Trimethylamine increases intestinal fatty acid absorption

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Catarina
Data de Publicação: 2023
Outros Autores: Ismael, Shámila, Castela, Inês, Barreiros-Mota, Inês, Almeida, Maria João, Santos, Gilberto Maia, Calhau, Conceição, Rocha, Júlio César, Faria, Ana, Araújo, João R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/161169
Resumo: Funding Information: This work was supported by ERDF through the operation POCI-01-0145-ERDF-007746 funded by the Programa Operacional Competitividade e Internacionalização – COMPETE2020 and by FCT – Fundação para a Ciência e a Tecnologia, IP national support through CINTESIS, R&D Unit (UIDB/4255/2020), CHRC (UIDP/04923/2020 and UIDB/04923/2020), and through the projects PTDC/BAA-AGR/7419/2020, 2022.11716.BD, and 2020.06333.BD. Publisher Copyright: © 2023 The Author(s). Published by Cambridge University Press on behalf of The Nutrition Society.
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spelling Trimethylamine increases intestinal fatty acid absorptionin vitro studies in a Caco-2 cell culture systemCaco-2 cellsEnterocytesFatty acid absorptionTrimethylamineFood ScienceEndocrinology, Diabetes and MetabolismNutrition and DieteticsSDG 3 - Good Health and Well-beingFunding Information: This work was supported by ERDF through the operation POCI-01-0145-ERDF-007746 funded by the Programa Operacional Competitividade e Internacionalização – COMPETE2020 and by FCT – Fundação para a Ciência e a Tecnologia, IP national support through CINTESIS, R&D Unit (UIDB/4255/2020), CHRC (UIDP/04923/2020 and UIDB/04923/2020), and through the projects PTDC/BAA-AGR/7419/2020, 2022.11716.BD, and 2020.06333.BD. Publisher Copyright: © 2023 The Author(s). Published by Cambridge University Press on behalf of The Nutrition Society.Although elevated blood levels of trimethylamine N-oxide (TMAO) have been associated with atherosclerosis development in humans, the role of its gut microbiota-derived precursor, TMA, in this process has not been yet deciphered. Taking this into account, and the fact that increased intestinal fatty acid absorption contributes to atherosclerosis onset and progression, this study aimed to evaluate the effect of TMA on fatty acid absorption in a cell line that mimics human enterocytes. Caco-2 cells were treated with TMA 250 M for 24 h. Fatty acid absorption was assessed by measuring the apical-to-basolateral transport and the intracellular levels of BODIPY-C12, a fluorescently labelled fatty acid analogue. Gene expression of the main intestinal fatty acid transporters was evaluated by real-time quantitative reverse transcription PCR. Compared to control conditions, TMA increased, in a time-dependent manner and by 20-50%, the apical-to-basolateral transport and intracellular levels of BODIPY-C12 fatty acid in Caco-2 cells. Fatty acid transport protein 4 (FATP4) and fatty acid translocase (FAT)/CD36 gene expression were not stimulated by TMA, suggesting that TMA-induced increase in fatty acid transport may be mediated by an increase in FAT/CD36 and/or FATP4 activity and/or fatty acid passive transport. This study demonstrated that TMA increases the intestinal absorption of fatty acids. Future studies are necessary to confirm if this may constitute a novel mechanism that partially explains the existing positive association between the consumption of a diet rich in TMA sources (e.g. red meat) and the increased risk of atherosclerotic diseases.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Comprehensive Health Research Centre (CHRC) - pólo NMSCentro de Investigação em Tecnologias e Serviços de Saúde (CINTESIS)RUNRodrigues, CatarinaIsmael, ShámilaCastela, InêsBarreiros-Mota, InêsAlmeida, Maria JoãoSantos, Gilberto MaiaCalhau, ConceiçãoRocha, Júlio CésarFaria, AnaAraújo, João R.2023-12-12T22:51:36Z2023-11-032023-11-03T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/161169eng2048-6790PURE: 78180476https://doi.org/10.1017/jns.2023.91info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:43:59Zoai:run.unl.pt:10362/161169Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:58:24.094580Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Trimethylamine increases intestinal fatty acid absorption
in vitro studies in a Caco-2 cell culture system
title Trimethylamine increases intestinal fatty acid absorption
spellingShingle Trimethylamine increases intestinal fatty acid absorption
Rodrigues, Catarina
Caco-2 cells
Enterocytes
Fatty acid absorption
Trimethylamine
Food Science
Endocrinology, Diabetes and Metabolism
Nutrition and Dietetics
SDG 3 - Good Health and Well-being
title_short Trimethylamine increases intestinal fatty acid absorption
title_full Trimethylamine increases intestinal fatty acid absorption
title_fullStr Trimethylamine increases intestinal fatty acid absorption
title_full_unstemmed Trimethylamine increases intestinal fatty acid absorption
title_sort Trimethylamine increases intestinal fatty acid absorption
author Rodrigues, Catarina
author_facet Rodrigues, Catarina
Ismael, Shámila
Castela, Inês
Barreiros-Mota, Inês
Almeida, Maria João
Santos, Gilberto Maia
Calhau, Conceição
Rocha, Júlio César
Faria, Ana
Araújo, João R.
author_role author
author2 Ismael, Shámila
Castela, Inês
Barreiros-Mota, Inês
Almeida, Maria João
Santos, Gilberto Maia
Calhau, Conceição
Rocha, Júlio César
Faria, Ana
Araújo, João R.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Comprehensive Health Research Centre (CHRC) - pólo NMS
Centro de Investigação em Tecnologias e Serviços de Saúde (CINTESIS)
RUN
dc.contributor.author.fl_str_mv Rodrigues, Catarina
Ismael, Shámila
Castela, Inês
Barreiros-Mota, Inês
Almeida, Maria João
Santos, Gilberto Maia
Calhau, Conceição
Rocha, Júlio César
Faria, Ana
Araújo, João R.
dc.subject.por.fl_str_mv Caco-2 cells
Enterocytes
Fatty acid absorption
Trimethylamine
Food Science
Endocrinology, Diabetes and Metabolism
Nutrition and Dietetics
SDG 3 - Good Health and Well-being
topic Caco-2 cells
Enterocytes
Fatty acid absorption
Trimethylamine
Food Science
Endocrinology, Diabetes and Metabolism
Nutrition and Dietetics
SDG 3 - Good Health and Well-being
description Funding Information: This work was supported by ERDF through the operation POCI-01-0145-ERDF-007746 funded by the Programa Operacional Competitividade e Internacionalização – COMPETE2020 and by FCT – Fundação para a Ciência e a Tecnologia, IP national support through CINTESIS, R&D Unit (UIDB/4255/2020), CHRC (UIDP/04923/2020 and UIDB/04923/2020), and through the projects PTDC/BAA-AGR/7419/2020, 2022.11716.BD, and 2020.06333.BD. Publisher Copyright: © 2023 The Author(s). Published by Cambridge University Press on behalf of The Nutrition Society.
publishDate 2023
dc.date.none.fl_str_mv 2023-12-12T22:51:36Z
2023-11-03
2023-11-03T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/161169
url http://hdl.handle.net/10362/161169
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2048-6790
PURE: 78180476
https://doi.org/10.1017/jns.2023.91
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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