Voltammetric determination of all DNA nucleotides

Detalhes bibliográficos
Autor(a) principal: Brett, A. M. Oliveira
Data de Publicação: 2004
Outros Autores: Piedade, J. A. P., Silva, L. A., Diculescu, V. C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/5126
https://doi.org/10.1016/j.ab.2004.06.021
Resumo: The voltammetric oxidation of all deoxyribonucleic acid (DNA) monophosphate nucleotides is investigated for the first time over a wide pH range by differential pulse voltammetry with a glassy carbon electrode. Experimental conditions such as the electrode size, supporting electrolyte composition, and pH were optimized to obtain the best peak potential separation and higher currents. This enabled the simultaneous voltammetric determination of all four DNA bases in equimolar mixtures and detection limits in the nanomolar range at physiological pH. It was also possible to detect for the first time the oxidation of each of the purine and pyrimidine nucleotides free in solution or as monomers in single-stranded DNA.
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spelling Voltammetric determination of all DNA nucleotidesGuanineAdenineThymineCytosineDeoxynucleosidesDeoxynucleotidesVoltammetryAnodic oxidationThe voltammetric oxidation of all deoxyribonucleic acid (DNA) monophosphate nucleotides is investigated for the first time over a wide pH range by differential pulse voltammetry with a glassy carbon electrode. Experimental conditions such as the electrode size, supporting electrolyte composition, and pH were optimized to obtain the best peak potential separation and higher currents. This enabled the simultaneous voltammetric determination of all four DNA bases in equimolar mixtures and detection limits in the nanomolar range at physiological pH. It was also possible to detect for the first time the oxidation of each of the purine and pyrimidine nucleotides free in solution or as monomers in single-stranded DNA.http://www.sciencedirect.com/science/article/B6W9V-4CX6X18-5/1/3ffa036b25c6e90ed00b379e1f79c7b92004info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5126http://hdl.handle.net/10316/5126https://doi.org/10.1016/j.ab.2004.06.021engAnalytical Biochemistry. 332:2 (2004) 321-329Brett, A. M. OliveiraPiedade, J. A. P.Silva, L. A.Diculescu, V. C.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-11-06T16:48:43Zoai:estudogeral.uc.pt:10316/5126Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:14.542903Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Voltammetric determination of all DNA nucleotides
title Voltammetric determination of all DNA nucleotides
spellingShingle Voltammetric determination of all DNA nucleotides
Brett, A. M. Oliveira
Guanine
Adenine
Thymine
Cytosine
Deoxynucleosides
Deoxynucleotides
Voltammetry
Anodic oxidation
title_short Voltammetric determination of all DNA nucleotides
title_full Voltammetric determination of all DNA nucleotides
title_fullStr Voltammetric determination of all DNA nucleotides
title_full_unstemmed Voltammetric determination of all DNA nucleotides
title_sort Voltammetric determination of all DNA nucleotides
author Brett, A. M. Oliveira
author_facet Brett, A. M. Oliveira
Piedade, J. A. P.
Silva, L. A.
Diculescu, V. C.
author_role author
author2 Piedade, J. A. P.
Silva, L. A.
Diculescu, V. C.
author2_role author
author
author
dc.contributor.author.fl_str_mv Brett, A. M. Oliveira
Piedade, J. A. P.
Silva, L. A.
Diculescu, V. C.
dc.subject.por.fl_str_mv Guanine
Adenine
Thymine
Cytosine
Deoxynucleosides
Deoxynucleotides
Voltammetry
Anodic oxidation
topic Guanine
Adenine
Thymine
Cytosine
Deoxynucleosides
Deoxynucleotides
Voltammetry
Anodic oxidation
description The voltammetric oxidation of all deoxyribonucleic acid (DNA) monophosphate nucleotides is investigated for the first time over a wide pH range by differential pulse voltammetry with a glassy carbon electrode. Experimental conditions such as the electrode size, supporting electrolyte composition, and pH were optimized to obtain the best peak potential separation and higher currents. This enabled the simultaneous voltammetric determination of all four DNA bases in equimolar mixtures and detection limits in the nanomolar range at physiological pH. It was also possible to detect for the first time the oxidation of each of the purine and pyrimidine nucleotides free in solution or as monomers in single-stranded DNA.
publishDate 2004
dc.date.none.fl_str_mv 2004
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/5126
http://hdl.handle.net/10316/5126
https://doi.org/10.1016/j.ab.2004.06.021
url http://hdl.handle.net/10316/5126
https://doi.org/10.1016/j.ab.2004.06.021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Analytical Biochemistry. 332:2 (2004) 321-329
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv aplication/PDF
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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