Genomic analysis of two novel bacteriophages infecting Acinetobacter beijerinckii and halotolerans species

Detalhes bibliográficos
Autor(a) principal: Gomes, Marta Teresa Silva
Data de Publicação: 2023
Outros Autores: Domingues, Rita Alexandra Carpinteiro, Turner, Dann, Oliveira, Hugo Alexandre Mendes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/83078
Resumo: Bacteriophages are the most diverse genetic entities on Earth. In this study, two novel bacteriophages, nACB1 (Podoviridae morphotype) and nACB2 (Myoviridae morphotype), which infect Acinetobacter beijerinckii and Acinetobacter halotolerans, respectively, were isolated from sewage samples. The genome sequences of nACB1 and nACB2 revealed that their genome sizes were 80,310 bp and 136,560 bp, respectively. Comparative analysis showed that both genomes are novel members of the Schitoviridae and the Ackermannviridae families, sharing ≤ 40% overall nucleotide identities with any other phages. Interestingly, among other genetic features, nACB1 encoded a very large RNA polymerase, while nACB2 displayed three putative depolymerases (two capsular depolymerases and one capsular esterase) encoded in tandem. This is the first report of phages infecting A. halotolerans and beijerinckii human pathogenic species. The findings regarding these two phages will allow us to further explore phage—Acinetobacter interactions and the genetic evolution for this group of phages.
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spelling Genomic analysis of two novel bacteriophages infecting Acinetobacter beijerinckii and halotolerans speciesAcinetobacterBacteriophagesComparative genomicsBioinformaticsScience & TechnologyBacteriophages are the most diverse genetic entities on Earth. In this study, two novel bacteriophages, nACB1 (Podoviridae morphotype) and nACB2 (Myoviridae morphotype), which infect Acinetobacter beijerinckii and Acinetobacter halotolerans, respectively, were isolated from sewage samples. The genome sequences of nACB1 and nACB2 revealed that their genome sizes were 80,310 bp and 136,560 bp, respectively. Comparative analysis showed that both genomes are novel members of the Schitoviridae and the Ackermannviridae families, sharing ≤ 40% overall nucleotide identities with any other phages. Interestingly, among other genetic features, nACB1 encoded a very large RNA polymerase, while nACB2 displayed three putative depolymerases (two capsular depolymerases and one capsular esterase) encoded in tandem. This is the first report of phages infecting A. halotolerans and beijerinckii human pathogenic species. The findings regarding these two phages will allow us to further explore phage—Acinetobacter interactions and the genetic evolution for this group of phages.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit, and by LABBELS—Associate Laboratory in Biotechnology, Bioengineering and Microelectromechnical Systems, LA/P/0029/2020.info:eu-repo/semantics/publishedVersionMultidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoGomes, Marta Teresa SilvaDomingues, Rita Alexandra CarpinteiroTurner, DannOliveira, Hugo Alexandre Mendes2023-02-262023-02-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/83078engGomes, Marta; Domingues, Rita; Turner, Dann; Oliveira, Hugo, Genomic analysis of two novel bacteriophages infecting Acinetobacter beijerinckii and halotolerans species. Viruses, 15(3), 643, 20231999-491510.3390/v1503064336992352643https://www.mdpi.com/1999-4915/15/3/643info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:47:36Zoai:repositorium.sdum.uminho.pt:1822/83078Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:45:40.020244Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genomic analysis of two novel bacteriophages infecting Acinetobacter beijerinckii and halotolerans species
title Genomic analysis of two novel bacteriophages infecting Acinetobacter beijerinckii and halotolerans species
spellingShingle Genomic analysis of two novel bacteriophages infecting Acinetobacter beijerinckii and halotolerans species
Gomes, Marta Teresa Silva
Acinetobacter
Bacteriophages
Comparative genomics
Bioinformatics
Science & Technology
title_short Genomic analysis of two novel bacteriophages infecting Acinetobacter beijerinckii and halotolerans species
title_full Genomic analysis of two novel bacteriophages infecting Acinetobacter beijerinckii and halotolerans species
title_fullStr Genomic analysis of two novel bacteriophages infecting Acinetobacter beijerinckii and halotolerans species
title_full_unstemmed Genomic analysis of two novel bacteriophages infecting Acinetobacter beijerinckii and halotolerans species
title_sort Genomic analysis of two novel bacteriophages infecting Acinetobacter beijerinckii and halotolerans species
author Gomes, Marta Teresa Silva
author_facet Gomes, Marta Teresa Silva
Domingues, Rita Alexandra Carpinteiro
Turner, Dann
Oliveira, Hugo Alexandre Mendes
author_role author
author2 Domingues, Rita Alexandra Carpinteiro
Turner, Dann
Oliveira, Hugo Alexandre Mendes
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gomes, Marta Teresa Silva
Domingues, Rita Alexandra Carpinteiro
Turner, Dann
Oliveira, Hugo Alexandre Mendes
dc.subject.por.fl_str_mv Acinetobacter
Bacteriophages
Comparative genomics
Bioinformatics
Science & Technology
topic Acinetobacter
Bacteriophages
Comparative genomics
Bioinformatics
Science & Technology
description Bacteriophages are the most diverse genetic entities on Earth. In this study, two novel bacteriophages, nACB1 (Podoviridae morphotype) and nACB2 (Myoviridae morphotype), which infect Acinetobacter beijerinckii and Acinetobacter halotolerans, respectively, were isolated from sewage samples. The genome sequences of nACB1 and nACB2 revealed that their genome sizes were 80,310 bp and 136,560 bp, respectively. Comparative analysis showed that both genomes are novel members of the Schitoviridae and the Ackermannviridae families, sharing ≤ 40% overall nucleotide identities with any other phages. Interestingly, among other genetic features, nACB1 encoded a very large RNA polymerase, while nACB2 displayed three putative depolymerases (two capsular depolymerases and one capsular esterase) encoded in tandem. This is the first report of phages infecting A. halotolerans and beijerinckii human pathogenic species. The findings regarding these two phages will allow us to further explore phage—Acinetobacter interactions and the genetic evolution for this group of phages.
publishDate 2023
dc.date.none.fl_str_mv 2023-02-26
2023-02-26T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/83078
url https://hdl.handle.net/1822/83078
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gomes, Marta; Domingues, Rita; Turner, Dann; Oliveira, Hugo, Genomic analysis of two novel bacteriophages infecting Acinetobacter beijerinckii and halotolerans species. Viruses, 15(3), 643, 2023
1999-4915
10.3390/v15030643
36992352
643
https://www.mdpi.com/1999-4915/15/3/643
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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