Analyzing Oct4 conserved domains in lower vertebrates

Detalhes bibliográficos
Autor(a) principal: Sousa, Cátia Alexandra Ferreira de
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/2955
Resumo: Dissertação de mest., Engenharia Biológica, Faculdade de Engenharia e de Recursos Naturais, Univ. do Algarve, 2008
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spelling Analyzing Oct4 conserved domains in lower vertebratesVertebradosOct4DNADissertação de mest., Engenharia Biológica, Faculdade de Engenharia e de Recursos Naturais, Univ. do Algarve, 2008Oct-4 is a POU-V domain transcription factor which regulates pluripotency in mammals and expressed in ES cells and germ cells, and was believed to be a gene unique to mammals. Recently, it has been demonstrated to be present in the genomes of Xenopus, zebrafish, sturgeon and axolotl. It has been shown that Oct-4 has three transactivation domains, (N), POU and (C), with DNA binding mediated through the POU domain. It was unknown how the activity of these domains has been retained through evolution and how they collectively function to control pluripotency. This study is new and is the first to analyse the functional conservation of these Oct-4 homologues, and their regulation molecular. Three different assays were developed to study Oct4 functionality: 1) A transactivation assay in which the function of Oct4 protein over-expression on a known Oct4-target sequence was assessed (by luciferase assay using the p6Wtk-luc reporter containing Oct4 binding sites); 2) A heterologous transactivation assay in which the function of specific Oct4 domains by linking them to Gal4-DNA binding domain was specifically assessed (DBD) (by luciferase assay using the pGal4-lux reporter). 3) The subcellular localization of Oct-4 homologues (generating two constructs; either full’ length or POU domain, fused to Green fluorescent protein (GFP). This study showed the coexistence of DBD conflicts with Oct4 resulting in a decrease on its transactivation capacity when compared to their native state. Oct-4 function generally conserved among species, with Xenopus Oct91 being the Oct-4 homologue with a transactivation function more similar to mouse Oct-4. Between (C) and (N) transactivation domains linked to DBD, the (C) domain was the one with more activity. The (C) domain is cell-type specific regulated by phosphorylation events, while the (N) domain suffers sumoylation. These two regulatory mechanisms are shared in all Oct-4 homologues. It was also possible to conclude that Oct-4 protein is nuclei transcribed. This project opens many possible studies in Oct-4 regulation.Johnson, AndrewTannahill, DavidSapientiaSousa, Cátia Alexandra Ferreira de2013-10-01T08:41:53Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.1/2955enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:14:00Zoai:sapientia.ualg.pt:10400.1/2955Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:56:39.771956Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Analyzing Oct4 conserved domains in lower vertebrates
title Analyzing Oct4 conserved domains in lower vertebrates
spellingShingle Analyzing Oct4 conserved domains in lower vertebrates
Sousa, Cátia Alexandra Ferreira de
Vertebrados
Oct4
DNA
title_short Analyzing Oct4 conserved domains in lower vertebrates
title_full Analyzing Oct4 conserved domains in lower vertebrates
title_fullStr Analyzing Oct4 conserved domains in lower vertebrates
title_full_unstemmed Analyzing Oct4 conserved domains in lower vertebrates
title_sort Analyzing Oct4 conserved domains in lower vertebrates
author Sousa, Cátia Alexandra Ferreira de
author_facet Sousa, Cátia Alexandra Ferreira de
author_role author
dc.contributor.none.fl_str_mv Johnson, Andrew
Tannahill, David
Sapientia
dc.contributor.author.fl_str_mv Sousa, Cátia Alexandra Ferreira de
dc.subject.por.fl_str_mv Vertebrados
Oct4
DNA
topic Vertebrados
Oct4
DNA
description Dissertação de mest., Engenharia Biológica, Faculdade de Engenharia e de Recursos Naturais, Univ. do Algarve, 2008
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
2013-10-01T08:41:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/2955
url http://hdl.handle.net/10400.1/2955
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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