Electrochemical determination of antioxidant capacities in flavored waters by guanine and adenine biosensors

Detalhes bibliográficos
Autor(a) principal: Kamel, Ayman H.
Data de Publicação: 2008
Outros Autores: Moreira, Felismina T. C., Delerue-Matos, Cristina, Sales, M. Goreti F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/2939
Resumo: The immobilization and electro-oxidation of guanine and adenine asDNA bases on glassy carbon electrode are evaluated by square wave voltammetric analysis. The influence of electrochemical pretreatments, nature of supporting electrolyte, pH, accumulation time and composition of DNA nucleotides on the immobilization effect and the electrochemical mechanism are discussed. Trace levels of either guanine or adenine can be readily detected following short accumulation time with detection limits of 35 and 40 ngmL−1 for guanine and adenine, respectively. The biosensors of guanine and adenine were employed for the voltammetric detection of antioxidant capacity in flavored water samples. The method relies on monitoring the changes of the intrinsic anodic response of the surface-confined guanine and adenine species, resulting from its interaction with free radicals from Fenton-type reaction in absence and presence of antioxidant. Ascorbic acid was used as standard to evaluate antioxidant capacities of samples. Analytical data was compared with that of FRAP method.
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spelling Electrochemical determination of antioxidant capacities in flavored waters by guanine and adenine biosensorsGuanineAdenineVoltammetryFlavored watersAntioxidant capacityThe immobilization and electro-oxidation of guanine and adenine asDNA bases on glassy carbon electrode are evaluated by square wave voltammetric analysis. The influence of electrochemical pretreatments, nature of supporting electrolyte, pH, accumulation time and composition of DNA nucleotides on the immobilization effect and the electrochemical mechanism are discussed. Trace levels of either guanine or adenine can be readily detected following short accumulation time with detection limits of 35 and 40 ngmL−1 for guanine and adenine, respectively. The biosensors of guanine and adenine were employed for the voltammetric detection of antioxidant capacity in flavored water samples. The method relies on monitoring the changes of the intrinsic anodic response of the surface-confined guanine and adenine species, resulting from its interaction with free radicals from Fenton-type reaction in absence and presence of antioxidant. Ascorbic acid was used as standard to evaluate antioxidant capacities of samples. Analytical data was compared with that of FRAP method.ElsevierRepositório Científico do Instituto Politécnico do PortoKamel, Ayman H.Moreira, Felismina T. C.Delerue-Matos, CristinaSales, M. Goreti F.2013-11-25T12:56:14Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/2939eng0956-566310.1016/j.bios.2008.06.007info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:42:33Zoai:recipp.ipp.pt:10400.22/2939Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:23:58.085652Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Electrochemical determination of antioxidant capacities in flavored waters by guanine and adenine biosensors
title Electrochemical determination of antioxidant capacities in flavored waters by guanine and adenine biosensors
spellingShingle Electrochemical determination of antioxidant capacities in flavored waters by guanine and adenine biosensors
Kamel, Ayman H.
Guanine
Adenine
Voltammetry
Flavored waters
Antioxidant capacity
title_short Electrochemical determination of antioxidant capacities in flavored waters by guanine and adenine biosensors
title_full Electrochemical determination of antioxidant capacities in flavored waters by guanine and adenine biosensors
title_fullStr Electrochemical determination of antioxidant capacities in flavored waters by guanine and adenine biosensors
title_full_unstemmed Electrochemical determination of antioxidant capacities in flavored waters by guanine and adenine biosensors
title_sort Electrochemical determination of antioxidant capacities in flavored waters by guanine and adenine biosensors
author Kamel, Ayman H.
author_facet Kamel, Ayman H.
Moreira, Felismina T. C.
Delerue-Matos, Cristina
Sales, M. Goreti F.
author_role author
author2 Moreira, Felismina T. C.
Delerue-Matos, Cristina
Sales, M. Goreti F.
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Kamel, Ayman H.
Moreira, Felismina T. C.
Delerue-Matos, Cristina
Sales, M. Goreti F.
dc.subject.por.fl_str_mv Guanine
Adenine
Voltammetry
Flavored waters
Antioxidant capacity
topic Guanine
Adenine
Voltammetry
Flavored waters
Antioxidant capacity
description The immobilization and electro-oxidation of guanine and adenine asDNA bases on glassy carbon electrode are evaluated by square wave voltammetric analysis. The influence of electrochemical pretreatments, nature of supporting electrolyte, pH, accumulation time and composition of DNA nucleotides on the immobilization effect and the electrochemical mechanism are discussed. Trace levels of either guanine or adenine can be readily detected following short accumulation time with detection limits of 35 and 40 ngmL−1 for guanine and adenine, respectively. The biosensors of guanine and adenine were employed for the voltammetric detection of antioxidant capacity in flavored water samples. The method relies on monitoring the changes of the intrinsic anodic response of the surface-confined guanine and adenine species, resulting from its interaction with free radicals from Fenton-type reaction in absence and presence of antioxidant. Ascorbic acid was used as standard to evaluate antioxidant capacities of samples. Analytical data was compared with that of FRAP method.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
2013-11-25T12:56:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/2939
url http://hdl.handle.net/10400.22/2939
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0956-5663
10.1016/j.bios.2008.06.007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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