Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/21866 |
Resumo: | This paper presents a potentiometric biosensor for the detection of amyloidβ-42 (Aβ-42) inpoint-of-care analysis. This approach is based on the molecular imprint polymer (MIP) technique,which uses covalently immobilised Aβ-42 to create specific detection cavities on the surface of single-walled carbon nanotubes (SWCNTs). The biosensor was prepared by binding Aβ-42 to the SWCNTsurface and then imprinting it by adding acrylamide (monomer), N,N′-methylene-bis-acrylamide(crosslinker) and ammonium persulphate (initiator). The target peptide was removed from thepolymer matrix by the proteolytic action of an enzyme (proteinase K). The presence of imprintingsites was confirmed by comparing a MIP-modified surface with a negative control (NIP) consistingof a similar material where the target molecule had been removed from the process. The abilityof the sensing material to rebind Aβ-42 was demonstrated by incorporating the MIP material asan electroactive compound in a PVC/plasticiser mixture applied to a solid conductive support ofgraphite. All steps of the synthesis of the imprinted materials were followed by Raman spectroscopyand Fourier transform infrared spectroscopy (FTIR). The analytical performance was evaluated bypotentiometric transduction, and the MIP material showed cationic slopes of 75 mV-decade−1inbuffer pH 8.0 and a detection limit of 0.72μg/mL. Overall, potentiometric transduction confirmedthat the sensor can discriminate Aβ-42 in the presence of other biomolecules in the same solution |
id |
RCAP_59948476a03b0536ba00a038c2ce6af0 |
---|---|
oai_identifier_str |
oai:recipp.ipp.pt:10400.22/21866 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker DetectionAmyloidβ-42Molecularly imprinted polymersPotentiometric sensorAlzheimer biomarkerThis paper presents a potentiometric biosensor for the detection of amyloidβ-42 (Aβ-42) inpoint-of-care analysis. This approach is based on the molecular imprint polymer (MIP) technique,which uses covalently immobilised Aβ-42 to create specific detection cavities on the surface of single-walled carbon nanotubes (SWCNTs). The biosensor was prepared by binding Aβ-42 to the SWCNTsurface and then imprinting it by adding acrylamide (monomer), N,N′-methylene-bis-acrylamide(crosslinker) and ammonium persulphate (initiator). The target peptide was removed from thepolymer matrix by the proteolytic action of an enzyme (proteinase K). The presence of imprintingsites was confirmed by comparing a MIP-modified surface with a negative control (NIP) consistingof a similar material where the target molecule had been removed from the process. The abilityof the sensing material to rebind Aβ-42 was demonstrated by incorporating the MIP material asan electroactive compound in a PVC/plasticiser mixture applied to a solid conductive support ofgraphite. All steps of the synthesis of the imprinted materials were followed by Raman spectroscopyand Fourier transform infrared spectroscopy (FTIR). The analytical performance was evaluated bypotentiometric transduction, and the MIP material showed cationic slopes of 75 mV-decade−1inbuffer pH 8.0 and a detection limit of 0.72μg/mL. Overall, potentiometric transduction confirmedthat the sensor can discriminate Aβ-42 in the presence of other biomolecules in the same solution0624_2IQBIONEURO_6_E, 2QBioneuro, Impulso de una red de I + i en química biológica para diagnóstico y tratamiento de enfermedades neurológicas EP-INTERREG V A España Portugal (POCTEP).MDPIRepositório Científico do Instituto Politécnico do PortoRibeiro, Sónia ClaúdiaFernandes, RúbenMoreira, FelisminaFerreira Sales, Maria Goreti2023-01-25T16:17:52Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/21866eng10.3390/app12073625info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:18:12Zoai:recipp.ipp.pt:10400.22/21866Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:41:57.124478Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection |
title |
Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection |
spellingShingle |
Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection Ribeiro, Sónia Claúdia Amyloidβ-42 Molecularly imprinted polymers Potentiometric sensor Alzheimer biomarker |
title_short |
Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection |
title_full |
Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection |
title_fullStr |
Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection |
title_full_unstemmed |
Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection |
title_sort |
Potentiometric Biosensor Based on Artificial Antibodies for an Alzheimer Biomarker Detection |
author |
Ribeiro, Sónia Claúdia |
author_facet |
Ribeiro, Sónia Claúdia Fernandes, Rúben Moreira, Felismina Ferreira Sales, Maria Goreti |
author_role |
author |
author2 |
Fernandes, Rúben Moreira, Felismina Ferreira Sales, Maria Goreti |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Ribeiro, Sónia Claúdia Fernandes, Rúben Moreira, Felismina Ferreira Sales, Maria Goreti |
dc.subject.por.fl_str_mv |
Amyloidβ-42 Molecularly imprinted polymers Potentiometric sensor Alzheimer biomarker |
topic |
Amyloidβ-42 Molecularly imprinted polymers Potentiometric sensor Alzheimer biomarker |
description |
This paper presents a potentiometric biosensor for the detection of amyloidβ-42 (Aβ-42) inpoint-of-care analysis. This approach is based on the molecular imprint polymer (MIP) technique,which uses covalently immobilised Aβ-42 to create specific detection cavities on the surface of single-walled carbon nanotubes (SWCNTs). The biosensor was prepared by binding Aβ-42 to the SWCNTsurface and then imprinting it by adding acrylamide (monomer), N,N′-methylene-bis-acrylamide(crosslinker) and ammonium persulphate (initiator). The target peptide was removed from thepolymer matrix by the proteolytic action of an enzyme (proteinase K). The presence of imprintingsites was confirmed by comparing a MIP-modified surface with a negative control (NIP) consistingof a similar material where the target molecule had been removed from the process. The abilityof the sensing material to rebind Aβ-42 was demonstrated by incorporating the MIP material asan electroactive compound in a PVC/plasticiser mixture applied to a solid conductive support ofgraphite. All steps of the synthesis of the imprinted materials were followed by Raman spectroscopyand Fourier transform infrared spectroscopy (FTIR). The analytical performance was evaluated bypotentiometric transduction, and the MIP material showed cationic slopes of 75 mV-decade−1inbuffer pH 8.0 and a detection limit of 0.72μg/mL. Overall, potentiometric transduction confirmedthat the sensor can discriminate Aβ-42 in the presence of other biomolecules in the same solution |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 2022-01-01T00:00:00Z 2023-01-25T16:17:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/21866 |
url |
http://hdl.handle.net/10400.22/21866 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.3390/app12073625 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799131506180882432 |