Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coli
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/109753 https://doi.org/10.3390/molecules18032712 |
Resumo: | Several families of photosensitizers are currently being scrutinized for antimicrobial photodynamic therapy applications. Differences in physical and photochemical properties can lead to different localization patterns as well as differences in singlet oxygen production and decay when the photosensitizers are taken up by bacterial cells. We have examined the production and fate of singlet oxygen in Escherichia coli upon photosensitization with three structurally-different cationic photosensitizers, namely New Methylene Blue N (NMB), a member of the phenothiazine family, ACS268, a hydrophobic porphyrin with a single cationic alkyl chain, and zinc(II)-tetramethyltetrapyridinoporphyrazinium salt, a phthalocyanine-like photosensitizer with four positive charges on the macrocycle core. The kinetics of singlet oxygen production and decay indicate different localization for the three photosensitizers, whereby NMB appears to localize in an aqueous-like microenvironment, whereas ACS268 localizes in an oxygen-shielded site, highly reactive towards singlet oxygen. The tetracationic zinc(II) tetrapyridinoporphyrazine is extensively aggregated in the bacteria and fails to produce any detectable singlet oxygen. |
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Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coliantimicrobial photodynamic therapycationic photosensitizersE. colikineticsphotodynamic inactivationsinglet oxygentime-resolved near-IR spectroscopyAnti-Infective AgentsEscherichia coliIndolesIsoindolesKineticsPhotosensitizing AgentsPorphyrinsSinglet OxygenPhotochemotherapySeveral families of photosensitizers are currently being scrutinized for antimicrobial photodynamic therapy applications. Differences in physical and photochemical properties can lead to different localization patterns as well as differences in singlet oxygen production and decay when the photosensitizers are taken up by bacterial cells. We have examined the production and fate of singlet oxygen in Escherichia coli upon photosensitization with three structurally-different cationic photosensitizers, namely New Methylene Blue N (NMB), a member of the phenothiazine family, ACS268, a hydrophobic porphyrin with a single cationic alkyl chain, and zinc(II)-tetramethyltetrapyridinoporphyrazinium salt, a phthalocyanine-like photosensitizer with four positive charges on the macrocycle core. The kinetics of singlet oxygen production and decay indicate different localization for the three photosensitizers, whereby NMB appears to localize in an aqueous-like microenvironment, whereas ACS268 localizes in an oxygen-shielded site, highly reactive towards singlet oxygen. The tetracationic zinc(II) tetrapyridinoporphyrazine is extensively aggregated in the bacteria and fails to produce any detectable singlet oxygen.MDPI2013-02-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/109753http://hdl.handle.net/10316/109753https://doi.org/10.3390/molecules18032712eng1420-3049Ragàs, XavierHe, XinAgut, MontserratRoxo-Rosa, MónicaRocha-Gonsalves, AntónioSerra, ArménioNonell, Santiinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-25T09:57:07Zoai:estudogeral.uc.pt:10316/109753Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:25:54.207797Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coli |
title |
Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coli |
spellingShingle |
Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coli Ragàs, Xavier antimicrobial photodynamic therapy cationic photosensitizers E. coli kinetics photodynamic inactivation singlet oxygen time-resolved near-IR spectroscopy Anti-Infective Agents Escherichia coli Indoles Isoindoles Kinetics Photosensitizing Agents Porphyrins Singlet Oxygen Photochemotherapy |
title_short |
Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coli |
title_full |
Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coli |
title_fullStr |
Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coli |
title_full_unstemmed |
Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coli |
title_sort |
Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coli |
author |
Ragàs, Xavier |
author_facet |
Ragàs, Xavier He, Xin Agut, Montserrat Roxo-Rosa, Mónica Rocha-Gonsalves, António Serra, Arménio Nonell, Santi |
author_role |
author |
author2 |
He, Xin Agut, Montserrat Roxo-Rosa, Mónica Rocha-Gonsalves, António Serra, Arménio Nonell, Santi |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Ragàs, Xavier He, Xin Agut, Montserrat Roxo-Rosa, Mónica Rocha-Gonsalves, António Serra, Arménio Nonell, Santi |
dc.subject.por.fl_str_mv |
antimicrobial photodynamic therapy cationic photosensitizers E. coli kinetics photodynamic inactivation singlet oxygen time-resolved near-IR spectroscopy Anti-Infective Agents Escherichia coli Indoles Isoindoles Kinetics Photosensitizing Agents Porphyrins Singlet Oxygen Photochemotherapy |
topic |
antimicrobial photodynamic therapy cationic photosensitizers E. coli kinetics photodynamic inactivation singlet oxygen time-resolved near-IR spectroscopy Anti-Infective Agents Escherichia coli Indoles Isoindoles Kinetics Photosensitizing Agents Porphyrins Singlet Oxygen Photochemotherapy |
description |
Several families of photosensitizers are currently being scrutinized for antimicrobial photodynamic therapy applications. Differences in physical and photochemical properties can lead to different localization patterns as well as differences in singlet oxygen production and decay when the photosensitizers are taken up by bacterial cells. We have examined the production and fate of singlet oxygen in Escherichia coli upon photosensitization with three structurally-different cationic photosensitizers, namely New Methylene Blue N (NMB), a member of the phenothiazine family, ACS268, a hydrophobic porphyrin with a single cationic alkyl chain, and zinc(II)-tetramethyltetrapyridinoporphyrazinium salt, a phthalocyanine-like photosensitizer with four positive charges on the macrocycle core. The kinetics of singlet oxygen production and decay indicate different localization for the three photosensitizers, whereby NMB appears to localize in an aqueous-like microenvironment, whereas ACS268 localizes in an oxygen-shielded site, highly reactive towards singlet oxygen. The tetracationic zinc(II) tetrapyridinoporphyrazine is extensively aggregated in the bacteria and fails to produce any detectable singlet oxygen. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02-28 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/109753 http://hdl.handle.net/10316/109753 https://doi.org/10.3390/molecules18032712 |
url |
http://hdl.handle.net/10316/109753 https://doi.org/10.3390/molecules18032712 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1420-3049 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799134140818259968 |