Adhesive free-standing multilayer films containing sulfated levan for biomedical applications

Detalhes bibliográficos
Autor(a) principal: Gomes, Tiago D.
Data de Publicação: 2018
Outros Autores: Caridade, Sofia G., Sousa, Maria P., Azevedo, Sara, Kandur, Muhammed Y., Öner, Ebru T., Alves, Natália M., Mano, João F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/36719
Resumo: This work is the first reporting the use of layer-by-layer to produce adhesive free-standing (FS) films fully produced using natural-based macromolecules: chitosan (CHI), alginate (ALG) and sulfated levan (L-S). The deposition conditions of the natural polymers were studied through zeta potential measurements and quartz crystal microbalance with dissipation monitoring analysis. The properties of the FS films were evaluated and compared with the control ones composed of only CHI and ALG in order to assess the influence of levan polysaccharide introduced in the multilayers. Tensile tests, dynamic mechanical analysis and single lap shear strength tests were performed to evaluate the mechanical properties of the prepared FS films. The presence of L-S conferred both higher tensile strength and shear strength to the developed FS membranes. The results showed an adhesion strength 4 times higher than the control (CHI/ALG) FS films demonstrating the adhesive character of the FS films containing L-S. Morphological and topography studies were carried out revealing that the crosslinking reaction granted the L-S based FS film with a higher roughness and surface homogeneity. Preliminary biological assays were performed by cultivating myoblasts cells on the surface of the produced FS films. Both crosslinked and uncrosslinked FS films containing L-S were cytocompatible and myoconductive.
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spelling Adhesive free-standing multilayer films containing sulfated levan for biomedical applicationsSulfated levanFree-standing membranesAdhesivenessBiomedical applicationsThis work is the first reporting the use of layer-by-layer to produce adhesive free-standing (FS) films fully produced using natural-based macromolecules: chitosan (CHI), alginate (ALG) and sulfated levan (L-S). The deposition conditions of the natural polymers were studied through zeta potential measurements and quartz crystal microbalance with dissipation monitoring analysis. The properties of the FS films were evaluated and compared with the control ones composed of only CHI and ALG in order to assess the influence of levan polysaccharide introduced in the multilayers. Tensile tests, dynamic mechanical analysis and single lap shear strength tests were performed to evaluate the mechanical properties of the prepared FS films. The presence of L-S conferred both higher tensile strength and shear strength to the developed FS membranes. The results showed an adhesion strength 4 times higher than the control (CHI/ALG) FS films demonstrating the adhesive character of the FS films containing L-S. Morphological and topography studies were carried out revealing that the crosslinking reaction granted the L-S based FS film with a higher roughness and surface homogeneity. Preliminary biological assays were performed by cultivating myoblasts cells on the surface of the produced FS films. Both crosslinked and uncrosslinked FS films containing L-S were cytocompatible and myoconductive.2023-03-29T14:43:05Z2018-03-15T00:00:00Z2018-03-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/36719eng1742-706110.1016/j.actbio.2018.01.027Gomes, Tiago D.Caridade, Sofia G.Sousa, Maria P.Azevedo, SaraKandur, Muhammed Y.Öner, Ebru T.Alves, Natália M.Mano, João F.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:10:49Zoai:ria.ua.pt:10773/36719Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:07:26.925672Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Adhesive free-standing multilayer films containing sulfated levan for biomedical applications
title Adhesive free-standing multilayer films containing sulfated levan for biomedical applications
spellingShingle Adhesive free-standing multilayer films containing sulfated levan for biomedical applications
Gomes, Tiago D.
Sulfated levan
Free-standing membranes
Adhesiveness
Biomedical applications
title_short Adhesive free-standing multilayer films containing sulfated levan for biomedical applications
title_full Adhesive free-standing multilayer films containing sulfated levan for biomedical applications
title_fullStr Adhesive free-standing multilayer films containing sulfated levan for biomedical applications
title_full_unstemmed Adhesive free-standing multilayer films containing sulfated levan for biomedical applications
title_sort Adhesive free-standing multilayer films containing sulfated levan for biomedical applications
author Gomes, Tiago D.
author_facet Gomes, Tiago D.
Caridade, Sofia G.
Sousa, Maria P.
Azevedo, Sara
Kandur, Muhammed Y.
Öner, Ebru T.
Alves, Natália M.
Mano, João F.
author_role author
author2 Caridade, Sofia G.
Sousa, Maria P.
Azevedo, Sara
Kandur, Muhammed Y.
Öner, Ebru T.
Alves, Natália M.
Mano, João F.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gomes, Tiago D.
Caridade, Sofia G.
Sousa, Maria P.
Azevedo, Sara
Kandur, Muhammed Y.
Öner, Ebru T.
Alves, Natália M.
Mano, João F.
dc.subject.por.fl_str_mv Sulfated levan
Free-standing membranes
Adhesiveness
Biomedical applications
topic Sulfated levan
Free-standing membranes
Adhesiveness
Biomedical applications
description This work is the first reporting the use of layer-by-layer to produce adhesive free-standing (FS) films fully produced using natural-based macromolecules: chitosan (CHI), alginate (ALG) and sulfated levan (L-S). The deposition conditions of the natural polymers were studied through zeta potential measurements and quartz crystal microbalance with dissipation monitoring analysis. The properties of the FS films were evaluated and compared with the control ones composed of only CHI and ALG in order to assess the influence of levan polysaccharide introduced in the multilayers. Tensile tests, dynamic mechanical analysis and single lap shear strength tests were performed to evaluate the mechanical properties of the prepared FS films. The presence of L-S conferred both higher tensile strength and shear strength to the developed FS membranes. The results showed an adhesion strength 4 times higher than the control (CHI/ALG) FS films demonstrating the adhesive character of the FS films containing L-S. Morphological and topography studies were carried out revealing that the crosslinking reaction granted the L-S based FS film with a higher roughness and surface homogeneity. Preliminary biological assays were performed by cultivating myoblasts cells on the surface of the produced FS films. Both crosslinked and uncrosslinked FS films containing L-S were cytocompatible and myoconductive.
publishDate 2018
dc.date.none.fl_str_mv 2018-03-15T00:00:00Z
2018-03-15
2023-03-29T14:43:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/36719
url http://hdl.handle.net/10773/36719
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1742-7061
10.1016/j.actbio.2018.01.027
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dc.format.none.fl_str_mv application/pdf
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