Enhancement of adhesion and promotion of osteogenic differentiation of human adipose stem cells by poled electroactive poly(vinylidene fluoride)

Detalhes bibliográficos
Autor(a) principal: Parssinen, J.
Data de Publicação: 2015
Outros Autores: Hammarén, H., Rahikainen, R., Sencadas, Vítor João Gomes Silva, Ribeiro, C., Vanhatupa, S., Miettinen, S., Lanceros-Méndez, S., Hytönen, V. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/38879
Resumo: Poly(vinylidene fluoride) (PVDF) is a biocompatible material with excellent electroactive properties. Non-electroactive α-PVDF and electroactive β-PVDF were used to investigate the substrate polarization and polarity influence on the focal adhesion size and number as well as on human adipose stem cells (hASCs) differentiation. hASCs were cultured on different PVDF surfaces adsorbed with fibronectin and focal adhesion size and number, total adhesion area, cell size, cell aspect ratio and focal adhesion density were estimated using cells expressing EGFP-vinculin. Osteogenic differentiation was also determined using a quantitative alkaline phosphatase assay. The surface charge of the poled PVDF films (positive or negative) influenced the hydrophobicity of the samples, leading to variations in the conformation of adsorbed extracellular matrix (ECM) proteins, which ultimately modulated the stem cell adhesion on the films and induced their osteogenic differentiation.
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spelling Enhancement of adhesion and promotion of osteogenic differentiation of human adipose stem cells by poled electroactive poly(vinylidene fluoride)Electroactive polymerStem cellCell adhesionOsteogenesiosteogenesisCiências Naturais::Ciências FísicasScience & TechnologyPoly(vinylidene fluoride) (PVDF) is a biocompatible material with excellent electroactive properties. Non-electroactive α-PVDF and electroactive β-PVDF were used to investigate the substrate polarization and polarity influence on the focal adhesion size and number as well as on human adipose stem cells (hASCs) differentiation. hASCs were cultured on different PVDF surfaces adsorbed with fibronectin and focal adhesion size and number, total adhesion area, cell size, cell aspect ratio and focal adhesion density were estimated using cells expressing EGFP-vinculin. Osteogenic differentiation was also determined using a quantitative alkaline phosphatase assay. The surface charge of the poled PVDF films (positive or negative) influenced the hydrophobicity of the samples, leading to variations in the conformation of adsorbed extracellular matrix (ECM) proteins, which ultimately modulated the stem cell adhesion on the films and induced their osteogenic differentiation.The study was supported financially by the Academy of Finland (136288, 140978 and 256931), the Sigrid Jusélius Foundation, the Pirkanmaa Hospital District and the Finnish Funding Agency for Technology and Innovation (TEKES). This study was also supported by FEDER through the COMPETE Program, by the Portuguese Foundation for Science and Technology (FCT) in the framework of the Strategic Project PEST- C/FIS/UI607/2011 and by projects NANO/NMed-SD/0156/2007 and PTDC/CTM NAN/112574/2009. The autors also thank the project Matepro – Optimizing Materials and Processes”, ref. NORTE-07-0124-FEDER-000037”, co-funded by the “Programa Operacional Regional do Norte” (ON.2 – O Novo Norte), under the “Quadro de Referência Estratégico Nacional” (QREN), through the “Fundo Europeu de Desenvolvimento Regional” (FEDER). V.S. and C.R. thank the FCT for the SFRH/BPD/63148/2009 and SFRH/BPD/90870/2012 grants, respectively.WileyUniversidade do MinhoParssinen, J.Hammarén, H.Rahikainen, R.Sencadas, Vítor João Gomes SilvaRibeiro, C.Vanhatupa, S.Miettinen, S.Lanceros-Méndez, S.Hytönen, V. P.20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/38879engParssinen, J., Hammaren, H., Rahikainen, R., Sencadas, V., Ribeiro, C., Vanhatupa, S., . . . Hytonen, V. P. (2015). Enhancement of adhesion and promotion of osteogenic differentiation of human adipose stem cells by poled electroactive poly(vinylidene fluoride). Journal of Biomedical Materials Research Part A, 103(3), 919-928. doi: 10.1002/jbm.a.352341549-329610.1002/jbm.a.3523424838756info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:11:11Zoai:repositorium.sdum.uminho.pt:1822/38879Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:02:54.439838Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Enhancement of adhesion and promotion of osteogenic differentiation of human adipose stem cells by poled electroactive poly(vinylidene fluoride)
title Enhancement of adhesion and promotion of osteogenic differentiation of human adipose stem cells by poled electroactive poly(vinylidene fluoride)
spellingShingle Enhancement of adhesion and promotion of osteogenic differentiation of human adipose stem cells by poled electroactive poly(vinylidene fluoride)
Parssinen, J.
