Contactless resolution of inflammatory signals in tailored macrophage-based cell therapeutics
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/88771 |
Resumo: | In recent years, nanotechnology-based microRNA (miR) therapeutic platforms have shown great promise for immunotherapy and tissue regeneration, despite the unmet challenge of achieving efficient and safe delivery of miRs. The transport of miRs offers precision and regulatory value for a myriad of biological processes and pathways, including the control of macrophage (MÏ ) functions and, consequently, the inflammatory cascades MÏ are involved in. Thus, enforcement of MÏ can boost the regenerative process and provide new solutions for diverse chronic pathologies. In this study, we sought to develop a magnetically guided transporter to deliver an miR-155 antagonist to M1-primed MÏ . Furthermore, we determined its modulatory effect in reprogramming MÏ from inflammatory to pro-regenerative phenotypes, with the aim of tissue healing and regenerative medicine approaches. This strategy combines contactless and high-precision control of MÏ , anticipating new functional miR carriers for targeted strategies controlled by extracorporeal action. The magnetoplexes SPION@PEI-miR were efficiently delivered into MÏ without compromising cell viability and successfully induced miR-mediated gene silencing by enhancing the expression of anti-inflammatory markers (IL4 and IL10) and the production of M2Ï -related markers (CD206 and IL4). Given its multimodal features, SPION@PEI-miR represents a simple, safe, and nonviral theranostic platform that enables imaging, tracking, and miR delivery with modulatory effects on immune cells. |
id |
RCAP_59f1ed44cc58c306e93a459dee67b54a |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/88771 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Contactless resolution of inflammatory signals in tailored macrophage-based cell therapeuticsMacrophagesMagnetoplexesmiR-155RNA deliverySPIONIn recent years, nanotechnology-based microRNA (miR) therapeutic platforms have shown great promise for immunotherapy and tissue regeneration, despite the unmet challenge of achieving efficient and safe delivery of miRs. The transport of miRs offers precision and regulatory value for a myriad of biological processes and pathways, including the control of macrophage (MÏ ) functions and, consequently, the inflammatory cascades MÏ are involved in. Thus, enforcement of MÏ can boost the regenerative process and provide new solutions for diverse chronic pathologies. In this study, we sought to develop a magnetically guided transporter to deliver an miR-155 antagonist to M1-primed MÏ . Furthermore, we determined its modulatory effect in reprogramming MÏ from inflammatory to pro-regenerative phenotypes, with the aim of tissue healing and regenerative medicine approaches. This strategy combines contactless and high-precision control of MÏ , anticipating new functional miR carriers for targeted strategies controlled by extracorporeal action. The magnetoplexes SPION@PEI-miR were efficiently delivered into MÏ without compromising cell viability and successfully induced miR-mediated gene silencing by enhancing the expression of anti-inflammatory markers (IL4 and IL10) and the production of M2Ï -related markers (CD206 and IL4). Given its multimodal features, SPION@PEI-miR represents a simple, safe, and nonviral theranostic platform that enables imaging, tracking, and miR delivery with modulatory effects on immune cells.This research was funded by the European Research Council, Consolidator Grant MagTendon, grant number 772817 and by the FCT - Fundação para a Ciência e a Tecnologia, scholarship number SFRD/BD/144816/2019.American Chemical Society (ACS)Universidade do MinhoAlmeida, Ana F.Miranda, Margarida SilvaVinhas, Carla Adriana AraújoRodrigues, Márcia T.Gomes, Manuela E.2023-082023-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/88771engAlmeida A. F., Miranda M. S., Vinhas A., Rodrigues M. T., Gomes M. E. Contactless Resolution of Inflammatory Signals in Tailored Macrophage-Based Cell Therapeutics, ACS Appl Mater Interfaces , Vol. 15, Issue 44, pp. 50612–50625, doi:10.1021/acsami.2c22505., 20231944-824410.1021/acsami.2c22505.https://doi.org/10.1021/acsami.2c22505info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-17T01:17:44Zoai:repositorium.sdum.uminho.pt:1822/88771Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:38:31.446195Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Contactless resolution of inflammatory signals in tailored macrophage-based cell therapeutics |
title |
Contactless resolution of inflammatory signals in tailored macrophage-based cell therapeutics |
spellingShingle |
Contactless resolution of inflammatory signals in tailored macrophage-based cell therapeutics Almeida, Ana F. Macrophages Magnetoplexes miR-155 RNA delivery SPION |
title_short |
Contactless resolution of inflammatory signals in tailored macrophage-based cell therapeutics |
title_full |
Contactless resolution of inflammatory signals in tailored macrophage-based cell therapeutics |
title_fullStr |
Contactless resolution of inflammatory signals in tailored macrophage-based cell therapeutics |
title_full_unstemmed |
Contactless resolution of inflammatory signals in tailored macrophage-based cell therapeutics |
title_sort |
Contactless resolution of inflammatory signals in tailored macrophage-based cell therapeutics |
author |
Almeida, Ana F. |
author_facet |
Almeida, Ana F. Miranda, Margarida Silva Vinhas, Carla Adriana Araújo Rodrigues, Márcia T. Gomes, Manuela E. |
author_role |
author |
author2 |
Miranda, Margarida Silva Vinhas, Carla Adriana Araújo Rodrigues, Márcia T. Gomes, Manuela E. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Almeida, Ana F. Miranda, Margarida Silva Vinhas, Carla Adriana Araújo Rodrigues, Márcia T. Gomes, Manuela E. |
dc.subject.por.fl_str_mv |
Macrophages Magnetoplexes miR-155 RNA delivery SPION |
topic |
Macrophages Magnetoplexes miR-155 RNA delivery SPION |
description |
In recent years, nanotechnology-based microRNA (miR) therapeutic platforms have shown great promise for immunotherapy and tissue regeneration, despite the unmet challenge of achieving efficient and safe delivery of miRs. The transport of miRs offers precision and regulatory value for a myriad of biological processes and pathways, including the control of macrophage (MÏ ) functions and, consequently, the inflammatory cascades MÏ are involved in. Thus, enforcement of MÏ can boost the regenerative process and provide new solutions for diverse chronic pathologies. In this study, we sought to develop a magnetically guided transporter to deliver an miR-155 antagonist to M1-primed MÏ . Furthermore, we determined its modulatory effect in reprogramming MÏ from inflammatory to pro-regenerative phenotypes, with the aim of tissue healing and regenerative medicine approaches. This strategy combines contactless and high-precision control of MÏ , anticipating new functional miR carriers for targeted strategies controlled by extracorporeal action. The magnetoplexes SPION@PEI-miR were efficiently delivered into MÏ without compromising cell viability and successfully induced miR-mediated gene silencing by enhancing the expression of anti-inflammatory markers (IL4 and IL10) and the production of M2Ï -related markers (CD206 and IL4). Given its multimodal features, SPION@PEI-miR represents a simple, safe, and nonviral theranostic platform that enables imaging, tracking, and miR delivery with modulatory effects on immune cells. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-08 2023-08-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/88771 |
url |
https://hdl.handle.net/1822/88771 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Almeida A. F., Miranda M. S., Vinhas A., Rodrigues M. T., Gomes M. E. Contactless Resolution of Inflammatory Signals in Tailored Macrophage-Based Cell Therapeutics, ACS Appl Mater Interfaces , Vol. 15, Issue 44, pp. 50612–50625, doi:10.1021/acsami.2c22505., 2023 1944-8244 10.1021/acsami.2c22505. https://doi.org/10.1021/acsami.2c22505 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society (ACS) |
publisher.none.fl_str_mv |
American Chemical Society (ACS) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799137435991408640 |