Croton argyrophyllus Kunth essential oil-loaded solid lipid nanoparticles: evaluation of release profile, antioxidant activity and cytotoxicity in a neuroblastoma cell line
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/67053 |
Resumo: | The essential oil from Croton argyrophyllus Kunth is known for its antiproliferative, anti-inflammatory, antinociceptive, and anticancer activities, and is recognized as a source of phytochemicals for potential use in pharmaceutic and food sectors. Solid lipid nanoparticles (SLN) have been produced to load Croton argyrophyllus (CA) Kunth essential oil (CAEO) and its antioxidant properties evaluated in vitro as a new approach for the treatment of neurodegenerative diseases. Cetyl palmitate SLN loading CAEO (CAEO-SLN) with a mean particle size of 201.4 ± 2.3 nm (polydispersity index 0.211) have been produced by hot high-pressure homogenisation. The release of the oil followed the Korsmeyers-Peppas model. The risk of lipid peroxidation has been determined by applying the production of thiobarbituric acid-reactive substances (TBARS) standard assay. The antioxidant activity was determined by the capacity of the antioxidants existing in CAEO to scavenge the stable radical DPPH•. The cytotoxicity of CA Kunth essential oil-loaded SLN (CAEO-SLN) was evaluated in a human cell line SH-SY5Y (derived from human neuroblastoma) by determining the reduction of the yellow dye 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT). Both free essential oil (fEO) and loaded essential oil (CAEO-SLN) were demonstrated to inhibit the Fenton reaction. CAEO-SLN showed DPPH• radical scavenging capacity. The loading of the oil into cetyl palmitate SLN reduced the risk of cytotoxicity. |
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Croton argyrophyllus Kunth essential oil-loaded solid lipid nanoparticles: evaluation of release profile, antioxidant activity and cytotoxicity in a neuroblastoma cell lineantioxidantcytotoxicessential oilCroton argyrophyllus Kunthneurological disordersScience & TechnologyThe essential oil from Croton argyrophyllus Kunth is known for its antiproliferative, anti-inflammatory, antinociceptive, and anticancer activities, and is recognized as a source of phytochemicals for potential use in pharmaceutic and food sectors. Solid lipid nanoparticles (SLN) have been produced to load Croton argyrophyllus (CA) Kunth essential oil (CAEO) and its antioxidant properties evaluated in vitro as a new approach for the treatment of neurodegenerative diseases. Cetyl palmitate SLN loading CAEO (CAEO-SLN) with a mean particle size of 201.4 ± 2.3 nm (polydispersity index 0.211) have been produced by hot high-pressure homogenisation. The release of the oil followed the Korsmeyers-Peppas model. The risk of lipid peroxidation has been determined by applying the production of thiobarbituric acid-reactive substances (TBARS) standard assay. The antioxidant activity was determined by the capacity of the antioxidants existing in CAEO to scavenge the stable radical DPPH•. The cytotoxicity of CA Kunth essential oil-loaded SLN (CAEO-SLN) was evaluated in a human cell line SH-SY5Y (derived from human neuroblastoma) by determining the reduction of the yellow dye 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT). Both free essential oil (fEO) and loaded essential oil (CAEO-SLN) were demonstrated to inhibit the Fenton reaction. CAEO-SLN showed DPPH• radical scavenging capacity. The loading of the oil into cetyl palmitate SLN reduced the risk of cytotoxicity.This research was supported by the Coordenação Aperfeiçoamento de Pessoal de Nivel Superior (CAPES),Fundação de Amparo à Pesquisa do Estado de Sergipe (FAPITEC) CHAMADA MS/CNPq/FAPITEC/SE/SESN◦06/2018—PROGRAMA DE PESQUISA PARA O SUS: GESTÃO COMPARTILHADA EM SAÚDE—PPSUSSERGIPE 2017/2018, and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). This research was also supported through the projects M-ERA-NET/0004/2015-PAIRED and UIDB/04469/2020 (strategic fund),receiving support from the Portuguese Science and Technology Foundation, Ministry of Science and Education(FCT/MEC) through national funds, and co-financed by FEDER, under the Partnership Agreement PT2020.The authors acknowledge the support of the research project: Nutraceutica come supporto nutrizionale nelpaziente oncologico, CUP: B83D18000140007.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoSouto, Eliana B.Severino, PatriciaMarques, ConradoAndrade, Luciana N.Durazzo, AlessandraLucarini, MassimoAtanasov, Atanas G.El Maimouni, SoukainaNovellino, EttoreSantini, Antonello20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/67053engSouto, Eliana; Severino, Patricia; Marques, Conrado; Andrade, Luciana N.; Durazzo, Alessandra; Lucarini, Massimo; Atanasov, Atanas G.; El Maimouni, Soukaina; Novellino, Ettore; Santini, Antonello, Croton argyrophyllus Kunth essential oil-loaded solid lipid nanoparticles: evaluation of release profile, antioxidant activity and cytotoxicity in a neuroblastoma cell line. Sustainability, 12(18), 7697, 20202071-105010.3390/su12187697https://www.mdpi.com/journal/sustainabilityinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:19:21Zoai:repositorium.sdum.uminho.pt:1822/67053Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:12:16.701200Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Croton argyrophyllus Kunth essential oil-loaded solid lipid nanoparticles: evaluation of release profile, antioxidant activity and cytotoxicity in a neuroblastoma cell line |
