Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/100671 https://doi.org/10.3390/polym13050793 |
Resumo: | Gene therapy could be simply defined as a strategy for the introduction of a functional copy of desired genes in patients, to correct some specific mutation and potentially treat the respective disorder. However, this straightforward definition hides very complex processes related to the design and preparation of the therapeutic genes, as well as the development of suitable gene delivery systems. Within non-viral vectors, polymeric nanocarriers have offered an ideal platform to be applied as gene delivery systems. Concerning this, the main goal of the study was to do a systematic evaluation on the formulation of pDNA delivery systems based on the complexation of different sized plasmids with chitosan (CH) or polyethyleneimine (PEI) polymers to search for the best option regarding encapsulation efficiency, surface charge, size, and delivery ability. The cytotoxicity and the transfection efficiency of these systems were accessed and, for the best p53 encoding pDNA nanosystems, the ability to promote protein expression was also evaluated. Overall, it was showed that CH polyplexes are more efficient on transfection when compared with the PEI polyplexes, resulting in higher P53 protein expression. Cells transfected with CH/p53-pDNA polyplexes presented an increase of around 54.2% on P53 expression, while the transfection with the PEI/p53-pDNA polyplexes resulted in a 32% increase. |
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Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulationchitosangene therapypolyethyleneiminepolyplexestransgene expressionGene therapy could be simply defined as a strategy for the introduction of a functional copy of desired genes in patients, to correct some specific mutation and potentially treat the respective disorder. However, this straightforward definition hides very complex processes related to the design and preparation of the therapeutic genes, as well as the development of suitable gene delivery systems. Within non-viral vectors, polymeric nanocarriers have offered an ideal platform to be applied as gene delivery systems. Concerning this, the main goal of the study was to do a systematic evaluation on the formulation of pDNA delivery systems based on the complexation of different sized plasmids with chitosan (CH) or polyethyleneimine (PEI) polymers to search for the best option regarding encapsulation efficiency, surface charge, size, and delivery ability. The cytotoxicity and the transfection efficiency of these systems were accessed and, for the best p53 encoding pDNA nanosystems, the ability to promote protein expression was also evaluated. Overall, it was showed that CH polyplexes are more efficient on transfection when compared with the PEI polyplexes, resulting in higher P53 protein expression. Cells transfected with CH/p53-pDNA polyplexes presented an increase of around 54.2% on P53 expression, while the transfection with the PEI/p53-pDNA polyplexes resulted in a 32% increase.2021-03-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/100671http://hdl.handle.net/10316/100671https://doi.org/10.3390/polym13050793eng2073-4360Valente, J. F. A.Pereira, P.Sousa, A.Queiroz, J. A.Sousa, F.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-07-08T20:38:25Zoai:estudogeral.uc.pt:10316/100671Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:18:00.499079Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation |
title |
Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation |
spellingShingle |
Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation Valente, J. F. A. chitosan gene therapy polyethyleneimine polyplexes transgene expression |
title_short |
Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation |
title_full |
Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation |
title_fullStr |
Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation |
title_full_unstemmed |
Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation |
title_sort |
Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation |
author |
Valente, J. F. A. |
author_facet |
Valente, J. F. A. Pereira, P. Sousa, A. Queiroz, J. A. Sousa, F. |
author_role |
author |
author2 |
Pereira, P. Sousa, A. Queiroz, J. A. Sousa, F. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Valente, J. F. A. Pereira, P. Sousa, A. Queiroz, J. A. Sousa, F. |
dc.subject.por.fl_str_mv |
chitosan gene therapy polyethyleneimine polyplexes transgene expression |
topic |
chitosan gene therapy polyethyleneimine polyplexes transgene expression |
description |
Gene therapy could be simply defined as a strategy for the introduction of a functional copy of desired genes in patients, to correct some specific mutation and potentially treat the respective disorder. However, this straightforward definition hides very complex processes related to the design and preparation of the therapeutic genes, as well as the development of suitable gene delivery systems. Within non-viral vectors, polymeric nanocarriers have offered an ideal platform to be applied as gene delivery systems. Concerning this, the main goal of the study was to do a systematic evaluation on the formulation of pDNA delivery systems based on the complexation of different sized plasmids with chitosan (CH) or polyethyleneimine (PEI) polymers to search for the best option regarding encapsulation efficiency, surface charge, size, and delivery ability. The cytotoxicity and the transfection efficiency of these systems were accessed and, for the best p53 encoding pDNA nanosystems, the ability to promote protein expression was also evaluated. Overall, it was showed that CH polyplexes are more efficient on transfection when compared with the PEI polyplexes, resulting in higher P53 protein expression. Cells transfected with CH/p53-pDNA polyplexes presented an increase of around 54.2% on P53 expression, while the transfection with the PEI/p53-pDNA polyplexes resulted in a 32% increase. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-03-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/100671 http://hdl.handle.net/10316/100671 https://doi.org/10.3390/polym13050793 |
url |
http://hdl.handle.net/10316/100671 https://doi.org/10.3390/polym13050793 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2073-4360 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799134075581104128 |