Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Clarisse Marta Oliveira
Data de Publicação: 2015
Outros Autores: Pärssinen, J., Sencadas, Vítor João Gomes Silva, Correia, Vítor Manuel Gomes, Miettinen, S., Hytönen, V. P., Lanceros-Méndez, S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/38869
Resumo: This work reports on the influence of the substrate polarization of electroactive β-PVDF on human adipose stem cells (hASCs) differentiation under static and dynamic conditions. hASCs were cultured on different β-PVDF surfaces (non-poled and “poled -”) adsorbed with fibronectin and osteogenic differentiation was determined using a quantitative alkaline phosphatase assay. “Poled -” β-PVDF samples promote higher osteogenic differentiation, which is even higher under dynamic conditions. It is thus demonstrated that electroactive membranes can provide the necessary electromechanical stimuli for the differentiation of specific cells and therefore will support the design of suitable tissue engineering strategies, such as bone tissue engineering.
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spelling Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cellsElectroactive polymerStem cellOsteogenesisDynamic conditiondynamic conditionsCiências Naturais::Ciências FísicasScience & TechnologyThis work reports on the influence of the substrate polarization of electroactive β-PVDF on human adipose stem cells (hASCs) differentiation under static and dynamic conditions. hASCs were cultured on different β-PVDF surfaces (non-poled and “poled -”) adsorbed with fibronectin and osteogenic differentiation was determined using a quantitative alkaline phosphatase assay. “Poled -” β-PVDF samples promote higher osteogenic differentiation, which is even higher under dynamic conditions. It is thus demonstrated that electroactive membranes can provide the necessary electromechanical stimuli for the differentiation of specific cells and therefore will support the design of suitable tissue engineering strategies, such as bone tissue engineering.This work is funded by FEDER funds through the "Programa Operacional Fatores de Competitividade – COMPETE" and by national funds arranged by FCT- Fundação para a Ciência e a Tecnologia, project references PTDC/CTM-NAN/112574/2009 and PEST-C/FIS/UI607/2014. The authors also thank funding from Matepro –Optimizing Materials and Processes”, ref. NORTE-07-0124-FEDER-000037”, co-funded by the “Programa Operacional Regional do Norte” (ON.2 – O Novo Norte), under the “Quadro de Referência Estratégico Nacional” (QREN), through the “Fundo Europeu de Desenvolvimento Regional” (FEDER). CR, VS and VC thank the FCT for the SFRH/BPD/90870/2012, SFRH/BPD/64958/2009 and SFRH/BPD/97739/2013 grants, respectively. Academy of Finland is acknowledged for research funding (projects 136288 (VH) and 256931 (JP)).WileyUniversidade do MinhoRibeiro, Clarisse Marta OliveiraPärssinen, J.Sencadas, Vítor João Gomes SilvaCorreia, Vítor Manuel GomesMiettinen, S.Hytönen, V. P.Lanceros-Méndez, S.20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/38869eng1549-329610.1002/jbm.a.3536825370596info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:51:17Zoai:repositorium.sdum.uminho.pt:1822/38869Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:50:08.995212Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells
title Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells
spellingShingle Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells
Ribeiro, Clarisse Marta Oliveira
Electroactive polymer
Stem cell
Osteogenesis
Dynamic condition
dynamic conditions
Ciências Naturais::Ciências Físicas
Science & Technology
title_short Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells
title_full Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells
title_fullStr Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells
title_full_unstemmed Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells
title_sort Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells
author Ribeiro, Clarisse Marta Oliveira
author_facet Ribeiro, Clarisse Marta Oliveira
Pärssinen, J.
Sencadas, Vítor João Gomes Silva
Correia, Vítor Manuel Gomes
Miettinen, S.
Hytönen, V. P.
Lanceros-Méndez, S.
author_role author
author2 Pärssinen, J.
Sencadas, Vítor João Gomes Silva
Correia, Vítor Manuel Gomes
Miettinen, S.
Hytönen, V. P.
Lanceros-Méndez, S.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Ribeiro, Clarisse Marta Oliveira
Pärssinen, J.
Sencadas, Vítor João Gomes Silva
Correia, Vítor Manuel Gomes
Miettinen, S.
Hytönen, V. P.
Lanceros-Méndez, S.
dc.subject.por.fl_str_mv Electroactive polymer
Stem cell
Osteogenesis
Dynamic condition
dynamic conditions
Ciências Naturais::Ciências Físicas
Science & Technology
topic Electroactive polymer
Stem cell
Osteogenesis
Dynamic condition
dynamic conditions
Ciências Naturais::Ciências Físicas
Science & Technology
description This work reports on the influence of the substrate polarization of electroactive β-PVDF on human adipose stem cells (hASCs) differentiation under static and dynamic conditions. hASCs were cultured on different β-PVDF surfaces (non-poled and “poled -”) adsorbed with fibronectin and osteogenic differentiation was determined using a quantitative alkaline phosphatase assay. “Poled -” β-PVDF samples promote higher osteogenic differentiation, which is even higher under dynamic conditions. It is thus demonstrated that electroactive membranes can provide the necessary electromechanical stimuli for the differentiation of specific cells and therefore will support the design of suitable tissue engineering strategies, such as bone tissue engineering.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/38869
url https://hdl.handle.net/1822/38869
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1549-3296
10.1002/jbm.a.35368
25370596
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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