Decolorization of the phthalocyanine dye reactive blue 21 by turnip peroxidase and assessment of its oxidation products

Detalhes bibliográficos
Autor(a) principal: Silva, Maria Cristina
Data de Publicação: 2012
Outros Autores: Corrêa, Angelita D., Amorim, M. T. Pessoa de, Parpot, Pier, Torres, Juliana A., Chagas, Pricila Maria Batista
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/21897
Resumo: Peroxidases can be used in decolorization processes and the treatment of textiles effluents. This study evaluates the potential of the turnip peroxidase enzyme in the decolorization of the phthalocyanine textile dye Reactive Blue 21 (RB21). Some factors such as pH, the amount of H2O2 and the enzyme were evaluated in order to determine the optimum conditions for the enzyme performance. The reaction products formed during the decolorization of the RB21 dye were analyzed by high-performance liquid chromatography–mass spectrometry coupling (LC–ESI/MS). LC–ESI/MS analysis showed that the decolorization of the dye RB21 by turnip peroxidase is due to the breaking up of the chromatogenous system. The tests for toxicity towards lettuce seeds showed an increase of the toxicity after enzymatic treatment of the dye. This study verifies the viability of the use of the turnip peroxidase enzyme in the biodegradation of textile dyes.
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spelling Decolorization of the phthalocyanine dye reactive blue 21 by turnip peroxidase and assessment of its oxidation productsEnvironmental biocatalysisLC–ESI/MSTurnip peroxidaseDecolorizationTextile dyesScience & TechnologyPeroxidases can be used in decolorization processes and the treatment of textiles effluents. This study evaluates the potential of the turnip peroxidase enzyme in the decolorization of the phthalocyanine textile dye Reactive Blue 21 (RB21). Some factors such as pH, the amount of H2O2 and the enzyme were evaluated in order to determine the optimum conditions for the enzyme performance. The reaction products formed during the decolorization of the RB21 dye were analyzed by high-performance liquid chromatography–mass spectrometry coupling (LC–ESI/MS). LC–ESI/MS analysis showed that the decolorization of the dye RB21 by turnip peroxidase is due to the breaking up of the chromatogenous system. The tests for toxicity towards lettuce seeds showed an increase of the toxicity after enzymatic treatment of the dye. This study verifies the viability of the use of the turnip peroxidase enzyme in the biodegradation of textile dyes.FAPEMIGCNPqCAPESElsevierUniversidade do MinhoSilva, Maria CristinaCorrêa, Angelita D.Amorim, M. T. Pessoa deParpot, PierTorres, Juliana A.Chagas, Pricila Maria Batista20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/21897eng1381-117710.1016/j.molcatb.2011.12.006info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:36:53Zoai:repositorium.sdum.uminho.pt:1822/21897Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:33:04.803308Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Decolorization of the phthalocyanine dye reactive blue 21 by turnip peroxidase and assessment of its oxidation products
title Decolorization of the phthalocyanine dye reactive blue 21 by turnip peroxidase and assessment of its oxidation products
spellingShingle Decolorization of the phthalocyanine dye reactive blue 21 by turnip peroxidase and assessment of its oxidation products
Silva, Maria Cristina
Environmental biocatalysis
LC–ESI/MS
Turnip peroxidase
Decolorization
Textile dyes
Science & Technology
title_short Decolorization of the phthalocyanine dye reactive blue 21 by turnip peroxidase and assessment of its oxidation products
title_full Decolorization of the phthalocyanine dye reactive blue 21 by turnip peroxidase and assessment of its oxidation products
title_fullStr Decolorization of the phthalocyanine dye reactive blue 21 by turnip peroxidase and assessment of its oxidation products
title_full_unstemmed Decolorization of the phthalocyanine dye reactive blue 21 by turnip peroxidase and assessment of its oxidation products
title_sort Decolorization of the phthalocyanine dye reactive blue 21 by turnip peroxidase and assessment of its oxidation products
author Silva, Maria Cristina
author_facet Silva, Maria Cristina
Corrêa, Angelita D.
Amorim, M. T. Pessoa de
Parpot, Pier
Torres, Juliana A.
Chagas, Pricila Maria Batista
author_role author
author2 Corrêa, Angelita D.
Amorim, M. T. Pessoa de
Parpot, Pier
Torres, Juliana A.
Chagas, Pricila Maria Batista
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva, Maria Cristina
Corrêa, Angelita D.
Amorim, M. T. Pessoa de
Parpot, Pier
Torres, Juliana A.
Chagas, Pricila Maria Batista
dc.subject.por.fl_str_mv Environmental biocatalysis
LC–ESI/MS
Turnip peroxidase
Decolorization
Textile dyes
Science & Technology
topic Environmental biocatalysis
LC–ESI/MS
Turnip peroxidase
Decolorization
Textile dyes
Science & Technology
description Peroxidases can be used in decolorization processes and the treatment of textiles effluents. This study evaluates the potential of the turnip peroxidase enzyme in the decolorization of the phthalocyanine textile dye Reactive Blue 21 (RB21). Some factors such as pH, the amount of H2O2 and the enzyme were evaluated in order to determine the optimum conditions for the enzyme performance. The reaction products formed during the decolorization of the RB21 dye were analyzed by high-performance liquid chromatography–mass spectrometry coupling (LC–ESI/MS). LC–ESI/MS analysis showed that the decolorization of the dye RB21 by turnip peroxidase is due to the breaking up of the chromatogenous system. The tests for toxicity towards lettuce seeds showed an increase of the toxicity after enzymatic treatment of the dye. This study verifies the viability of the use of the turnip peroxidase enzyme in the biodegradation of textile dyes.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/21897
url http://hdl.handle.net/1822/21897
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1381-1177
10.1016/j.molcatb.2011.12.006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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