principal: Miranda, Tomás Albuquerque
Data de Publicação: 2017
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/13896
Resumo: Mestrado em Engenharia Alimentar - Qualidade e Segurança Alimentar - Instituto Superior de Agronomia
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spelling Evaluation of Listeria monocytogenes biofilm formation: comparison between persistent and sporadic strainsbiofilmsListeria monocytogenessusceptibilitydesinfectantevaluationMestrado em Engenharia Alimentar - Qualidade e Segurança Alimentar - Instituto Superior de AgronomiaL. monocytogenes has proven to be persistent in several food industries, with different environmental conditions, including nutritional availability, raising the important question: which is the major factor associated with persistence and can this be related to disinfectant susceptibility. A group of 10 persistent and 10 non persistent strains from different origins were selected and tested for biofilm forming ability using microtiter plate assay crystal violet (CV) and stainless steel coupon (SSC) methods at 25 ºC and 11 ºC for 48 h and 7 days, respectively. Susceptibility of a subset of 12 of these strains was tested by log reduction of cell enumeration from stainless steel coupons exposed to two commercial hydrogen peroxide acidic based disinfectants (P3 OXONIA and MIDIA SAN 315). Results showed no tendency between persistence traits and biofilm forming ability, even though significant differences (p<0.05) were found between both incubation temperatures. Simulation of food industry conditions (cold and nutritional stress) with biofilms on SSC also showed no relation between persistence traits and high biofilm forming ability. Susceptibility results corroborate these results by showing no significant differences in disinfectant susceptibility between persistent and non persistent strains, although P3 OXONIA with acetic and peracetic acid proved to be more effective than MIDIA SAN 315 with citric acidISA/ULBrito, Maria Luísa de CastroCunha, Paula Cristina Branco CabritaRepositório da Universidade de LisboaMiranda, Tomás Albuquerque2017-07-10T11:13:31Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/13896engMiranda, T.A. - Evaluation of Listeria monocytogenes biofilm formation: comparison between persistent and sporadic strains. Lisboa: ISA, 2017, 39 p.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:43:59Zoai:www.repository.utl.pt:10400.5/13896Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:59:48.496753Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evaluation of Listeria monocytogenes biofilm formation: comparison between persistent and sporadic strains
title Evaluation of Listeria monocytogenes biofilm formation: comparison between persistent and sporadic strains
spellingShingle Evaluation of Listeria monocytogenes biofilm formation: comparison between persistent and sporadic strains
Miranda, Tomás Albuquerque
biofilms
Listeria monocytogenes
susceptibility
desinfectant
evaluation
title_short Evaluation of Listeria monocytogenes biofilm formation: comparison between persistent and sporadic strains
title_full Evaluation of Listeria monocytogenes biofilm formation: comparison between persistent and sporadic strains
title_fullStr Evaluation of Listeria monocytogenes biofilm formation: comparison between persistent and sporadic strains
title_full_unstemmed Evaluation of Listeria monocytogenes biofilm formation: comparison between persistent and sporadic strains
title_sort Evaluation of Listeria monocytogenes biofilm formation: comparison between persistent and sporadic strains
author Miranda, Tomás Albuquerque
author_facet Miranda, Tomás Albuquerque
author_role author
dc.contributor.none.fl_str_mv Brito, Maria Luísa de Castro
Cunha, Paula Cristina Branco Cabrita
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Miranda, Tomás Albuquerque
dc.subject.por.fl_str_mv biofilms
Listeria monocytogenes
susceptibility
desinfectant
evaluation
topic biofilms
Listeria monocytogenes
susceptibility
desinfectant
evaluation
description Mestrado em Engenharia Alimentar - Qualidade e Segurança Alimentar - Instituto Superior de Agronomia
publishDate 2017
dc.date.none.fl_str_mv 2017-07-10T11:13:31Z
2017
2017-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/13896
url http://hdl.handle.net/10400.5/13896
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Miranda, T.A. - Evaluation of Listeria monocytogenes biofilm formation: comparison between persistent and sporadic strains. Lisboa: ISA, 2017, 39 p.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ISA/UL
publisher.none.fl_str_mv ISA/UL
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799131084669059072

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