title |
Croton argyrophyllus Kunth essential oil-loaded solid lipid nanoparticles: evaluation of release profile, antioxidant activity and cytotoxicity in a neuroblastoma cell line |
spellingShingle |
Croton argyrophyllus Kunth essential oil-loaded solid lipid nanoparticles: evaluation of release profile, antioxidant activity and cytotoxicity in a neuroblastoma cell line Souto, Eliana B. antioxidant cytotoxic essential oil Croton argyrophyllus Kunth neurological disorders Science & Technology |
title_short |
Croton argyrophyllus Kunth essential oil-loaded solid lipid nanoparticles: evaluation of release profile, antioxidant activity and cytotoxicity in a neuroblastoma cell line |
title_full |
Croton argyrophyllus Kunth essential oil-loaded solid lipid nanoparticles: evaluation of release profile, antioxidant activity and cytotoxicity in a neuroblastoma cell line |
title_fullStr |
Croton argyrophyllus Kunth essential oil-loaded solid lipid nanoparticles: evaluation of release profile, antioxidant activity and cytotoxicity in a neuroblastoma cell line |
title_full_unstemmed |
Croton argyrophyllus Kunth essential oil-loaded solid lipid nanoparticles: evaluation of release profile, antioxidant activity and cytotoxicity in a neuroblastoma cell line |
title_sort |
Croton argyrophyllus Kunth essential oil-loaded solid lipid nanoparticles: evaluation of release profile, antioxidant activity and cytotoxicity in a neuroblastoma cell line |
author |
Souto, Eliana B. |
author_facet |
Souto, Eliana B. Severino, Patricia Marques, Conrado Andrade, Luciana N. Durazzo, Alessandra Lucarini, Massimo Atanasov, Atanas G. El Maimouni, Soukaina Novellino, Ettore Santini, Antonello |
author_role |
author |
author2 |
Severino, Patricia Marques, Conrado Andrade, Luciana N. Durazzo, Alessandra Lucarini, Massimo Atanasov, Atanas G. El Maimouni, Soukaina Novellino, Ettore Santini, Antonello |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Souto, Eliana B. Severino, Patricia Marques, Conrado Andrade, Luciana N. Durazzo, Alessandra Lucarini, Massimo Atanasov, Atanas G. El Maimouni, Soukaina Novellino, Ettore Santini, Antonello |
dc.subject.por.fl_str_mv |
antioxidant cytotoxic essential oil Croton argyrophyllus Kunth neurological disorders Science & Technology |
topic |
antioxidant cytotoxic essential oil Croton argyrophyllus Kunth neurological disorders Science & Technology |
description |
The essential oil from Croton argyrophyllus Kunth is known for its antiproliferative, anti-inflammatory, antinociceptive, and anticancer activities, and is recognized as a source of phytochemicals for potential use in pharmaceutic and food sectors. Solid lipid nanoparticles (SLN) have been produced to load Croton argyrophyllus (CA) Kunth essential oil (CAEO) and its antioxidant properties evaluated in vitro as a new approach for the treatment of neurodegenerative diseases. Cetyl palmitate SLN loading CAEO (CAEO-SLN) with a mean particle size of 201.4 ± 2.3 nm (polydispersity index 0.211) have been produced by hot high-pressure homogenisation. The release of the oil followed the Korsmeyers-Peppas model. The risk of lipid peroxidation has been determined by applying the production of thiobarbituric acid-reactive substances (TBARS) standard assay. The antioxidant activity was determined by the capacity of the antioxidants existing in CAEO to scavenge the stable radical DPPH•. The cytotoxicity of CA Kunth essential oil-loaded SLN (CAEO-SLN) was evaluated in a human cell line SH-SY5Y (derived from human neuroblastoma) by determining the reduction of the yellow dye 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT). Both free essential oil (fEO) and loaded essential oil (CAEO-SLN) were demonstrated to inhibit the Fenton reaction. CAEO-SLN showed DPPH• radical scavenging capacity. The loading of the oil into cetyl palmitate SLN reduced the risk of cytotoxicity. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/67053 |
url |
http://hdl.handle.net/1822/67053 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Souto, Eliana; Severino, Patricia; Marques, Conrado; Andrade, Luciana N.; Durazzo, Alessandra; Lucarini, Massimo; Atanasov, Atanas G.; El Maimouni, Soukaina; Novellino, Ettore; Santini, Antonello, Croton argyrophyllus Kunth essential oil-loaded solid lipid nanoparticles: evaluation of release profile, antioxidant activity and cytotoxicity in a neuroblastoma cell line. Sustainability, 12(18), 7697, 2020 2071-1050 10.3390/su12187697 https://www.mdpi.com/journal/sustainability |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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