Functional insights into the plant-specific SR45 splicing factor

Detalhes bibliográficos
Autor(a) principal: Nunes, Vera Alexandra Sacramento
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/8260
Resumo: Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia
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spelling Functional insights into the plant-specific SR45 splicing factor5PTase13ABAalternative splicingArabidopsisglucoseSR45Mestrado em Engenharia Agronómica - Instituto Superior de AgronomiaAlternative splicing is a post-transcriptional regulatory mechanism that generates transcriptome and proteome diversity. The SR protein family of splicing regulators is essential for the execution and regulation of both constitutive and alternative pre-mRNA splicing. The SR-related protein SR45 is an Arabidopsis bona fide splicing factor that plays a key role in plant development as well as in sugar and abscisic acid (ABA) signaling during early seedling development. The Arabidopsis 5PTase13, belonging to a group of inositol phosphatases involved in plant growth, development and stress responses, is a splicing target of SR45 previously implicated in hormonal and light signaling pathways. The 5PTase13 gene is alternatively spliced, generating two isoforms shown here to be ubiquitously expressed in Arabidopsis. The 5PTase13 loss-of-function mutant we isolated exhibits shorter roots when grown under low light conditions. Moreover, overexpression of either the 5PTase13.1 or the 5PTase13.2 isoforms in the sr45-1 background enhances the glucose hypersensitivity during early seedling development conferred by the sr45-1 mutation. Finally, confocal microscopy data suggests that both 5PTase13 isoforms are located in the cytoplasm, with 5PTase13.1 appearing mostly associated with the plasma membrane. This work provides new insight into the in vivo roles of the SR45 splicing factor and its functional connections to the 5PTase13 phosphataseISA/ULSantos, Paula Duque MagalhãesCarvalho, Raquel FonsecaAmâncio, Sara Barros QueirózRepositório da Universidade de LisboaNunes, Vera Alexandra Sacramento2015-03-20T10:19:08Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/8260TID:201921324engNunes, V.A.S. - Functional insights into the plant-specific SR45 splicing factor. Lisboa: ISA, 2014, 50 p.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:38:53Zoai:www.repository.utl.pt:10400.5/8260Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:55:19.388044Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Functional insights into the plant-specific SR45 splicing factor
title Functional insights into the plant-specific SR45 splicing factor
spellingShingle Functional insights into the plant-specific SR45 splicing factor
Nunes, Vera Alexandra Sacramento
5PTase13
ABA
alternative splicing
Arabidopsis
glucose
SR45
title_short Functional insights into the plant-specific SR45 splicing factor
title_full Functional insights into the plant-specific SR45 splicing factor
title_fullStr Functional insights into the plant-specific SR45 splicing factor
title_full_unstemmed Functional insights into the plant-specific SR45 splicing factor
title_sort Functional insights into the plant-specific SR45 splicing factor
author Nunes, Vera Alexandra Sacramento
author_facet Nunes, Vera Alexandra Sacramento
author_role author
dc.contributor.none.fl_str_mv Santos, Paula Duque Magalhães
Carvalho, Raquel Fonseca
Amâncio, Sara Barros Queiróz
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Nunes, Vera Alexandra Sacramento
dc.subject.por.fl_str_mv 5PTase13
ABA
alternative splicing
Arabidopsis
glucose
SR45
topic 5PTase13
ABA
alternative splicing
Arabidopsis
glucose
SR45
description Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
2015-03-20T10:19:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/8260
TID:201921324
url http://hdl.handle.net/10400.5/8260
identifier_str_mv TID:201921324
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nunes, V.A.S. - Functional insights into the plant-specific SR45 splicing factor. Lisboa: ISA, 2014, 50 p.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ISA/UL
publisher.none.fl_str_mv ISA/UL
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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