Electroactive polymer
Stem cell
Cell adhesion
Osteogenesi
osteogenesis
Ciências Naturais::Ciências Físicas
Science & Technology
title_short Enhancement of adhesion and promotion of osteogenic differentiation of human adipose stem cells by poled electroactive poly(vinylidene fluoride)
title_full Enhancement of adhesion and promotion of osteogenic differentiation of human adipose stem cells by poled electroactive poly(vinylidene fluoride)
title_fullStr Enhancement of adhesion and promotion of osteogenic differentiation of human adipose stem cells by poled electroactive poly(vinylidene fluoride)
title_full_unstemmed Enhancement of adhesion and promotion of osteogenic differentiation of human adipose stem cells by poled electroactive poly(vinylidene fluoride)
title_sort Enhancement of adhesion and promotion of osteogenic differentiation of human adipose stem cells by poled electroactive poly(vinylidene fluoride)
author Parssinen, J.
author_facet Parssinen, J.
Hammarén, H.
Rahikainen, R.
Sencadas, Vítor João Gomes Silva
Ribeiro, C.
Vanhatupa, S.
Miettinen, S.
Lanceros-Méndez, S.
Hytönen, V. P.
author_role author
author2 Hammarén, H.
Rahikainen, R.
Sencadas, Vítor João Gomes Silva
Ribeiro, C.
Vanhatupa, S.
Miettinen, S.
Lanceros-Méndez, S.
Hytönen, V. P.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Parssinen, J.
Hammarén, H.
Rahikainen, R.
Sencadas, Vítor João Gomes Silva
Ribeiro, C.
Vanhatupa, S.
Miettinen, S.
Lanceros-Méndez, S.
Hytönen, V. P.
dc.subject.por.fl_str_mv Electroactive polymer
Stem cell
Cell adhesion
Osteogenesi
osteogenesis
Ciências Naturais::Ciências Físicas
Science & Technology
topic Electroactive polymer
Stem cell
Cell adhesion
Osteogenesi
osteogenesis
Ciências Naturais::Ciências Físicas
Science & Technology
description Poly(vinylidene fluoride) (PVDF) is a biocompatible material with excellent electroactive properties. Non-electroactive α-PVDF and electroactive β-PVDF were used to investigate the substrate polarization and polarity influence on the focal adhesion size and number as well as on human adipose stem cells (hASCs) differentiation. hASCs were cultured on different PVDF surfaces adsorbed with fibronectin and focal adhesion size and number, total adhesion area, cell size, cell aspect ratio and focal adhesion density were estimated using cells expressing EGFP-vinculin. Osteogenic differentiation was also determined using a quantitative alkaline phosphatase assay. The surface charge of the poled PVDF films (positive or negative) influenced the hydrophobicity of the samples, leading to variations in the conformation of adsorbed extracellular matrix (ECM) proteins, which ultimately modulated the stem cell adhesion on the films and induced their osteogenic differentiation.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/38879
url https://hdl.handle.net/1822/38879
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Parssinen, J., Hammaren, H., Rahikainen, R., Sencadas, V., Ribeiro, C., Vanhatupa, S., . . . Hytonen, V. P. (2015). Enhancement of adhesion and promotion of osteogenic differentiation of human adipose stem cells by poled electroactive poly(vinylidene fluoride). Journal of Biomedical Materials Research Part A, 103(3), 919-928. doi: 10.1002/jbm.a.35234
1549-3296
10.1002/jbm.a.35234
24838756
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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