Kinetics of lactose fermentation using a recombinant saccharomyces cerevisiae strain
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/5864 |
Resumo: | This work presents a multi-route, non-structural kinetic model for interpretation of ethanol fermentation of lactose using a recombinant flocculent Saccharomyces cerevisiae strain expressing both the LAC4 (coding for β-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces lactis. In this model, the values of different metabolic pathways are calculated applying a modified Monod equation rate in which the growth rate is proportional to the concentration of a key enzyme controlling the single metabolic pathway. In this study, three main metabolic routes for S. cerevisiae are considered: oxidation of lactose, reduction of lactose (producing ethanol), and oxidation of ethanol. The main bioprocess variables determined experimentally were lactose, ethanol, biomass, and dissolved oxygen concentrations. Parameters of the proposed kinetic model were established by fitting the experimental data obtained in a small lab-scale fermentor with the initial lactose concentrations ranging from 5 g/dm³ to 50 g/dm³. A very good agreement between experimental data and simulated profiles of the main variables (lactose, ethanol, biomass, and dissolved oxygen concentrations) was achieved. |
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Kinetics of lactose fermentation using a recombinant saccharomyces cerevisiae strainEthanol fermentation of lactoseRecombinant saccharomyces cerevisiaeKinetic modelKinetic parameters estimationThis work presents a multi-route, non-structural kinetic model for interpretation of ethanol fermentation of lactose using a recombinant flocculent Saccharomyces cerevisiae strain expressing both the LAC4 (coding for β-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces lactis. In this model, the values of different metabolic pathways are calculated applying a modified Monod equation rate in which the growth rate is proportional to the concentration of a key enzyme controlling the single metabolic pathway. In this study, three main metabolic routes for S. cerevisiae are considered: oxidation of lactose, reduction of lactose (producing ethanol), and oxidation of ethanol. The main bioprocess variables determined experimentally were lactose, ethanol, biomass, and dissolved oxygen concentrations. Parameters of the proposed kinetic model were established by fitting the experimental data obtained in a small lab-scale fermentor with the initial lactose concentrations ranging from 5 g/dm³ to 50 g/dm³. A very good agreement between experimental data and simulated profiles of the main variables (lactose, ethanol, biomass, and dissolved oxygen concentrations) was achieved.Slovak Scientific Grant Agency ; Fundação para a Ciência e a Tecnologia (FCT)WileyUniversidade do MinhoJuraščík, MartinGuimarães, Pedro M. R.Klein, JaroslavDomingues, LucíliaTeixeira, J. A.Markoš, J.2006-082006-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/5864eng"Biotechnology and bioengineering". ISSN 0006-3592. 94:6 (Aug. 2006) 1147-1154.0006-359210.1002/bit.2094116615146info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:03:24Zoai:repositorium.sdum.uminho.pt:1822/5864Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:53:32.783300Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Kinetics of lactose fermentation using a recombinant saccharomyces cerevisiae strain |
title |
Kinetics of lactose fermentation using a recombinant saccharomyces cerevisiae strain |
spellingShingle |
Kinetics of lactose fermentation using a recombinant saccharomyces cerevisiae strain Juraščík, Martin Ethanol fermentation of lactose Recombinant saccharomyces cerevisiae Kinetic model Kinetic parameters estimation |
title_short |
Kinetics of lactose fermentation using a recombinant saccharomyces cerevisiae strain |
title_full |
Kinetics of lactose fermentation using a recombinant saccharomyces cerevisiae strain |
title_fullStr |
Kinetics of lactose fermentation using a recombinant saccharomyces cerevisiae strain |
title_full_unstemmed |
Kinetics of lactose fermentation using a recombinant saccharomyces cerevisiae strain |
title_sort |
Kinetics of lactose fermentation using a recombinant saccharomyces cerevisiae strain |
author |
Juraščík, Martin |
author_facet |
Juraščík, Martin Guimarães, Pedro M. R. Klein, Jaroslav Domingues, Lucília Teixeira, J. A. Markoš, J. |
author_role |
author |
author2 |
Guimarães, Pedro M. R. Klein, Jaroslav Domingues, Lucília Teixeira, J. A. Markoš, J. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Juraščík, Martin Guimarães, Pedro M. R. Klein, Jaroslav Domingues, Lucília Teixeira, J. A. Markoš, J. |
dc.subject.por.fl_str_mv |
Ethanol fermentation of lactose Recombinant saccharomyces cerevisiae Kinetic model Kinetic parameters estimation |
topic |
Ethanol fermentation of lactose Recombinant saccharomyces cerevisiae Kinetic model Kinetic parameters estimation |
description |
This work presents a multi-route, non-structural kinetic model for interpretation of ethanol fermentation of lactose using a recombinant flocculent Saccharomyces cerevisiae strain expressing both the LAC4 (coding for β-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces lactis. In this model, the values of different metabolic pathways are calculated applying a modified Monod equation rate in which the growth rate is proportional to the concentration of a key enzyme controlling the single metabolic pathway. In this study, three main metabolic routes for S. cerevisiae are considered: oxidation of lactose, reduction of lactose (producing ethanol), and oxidation of ethanol. The main bioprocess variables determined experimentally were lactose, ethanol, biomass, and dissolved oxygen concentrations. Parameters of the proposed kinetic model were established by fitting the experimental data obtained in a small lab-scale fermentor with the initial lactose concentrations ranging from 5 g/dm³ to 50 g/dm³. A very good agreement between experimental data and simulated profiles of the main variables (lactose, ethanol, biomass, and dissolved oxygen concentrations) was achieved. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-08 2006-08-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/5864 |
url |
http://hdl.handle.net/1822/5864 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Biotechnology and bioengineering". ISSN 0006-3592. 94:6 (Aug. 2006) 1147-1154. 0006-3592 10.1002/bit.20941 16615146 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132315260026880 |