IPA and its precursors differently modulate the proliferation, differentiation, and integrity of intestinal epithelial cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/158422 |
Resumo: | Funding Information: This work was supported by ERDF through the operation POCI-01-0145-ERDF-007746 funded by the Programa Operacional Competitividade e Internacionalização – COMPETE2020 and by FCT - Fundação para a Ciência e a Tecnologia, IP national support through CINTESIS, R&D Unit (UIDB/4255/2020), CHRC (UIDP/04923/2020 and UIDB/04923/2020), through the project reference PTDC/BAA-AGR/7419/2020 and the 2020.06333.BD. Publisher Copyright: © 2023 The Korean Nutrition Society and the Korean Society of Community Nutrition This is an Open Access article distributed. |
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IPA and its precursors differently modulate the proliferation, differentiation, and integrity of intestinal epithelial cellsCaco-2 cellscell proliferationindolespermeabilitytryptophanFood ScienceNutrition and DieteticsSDG 3 - Good Health and Well-beingFunding Information: This work was supported by ERDF through the operation POCI-01-0145-ERDF-007746 funded by the Programa Operacional Competitividade e Internacionalização – COMPETE2020 and by FCT - Fundação para a Ciência e a Tecnologia, IP national support through CINTESIS, R&D Unit (UIDB/4255/2020), CHRC (UIDP/04923/2020 and UIDB/04923/2020), through the project reference PTDC/BAA-AGR/7419/2020 and the 2020.06333.BD. Publisher Copyright: © 2023 The Korean Nutrition Society and the Korean Society of Community Nutrition This is an Open Access article distributed.BACKGROUND/OBJECTIVES: Indole-3-propionic acid (IPA) is a tryptophan-derived microbial metabolite that has been associated with protective effects against inflammatory and metabolic diseases. However, there is a lack of knowledge regarding the effects of IPA under physiological conditions and at the intestinal level. MATERIALS/METHODS: Human intestinal epithelial Caco-2 cells were treated for 2, 24, and/ or 72 h with IPA or its precursors – indole, tryptophan, and propionate – at 1, 10, 100, 250, or 500 μM to assess cell viability, integrity, differentiation, and proliferation. RESULTS: IPA induced cell proliferation and this effect was associated with a higher expression of extracellular signal-regulated kinase 2 (ERK2) and a lower expression of c-Jun. Although indole and propionate also induced cell proliferation, this involved ERK2 and c-Jun independent mechanisms. On the other hand, both tryptophan and propionate increased cell integrity and reduced the expression of claudin-1, whereas propionate decreased cell differentiation. CONCLUSIONS: In conclusion, these findings suggested that IPA and its precursors distinctly contribute to the proliferation, differentiation, and barrier function properties of human intestinal epithelial cells. Moreover, the pro-proliferative effect of IPA in intestinal epithelial cells was not explained by its precursors and is rather related to its whole chemical structure. Maintaining IPA at physiological levels, e.g., through IPA-producing commensal bacteria, may be important to preserve the integrity of the intestinal barrier and play an integral role in maintaining metabolic homeostasis.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNIsmael, ShámilaRodrigues, CatarinaSantos, Gilberto MaiaCastela, InêsBarreiros-Mota, InêsAlmeida, Maria JoãoCalhau, ConceiçãoFaria, AnaAraújo, João Ricardo2023-09-28T22:19:29Z2023-082023-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttp://hdl.handle.net/10362/158422eng1976-1457PURE: 70250654https://doi.org/10.4162/nrp.2023.17.4.616info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:40:47Zoai:run.unl.pt:10362/158422Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:07.666969Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
IPA and its precursors differently modulate the proliferation, differentiation, and integrity of intestinal epithelial cells |
| title |
IPA and its precursors differently modulate the proliferation, differentiation, and integrity of intestinal epithelial cells |
| spellingShingle |
IPA and its precursors differently modulate the proliferation, differentiation, and integrity of intestinal epithelial cells Ismael, Shámila Caco-2 cells cell proliferation indoles permeability tryptophan Food Science Nutrition and Dietetics SDG 3 - Good Health and Well-being |
| title_short |
IPA and its precursors differently modulate the proliferation, differentiation, and integrity of intestinal epithelial cells |
| title_full |
IPA and its precursors differently modulate the proliferation, differentiation, and integrity of intestinal epithelial cells |
| title_fullStr |
IPA and its precursors differently modulate the proliferation, differentiation, and integrity of intestinal epithelial cells |
| title_full_unstemmed |
IPA and its precursors differently modulate the proliferation, differentiation, and integrity of intestinal epithelial cells |
| title_sort |
IPA and its precursors differently modulate the proliferation, differentiation, and integrity of intestinal epithelial cells |
| author |
Ismael, Shámila |
| author_facet |
Ismael, Shámila Rodrigues, Catarina Santos, Gilberto Maia Castela, Inês Barreiros-Mota, Inês Almeida, Maria João Calhau, Conceição Faria, Ana Araújo, João Ricardo |
| author_role |
author |
| author2 |
Rodrigues, Catarina Santos, Gilberto Maia Castela, Inês Barreiros-Mota, Inês Almeida, Maria João Calhau, Conceição Faria, Ana Araújo, João Ricardo |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) RUN |
| dc.contributor.author.fl_str_mv |
Ismael, Shámila Rodrigues, Catarina Santos, Gilberto Maia Castela, Inês Barreiros-Mota, Inês Almeida, Maria João Calhau, Conceição Faria, Ana Araújo, João Ricardo |
| dc.subject.por.fl_str_mv |
Caco-2 cells cell proliferation indoles permeability tryptophan Food Science Nutrition and Dietetics SDG 3 - Good Health and Well-being |
| topic |
Caco-2 cells cell proliferation indoles permeability tryptophan Food Science Nutrition and Dietetics SDG 3 - Good Health and Well-being |
| description |
Funding Information: This work was supported by ERDF through the operation POCI-01-0145-ERDF-007746 funded by the Programa Operacional Competitividade e Internacionalização – COMPETE2020 and by FCT - Fundação para a Ciência e a Tecnologia, IP national support through CINTESIS, R&D Unit (UIDB/4255/2020), CHRC (UIDP/04923/2020 and UIDB/04923/2020), through the project reference PTDC/BAA-AGR/7419/2020 and the 2020.06333.BD. Publisher Copyright: © 2023 The Korean Nutrition Society and the Korean Society of Community Nutrition This is an Open Access article distributed. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-09-28T22:19:29Z 2023-08 2023-08-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/158422 |
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http://hdl.handle.net/10362/158422 |
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eng |
| language |
eng |
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1976-1457 PURE: 70250654 https://doi.org/10.4162/nrp.2023.17.4.616 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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15 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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