Obtention of plant peroxidase and its potential for the decolorization of the reactive dye Remazol Turquoise G 133%

• Autor(a) principal: Silva, Maria Cristina
• Data de Publicação: 2012
• Outros Autores: Torres, Juliana A., Corrêa, Angelita D., Bernardes, Allana Maria, Amorim, M. T. Pessoa de, Santos, Custódio Donizete
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/21916
• topic: Decolorization; Environmental biocatalysis; Peroxidase; Textile dyes; Turnip; Science & Technology

Peroxidases can be used in the decolorization process. There is a growing interest for new sources of this enzyme and for obtaining economically viable processes. In this work, a low-cost vegetable peroxidase extraction process is proposed; the resulting enzyme is characterized to determine its optimum pH, temperature, and stability conditions, and it is then applied in the decolorization of reactive dye Remazol Turquoise G 133%. The turnip peroxidase (TP) was utilized as an enzymatic source. This enzyme exhibited maximum activity at pH 7.0, and it was active in the temperature range of 30 to 50 °C, which favors its use in industrial processes. Acetone was the most efficient solvent to induce precipitation. The removal of Remazol Turquoise G 133% was 56.0% complete after 50 min, while 41.0% of the same dye was removed with the commercial horseradish peroxidase enzyme in 50 min. TP presents potential as a viable alternative in the decolorization of textile wastewaters.