Prolonged intracellular accumulation of light-inducible nanoparticles in leukemia cells allows their remote activation
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/108282 https://doi.org/10.1038/ncomms15204 |
Resumo: | Leukaemia cells that are resistant to conventional therapies are thought to reside in protective niches. Here, we describe light-inducible polymeric retinoic acid (RA)-containing nanoparticles (NPs) with the capacity to accumulate in the cytoplasm of leukaemia cells for several days and release their RA payloads within a few minutes upon exposure to blue/UV light. Compared to NPs that are not activated by light exposure, these NPs more efficiently reduce the clonogenicity of bone marrow cancer cells from patients with acute myeloid leukaemia (AML) and induce the differentiation of RA-low sensitive leukaemia cells. Importantly, we show that leukaemia cells transfected with light-inducible NPs containing RA can engraft into bone marrow in vivo in the proximity of other leukaemic cells, differentiate upon exposure to blue light and release paracrine factors that modulate nearby cells. The NPs described here offer a promising strategy for controlling distant cell populations and remotely modulating leukaemic niches. |
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Prolonged intracellular accumulation of light-inducible nanoparticles in leukemia cells allows their remote activationAgedAged, 80 and overAnimalsBenzene DerivativesCell Line, TumorDrug CompoundingFemaleFormatesHuman Umbilical Vein Endothelial CellsHumansLeukemia, Promyelocytic, AcuteLeukocytesLightMaleMiceMice, Inbred NODNanoparticlesPhotosensitizing AgentsPolyethyleneimineTretinoinU937 CellsXenograft Model Antitumor AssaysLeukaemia cells that are resistant to conventional therapies are thought to reside in protective niches. Here, we describe light-inducible polymeric retinoic acid (RA)-containing nanoparticles (NPs) with the capacity to accumulate in the cytoplasm of leukaemia cells for several days and release their RA payloads within a few minutes upon exposure to blue/UV light. Compared to NPs that are not activated by light exposure, these NPs more efficiently reduce the clonogenicity of bone marrow cancer cells from patients with acute myeloid leukaemia (AML) and induce the differentiation of RA-low sensitive leukaemia cells. Importantly, we show that leukaemia cells transfected with light-inducible NPs containing RA can engraft into bone marrow in vivo in the proximity of other leukaemic cells, differentiate upon exposure to blue light and release paracrine factors that modulate nearby cells. The NPs described here offer a promising strategy for controlling distant cell populations and remotely modulating leukaemic niches.Springer Nature2017-05-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/108282http://hdl.handle.net/10316/108282https://doi.org/10.1038/ncomms15204eng2041-1723Boto, CarlosQuartin, EmanuelCai, YijunMartín-Lorenzo, AlbertoCenador, María Begoña GarcíaPinto, SandraGupta, RajeevEnver, TariqSánchez-García, IsidroHong, DengliNeves, Ricardo Pires dasFerreira, Linoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-22T11:08:51Zoai:estudogeral.uc.pt:10316/108282Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:24:35.363778Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Prolonged intracellular accumulation of light-inducible nanoparticles in leukemia cells allows their remote activation |
title |
Prolonged intracellular accumulation of light-inducible nanoparticles in leukemia cells allows their remote activation |
spellingShingle |
Prolonged intracellular accumulation of light-inducible nanoparticles in leukemia cells allows their remote activation Boto, Carlos Aged Aged, 80 and over Animals Benzene Derivatives Cell Line, Tumor Drug Compounding Female Formates Human Umbilical Vein Endothelial Cells Humans Leukemia, Promyelocytic, Acute Leukocytes Light Male Mice Mice, Inbred NOD Nanoparticles Photosensitizing Agents Polyethyleneimine Tretinoin U937 Cells Xenograft Model Antitumor Assays |
title_short |
Prolonged intracellular accumulation of light-inducible nanoparticles in leukemia cells allows their remote activation |
title_full |
Prolonged intracellular accumulation of light-inducible nanoparticles in leukemia cells allows their remote activation |
title_fullStr |
Prolonged intracellular accumulation of light-inducible nanoparticles in leukemia cells allows their remote activation |
title_full_unstemmed |
Prolonged intracellular accumulation of light-inducible nanoparticles in leukemia cells allows their remote activation |
title_sort |
Prolonged intracellular accumulation of light-inducible nanoparticles in leukemia cells allows their remote activation |
author |
Boto, Carlos |
author_facet |
Boto, Carlos Quartin, Emanuel Cai, Yijun Martín-Lorenzo, Alberto Cenador, María Begoña García Pinto, Sandra Gupta, Rajeev Enver, Tariq Sánchez-García, Isidro Hong, Dengli Neves, Ricardo Pires das Ferreira, Lino |
author_role |
author |
author2 |
Quartin, Emanuel Cai, Yijun Martín-Lorenzo, Alberto Cenador, María Begoña García Pinto, Sandra Gupta, Rajeev Enver, Tariq Sánchez-García, Isidro Hong, Dengli Neves, Ricardo Pires das Ferreira, Lino |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Boto, Carlos Quartin, Emanuel Cai, Yijun Martín-Lorenzo, Alberto Cenador, María Begoña García Pinto, Sandra Gupta, Rajeev Enver, Tariq Sánchez-García, Isidro Hong, Dengli Neves, Ricardo Pires das Ferreira, Lino |
dc.subject.por.fl_str_mv |
Aged Aged, 80 and over Animals Benzene Derivatives Cell Line, Tumor Drug Compounding Female Formates Human Umbilical Vein Endothelial Cells Humans Leukemia, Promyelocytic, Acute Leukocytes Light Male Mice Mice, Inbred NOD Nanoparticles Photosensitizing Agents Polyethyleneimine Tretinoin U937 Cells Xenograft Model Antitumor Assays |
topic |
Aged Aged, 80 and over Animals Benzene Derivatives Cell Line, Tumor Drug Compounding Female Formates Human Umbilical Vein Endothelial Cells Humans Leukemia, Promyelocytic, Acute Leukocytes Light Male Mice Mice, Inbred NOD Nanoparticles Photosensitizing Agents Polyethyleneimine Tretinoin U937 Cells Xenograft Model Antitumor Assays |
description |
Leukaemia cells that are resistant to conventional therapies are thought to reside in protective niches. Here, we describe light-inducible polymeric retinoic acid (RA)-containing nanoparticles (NPs) with the capacity to accumulate in the cytoplasm of leukaemia cells for several days and release their RA payloads within a few minutes upon exposure to blue/UV light. Compared to NPs that are not activated by light exposure, these NPs more efficiently reduce the clonogenicity of bone marrow cancer cells from patients with acute myeloid leukaemia (AML) and induce the differentiation of RA-low sensitive leukaemia cells. Importantly, we show that leukaemia cells transfected with light-inducible NPs containing RA can engraft into bone marrow in vivo in the proximity of other leukaemic cells, differentiate upon exposure to blue light and release paracrine factors that modulate nearby cells. The NPs described here offer a promising strategy for controlling distant cell populations and remotely modulating leukaemic niches. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-11 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/108282 http://hdl.handle.net/10316/108282 https://doi.org/10.1038/ncomms15204 |
url |
http://hdl.handle.net/10316/108282 https://doi.org/10.1038/ncomms15204 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2041-1723 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Springer Nature |
publisher.none.fl_str_mv |
Springer Nature |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799134129931943936 |