Cytogenomics and cytological approaches disclose the occurrence of diploid nuclei throughout the life cycles of Pucciniales fungi and reveal a unique heterokaryotic system

Detalhes bibliográficos
Autor(a) principal: Carvalho, Rita Maria Domingues
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/23532
Resumo: Mestrado em Biologia dos Recursos Vegetais / Instituto Superior de Agronomia. Universidade de Lisboa
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spelling Cytogenomics and cytological approaches disclose the occurrence of diploid nuclei throughout the life cycles of Pucciniales fungi and reveal a unique heterokaryotic systemflow cytometry (FCM)fluorescence in situ hybridization (FISH)rust fungiheterokaryonMestrado em Biologia dos Recursos Vegetais / Instituto Superior de Agronomia. Universidade de LisboaPucciniales (Basidiomycota) is an order containing thousands of species, all of them biotrophic plant pathogens, establishing highly specific interactions with their hosts, frequently undergoing complex life cycles. In contrast with neighbouring orders, Pucciniales fungi present large genomes. Whereas diploid nuclei would be expectable only in basidia, in the present work the occurrence of haploid and diploid nuclei in a wide range of Pucciniales species across their life cycles is reported not only using FCM but also with FISH using rDNA probes, even if it was also detected in one fungus of the order Platygloeales the phenomenon is likely to be circumscribed to Pucciniales. Although all rust fungi are dikaryotic during the most part of their life cycles, the fungus Ctss presents nuclei with distinct genome sizes (the Twin Peaks phenomenon), and this trait allows the separation of the two nuclei populations by FACS. This constitutes an unprecedented tool for the study of the heterokaryon in Pucciniales. In the present work, a RNA-seq analyses was done on these nuclei populations (A and B) separately to evaluate the transcripts, allowing to conclude that 41% are common in both nuclei populations and ~33% are exclusive of A and 25% of B nuclei population. Jointly with the amplification of some DNA fragments of genes of interest by PCR it was proved that the populations of nuclei A and B effectively constitute the heterokaryon of Ctss. The commercial globalization, the environmental changes, and the appearance of new rust fungi in new locations enhance the need of detailed knowledge on rust-host interactions. Future studies will allow to clarify the nature of both the Twin Peaks and the haploid/diploid phenomena, which certainly may be applied to better understand such pathosystems.ISA/ULAzinheira, HelenaTalhinhas, PedroRepositório da Universidade de LisboaCarvalho, Rita Maria Domingues2024-02-01T01:30:37Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/23532TID:203085400engCarvalho, R.M.D. - Cytogenomics and cytological approaches disclose the occurrence of diploid nuclei throughout the life cycles of Pucciniales fungi and reveal a unique heterokaryotic system. Lisboa: ISA, 2021, 67 p.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-04T01:32:12Zoai:www.repository.utl.pt:10400.5/23532Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:07:44.977365Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cytogenomics and cytological approaches disclose the occurrence of diploid nuclei throughout the life cycles of Pucciniales fungi and reveal a unique heterokaryotic system
title Cytogenomics and cytological approaches disclose the occurrence of diploid nuclei throughout the life cycles of Pucciniales fungi and reveal a unique heterokaryotic system
spellingShingle Cytogenomics and cytological approaches disclose the occurrence of diploid nuclei throughout the life cycles of Pucciniales fungi and reveal a unique heterokaryotic system
Carvalho, Rita Maria Domingues
flow cytometry (FCM)
fluorescence in situ hybridization (FISH)
rust fungi
heterokaryon
title_short Cytogenomics and cytological approaches disclose the occurrence of diploid nuclei throughout the life cycles of Pucciniales fungi and reveal a unique heterokaryotic system
title_full Cytogenomics and cytological approaches disclose the occurrence of diploid nuclei throughout the life cycles of Pucciniales fungi and reveal a unique heterokaryotic system
title_fullStr Cytogenomics and cytological approaches disclose the occurrence of diploid nuclei throughout the life cycles of Pucciniales fungi and reveal a unique heterokaryotic system
title_full_unstemmed Cytogenomics and cytological approaches disclose the occurrence of diploid nuclei throughout the life cycles of Pucciniales fungi and reveal a unique heterokaryotic system
title_sort Cytogenomics and cytological approaches disclose the occurrence of diploid nuclei throughout the life cycles of Pucciniales fungi and reveal a unique heterokaryotic system
author Carvalho, Rita Maria Domingues
author_facet Carvalho, Rita Maria Domingues
author_role author
dc.contributor.none.fl_str_mv Azinheira, Helena
Talhinhas, Pedro
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Carvalho, Rita Maria Domingues
dc.subject.por.fl_str_mv flow cytometry (FCM)
fluorescence in situ hybridization (FISH)
rust fungi
heterokaryon
topic flow cytometry (FCM)
fluorescence in situ hybridization (FISH)
rust fungi
heterokaryon
description Mestrado em Biologia dos Recursos Vegetais / Instituto Superior de Agronomia. Universidade de Lisboa
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
2024-02-01T01:30:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/23532
TID:203085400
url http://hdl.handle.net/10400.5/23532
identifier_str_mv TID:203085400
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Carvalho, R.M.D. - Cytogenomics and cytological approaches disclose the occurrence of diploid nuclei throughout the life cycles of Pucciniales fungi and reveal a unique heterokaryotic system. Lisboa: ISA, 2021, 67 p.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ISA/UL
publisher.none.fl_str_mv ISA/UL
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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