Fluorescent quantification of melanin

Detalhes bibliográficos
Autor(a) principal: Fernandes, Bruno Pacheco
Data de Publicação: 2016
Outros Autores: Matamá, Maria Teresa, Guimarães, Diana Isabel Pereira, Gomes, Andreia C, Cavaco-Paulo, Artur
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/43069
Resumo: Melanin quantification is reportedly performed by absorption spectroscopy, commonly at 405 nm. Here, we propose the implementation of fluorescence spectroscopy for melanin assessment. In a typical in vitro assay to assess melanin production in response to an external stimulus, absorption spectroscopy clearly overvalues melanin content. This method is also incapable of distinguishing non-melanotic/amelanotic control cells from those that are actually capable of performing melanogenesis. Therefore, fluorescence spectroscopy is the best method for melanin quantification as it proved to be highly specific and accurate, detecting even small variations in the synthesis of melanin. This method can also be applied to the quantification of melanin in more complex biological matrices like zebrafish embryos and human hair.
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spelling Fluorescent quantification of melaninMelaninPigment quantificationMelanin oxidationMelanin fluorescenceAbsorption spectroscopyScience & TechnologyMelanin quantification is reportedly performed by absorption spectroscopy, commonly at 405 nm. Here, we propose the implementation of fluorescence spectroscopy for melanin assessment. In a typical in vitro assay to assess melanin production in response to an external stimulus, absorption spectroscopy clearly overvalues melanin content. This method is also incapable of distinguishing non-melanotic/amelanotic control cells from those that are actually capable of performing melanogenesis. Therefore, fluorescence spectroscopy is the best method for melanin quantification as it proved to be highly specific and accurate, detecting even small variations in the synthesis of melanin. This method can also be applied to the quantification of melanin in more complex biological matrices like zebrafish embryos and human hair.This work was supported by the Portuguese Foundation for Science and Technology (FCT) through the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145- FEDER-006684) and BioTecNorte Operation (NORTE-01-0145- FEDER-000004) funded by European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. The authors would like to thank to the hair donor volunteers and to Marisa Passos, Ana Sofia Teixeira, and In^es Pinto for technical assistance regarding the collection of zebrafish embryos. The author Teresa Matam a would also like to acknowledge her postdoctoral fellowship funded by FCT (SFRH/BPD/ 102153/2014).John Wiley and SonsUniversidade do MinhoFernandes, Bruno PachecoMatamá, Maria TeresaGuimarães, Diana Isabel PereiraGomes, Andreia CCavaco-Paulo, Artur20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/43069engFernandes, Bruno P.; Matamá, Maria Teresa; Guimarães, D.; Gomes, Andreia; Cavaco-Paulo, Artur, Fluorescent quantification of melanin. Pigment Cell and Melanoma Research, 29(6), 707-712, 20161755-14711755-148X10.1111/pcmr.1253527770559http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1755-148Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-07T01:20:49Zoai:repositorium.sdum.uminho.pt:1822/43069Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:06:51.003113Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Fluorescent quantification of melanin
title Fluorescent quantification of melanin
spellingShingle Fluorescent quantification of melanin
Fernandes, Bruno Pacheco
Melanin
Pigment quantification
Melanin oxidation
Melanin fluorescence
Absorption spectroscopy
Science & Technology
title_short Fluorescent quantification of melanin
title_full Fluorescent quantification of melanin
title_fullStr Fluorescent quantification of melanin
title_full_unstemmed Fluorescent quantification of melanin
title_sort Fluorescent quantification of melanin
author Fernandes, Bruno Pacheco
author_facet Fernandes, Bruno Pacheco
Matamá, Maria Teresa
Guimarães, Diana Isabel Pereira
Gomes, Andreia C
Cavaco-Paulo, Artur
author_role author
author2 Matamá, Maria Teresa
Guimarães, Diana Isabel Pereira
Gomes, Andreia C
Cavaco-Paulo, Artur
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Fernandes, Bruno Pacheco
Matamá, Maria Teresa
Guimarães, Diana Isabel Pereira
Gomes, Andreia C
Cavaco-Paulo, Artur
dc.subject.por.fl_str_mv Melanin
Pigment quantification
Melanin oxidation
Melanin fluorescence
Absorption spectroscopy
Science & Technology
topic Melanin
Pigment quantification
Melanin oxidation
Melanin fluorescence
Absorption spectroscopy
Science & Technology
description Melanin quantification is reportedly performed by absorption spectroscopy, commonly at 405 nm. Here, we propose the implementation of fluorescence spectroscopy for melanin assessment. In a typical in vitro assay to assess melanin production in response to an external stimulus, absorption spectroscopy clearly overvalues melanin content. This method is also incapable of distinguishing non-melanotic/amelanotic control cells from those that are actually capable of performing melanogenesis. Therefore, fluorescence spectroscopy is the best method for melanin quantification as it proved to be highly specific and accurate, detecting even small variations in the synthesis of melanin. This method can also be applied to the quantification of melanin in more complex biological matrices like zebrafish embryos and human hair.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/43069
url https://hdl.handle.net/1822/43069
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fernandes, Bruno P.; Matamá, Maria Teresa; Guimarães, D.; Gomes, Andreia; Cavaco-Paulo, Artur, Fluorescent quantification of melanin. Pigment Cell and Melanoma Research, 29(6), 707-712, 2016
1755-1471
1755-148X
10.1111/pcmr.12535
27770559
http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1755-148X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley and Sons
publisher.none.fl_str_mv John Wiley and Sons
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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