Antioxidant-Loaded Mucoadhesive Nanoparticles for Eye Drug Delivery: A New Strategy to Reduce Oxidative Stress

Detalhes bibliográficos
Autor(a) principal: Cordeiro, Sandra
Data de Publicação: 2021
Outros Autores: Silva, Beatriz, Martins, Ana, Ribeiro, Helena, Gonçalves, Lídia, Marto, Joana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/62048
Resumo: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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spelling Antioxidant-Loaded Mucoadhesive Nanoparticles for Eye Drug Delivery: A New Strategy to Reduce Oxidative Stressnanoparticleschitosanhyaluronic acidantioxidantmucoadhesiveeye drug delivery© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).There are several approaches to treat ocular diseases, which can be invasive or non-invasive. Within the non-invasive, new pharmaceutical strategies based on nanotechnology and mucoadhesive polymers are emerging methodologies, which aim to reach an efficient treatment of eye diseases. The aim of this work was the development of novel chitosan/hyaluronic acid nanoparticle systems with mucoadhesive properties, intended to encapsulate antioxidant molecules (e.g., crocin) aiming to reduce eye oxidative stress and, consequently, ocular disease. An ultraviolet (UV) absorber molecule, actinoquinol, was also added to the nanoparticles, to further decrease oxidative stress. The developed nanoparticles were characterized and the results showed a mean particle size lower than 400 nm, polydispersity index of 0.220 ± 0.034, positive zeta potential, and high yield. The nanoparticles were also characterized in terms of pH, osmolality, and viscosity. Mucoadhesion studies involving the determination of zeta potential, viscosity, and tackiness, showed a strong interaction between the nanoparticles and mucin. In vitro release studies using synthetic membranes in Franz diffusion cells were conducted to unravel the drug release kinetic profile. Ex vitro studies using pig eye scleras in Franz diffusion cells were performed to evaluate the permeation of the nanoparticles. Furthermore, in vitro assays using the ARPE-19 (adult retinal pigment epithelium) cell line showed that the nanoparticles can efficiently decrease oxidative stress and showed low cytotoxicity. Thus, the developed chitosan/hyaluronic acid nanoparticles are a promising system for the delivery of antioxidants to the eye, by increasing their residence time and controlling their delivery.This research was funded by the Fundação para a Ciência e Tecnologia, Portugal (UIDB/04138/2020 and UIDP/04138/2020 to iMed.ULisboa, CEECINST/00145/2018 to J.Marto and Principal Researcher grant CEECIND/03143/2017 (L.Gonçalves); FCT fellowship SFRH/BD/130476/2017).MDPIRepositório da Universidade de LisboaCordeiro, SandraSilva, BeatrizMartins, AnaRibeiro, HelenaGonçalves, LídiaMarto, Joana2024-01-23T16:46:02Z2021-02-192023-02-27T15:59:35Z2021-02-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/62048engCordeiro S, Silva B, Martins AM, Ribeiro HM, Gonçalves L, Marto J. Antioxidant-loaded mucoadhesive nanoparticles for eye drug delivery: a new strategy to reduce oxidative stress. Processes [Internet]. 19 de fevereiro de 2021;9(2):379. Disponível em: https://www.mdpi.com/2227-9717/9/2/379cv-prod-242094110.3390/pr9020379info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-29T01:19:34Zoai:repositorio.ul.pt:10451/62048Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:58:29.086083Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Antioxidant-Loaded Mucoadhesive Nanoparticles for Eye Drug Delivery: A New Strategy to Reduce Oxidative Stress
title Antioxidant-Loaded Mucoadhesive Nanoparticles for Eye Drug Delivery: A New Strategy to Reduce Oxidative Stress
spellingShingle Antioxidant-Loaded Mucoadhesive Nanoparticles for Eye Drug Delivery: A New Strategy to Reduce Oxidative Stress
Cordeiro, Sandra
nanoparticles
chitosan
hyaluronic acid
antioxidant
mucoadhesive
eye drug delivery
title_short Antioxidant-Loaded Mucoadhesive Nanoparticles for Eye Drug Delivery: A New Strategy to Reduce Oxidative Stress
title_full Antioxidant-Loaded Mucoadhesive Nanoparticles for Eye Drug Delivery: A New Strategy to Reduce Oxidative Stress
title_fullStr Antioxidant-Loaded Mucoadhesive Nanoparticles for Eye Drug Delivery: A New Strategy to Reduce Oxidative Stress
title_full_unstemmed Antioxidant-Loaded Mucoadhesive Nanoparticles for Eye Drug Delivery: A New Strategy to Reduce Oxidative Stress
title_sort Antioxidant-Loaded Mucoadhesive Nanoparticles for Eye Drug Delivery: A New Strategy to Reduce Oxidative Stress
author Cordeiro, Sandra
author_facet Cordeiro, Sandra
Silva, Beatriz
Martins, Ana
Ribeiro, Helena
Gonçalves, Lídia
Marto, Joana
author_role author
author2 Silva, Beatriz
Martins, Ana
Ribeiro, Helena
Gonçalves, Lídia
Marto, Joana
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Cordeiro, Sandra
Silva, Beatriz
Martins, Ana
Ribeiro, Helena
Gonçalves, Lídia
Marto, Joana
dc.subject.por.fl_str_mv nanoparticles
chitosan
hyaluronic acid
antioxidant
mucoadhesive
eye drug delivery
topic nanoparticles
chitosan
hyaluronic acid
antioxidant
mucoadhesive
eye drug delivery
description © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
publishDate 2021
dc.date.none.fl_str_mv 2021-02-19
2021-02-19T00:00:00Z
2023-02-27T15:59:35Z
2024-01-23T16:46:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/62048
url http://hdl.handle.net/10451/62048
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cordeiro S, Silva B, Martins AM, Ribeiro HM, Gonçalves L, Marto J. Antioxidant-loaded mucoadhesive nanoparticles for eye drug delivery: a new strategy to reduce oxidative stress. Processes [Internet]. 19 de fevereiro de 2021;9(2):379. Disponível em: https://www.mdpi.com/2227-9717/9/2/379
cv-prod-2420941
10.3390/pr9020379
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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