Overexpression of wild-type IL-7Rα promotes T-cell acute lymphoblastic leukemia/lymphoma
| Autor(a) principal: | |
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| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/128467 |
Resumo: | U117573801 MR/P011225/1 ERC CoG-648455 KiKa 2010-082 |
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Overexpression of wild-type IL-7Rα promotes T-cell acute lymphoblastic leukemia/lymphomaBiochemistryImmunologyHematologyCell BiologySDG 3 - Good Health and Well-beingU117573801 MR/P011225/1 ERC CoG-648455 KiKa 2010-082Tight regulation of IL-7Rα expression is essential for normal T-cell development. IL-7Rα gain-of-function mutations are known drivers of T-cell acute lymphoblastic leukemia (T-ALL). Although a subset of patients with T-ALL display high IL7R messenger RNA levels and cases with IL7R gains have been reported, the impact of IL-7Rα overexpression, rather than mutational activation, during leukemogenesis remains unclear. In this study, overexpressed IL-7Rα in tetracycline-inducible Il7r transgenic and Rosa26 IL7R knockin mice drove potential thymocyte self-renewal, and thymus hyperplasia related to increased proliferation of T-cell precursors, which subsequently infiltrated lymph nodes, spleen, and bone marrow, ultimately leading to fatal leukemia. The tumors mimicked key features of human T-ALL, including heterogeneity in immunophenotype and genetic subtype between cases, frequent hyperactivation of the PI3K/Akt pathway paralleled by downregulation of p27Kip1 and upregulation of Bcl-2, and gene expression signatures evidencing activation of JAK/STAT, PI3K/Akt/mTOR and Notch signaling. Notably, we also found that established tumors may no longer require high levels of IL-7R expression upon secondary transplantation and progressed in the absence of IL-7, but remain sensitive to inhibitors of IL-7R–mediated signaling ruxolitinib (Jak1), AZD1208 (Pim), dactolisib (PI3K/mTOR), palbociclib (Cdk4/6), and venetoclax (Bcl-2). The relevance of these findings for human disease are highlighted by the fact that samples from patients with T-ALL with high wild-type IL7R expression display a transcriptional signature resembling that of IL-7–stimulated pro-T cells and, critically, of IL7R-mutant cases of T-ALL. Overall, our study demonstrates that high expression of IL-7Rα can promote T-cell tumorigenesis, even in the absence of IL-7Rα mutational activation.DCV - Departamento de Ciências da VidaUCIBIO - Applied Molecular Biosciences UnitRUNSilva, Ana TeresaAlmeida, Afonso R. M.Cachucho, AnaNeto, João L.Demeyer, Sofiede Matos, MafaldaHogan, TheaLi, YunleiMeijerink, JulesCools, JanGrosso, Ana RitaSeddon, BenedictBarata, João T.2021-11-29T23:40:00Z2021-09-232021-09-23T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article13application/pdfhttp://hdl.handle.net/10362/128467eng0006-4971PURE: 35107125https://doi.org/10.1182/blood.2019000553info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:08:03Zoai:run.unl.pt:10362/128467Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:46:18.927661Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Overexpression of wild-type IL-7Rα promotes T-cell acute lymphoblastic leukemia/lymphoma |
| title |
Overexpression of wild-type IL-7Rα promotes T-cell acute lymphoblastic leukemia/lymphoma |
| spellingShingle |
Overexpression of wild-type IL-7Rα promotes T-cell acute lymphoblastic leukemia/lymphoma Silva, Ana Teresa Biochemistry Immunology Hematology Cell Biology SDG 3 - Good Health and Well-being |
| title_short |
Overexpression of wild-type IL-7Rα promotes T-cell acute lymphoblastic leukemia/lymphoma |
| title_full |
Overexpression of wild-type IL-7Rα promotes T-cell acute lymphoblastic leukemia/lymphoma |
| title_fullStr |
Overexpression of wild-type IL-7Rα promotes T-cell acute lymphoblastic leukemia/lymphoma |
| title_full_unstemmed |
Overexpression of wild-type IL-7Rα promotes T-cell acute lymphoblastic leukemia/lymphoma |
| title_sort |
Overexpression of wild-type IL-7Rα promotes T-cell acute lymphoblastic leukemia/lymphoma |
| author |
Silva, Ana Teresa |
| author_facet |
Silva, Ana Teresa Almeida, Afonso R. M. Cachucho, Ana Neto, João L. Demeyer, Sofie de Matos, Mafalda Hogan, Thea Li, Yunlei Meijerink, Jules Cools, Jan Grosso, Ana Rita Seddon, Benedict Barata, João T. |
| author_role |
author |
| author2 |
Almeida, Afonso R. M. Cachucho, Ana Neto, João L. Demeyer, Sofie de Matos, Mafalda Hogan, Thea Li, Yunlei Meijerink, Jules Cools, Jan Grosso, Ana Rita Seddon, Benedict Barata, João T. |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
DCV - Departamento de Ciências da Vida UCIBIO - Applied Molecular Biosciences Unit RUN |
| dc.contributor.author.fl_str_mv |
Silva, Ana Teresa Almeida, Afonso R. M. Cachucho, Ana Neto, João L. Demeyer, Sofie de Matos, Mafalda Hogan, Thea Li, Yunlei Meijerink, Jules Cools, Jan Grosso, Ana Rita Seddon, Benedict Barata, João T. |
| dc.subject.por.fl_str_mv |
Biochemistry Immunology Hematology Cell Biology SDG 3 - Good Health and Well-being |
| topic |
Biochemistry Immunology Hematology Cell Biology SDG 3 - Good Health and Well-being |
| description |
U117573801 MR/P011225/1 ERC CoG-648455 KiKa 2010-082 |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-11-29T23:40:00Z 2021-09-23 2021-09-23T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/128467 |
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http://hdl.handle.net/10362/128467 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
0006-4971 PURE: 35107125 https://doi.org/10.1182/blood.2019000553 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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13 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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