Estimating pentose phosphate pathway activity from the analysis of hepatic glycogen 13 C-isotopomers derived from [U-13 C]fructose and [U-13 C]glucose

Detalhes bibliográficos
Autor(a) principal: Belew, Getachew D.
Data de Publicação: 2020
Outros Autores: Di Nunzio, Giada, Tavares, Ludgero, Silva, Joao G., Torres, Alejandra N., Jones, John Griffith
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/95082
https://doi.org/10.1002/mrm.28286
Resumo: The pentose phosphate pathway (PPP) is an important component of hepatic intermediary metabolism. Jin et al developed an elegant 13 C-NMR method for measuring hepatic PPP flux by quantifying the distribution of glucose 13 C-isotopomers formed from [U-13 C]glycerol. We demonstrate that this approach can be extended to exogenous [U-13 C]fructose and [U-13 C]glucose precursors by 13 C-NMR analysis of glycogen.
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spelling Estimating pentose phosphate pathway activity from the analysis of hepatic glycogen 13 C-isotopomers derived from [U-13 C]fructose and [U-13 C]glucoseNADPH; NMR; glucokinase; indirect pathwayAnimalsGlucoseMaleMiceMice, Inbred C57BLPentose Phosphate PathwayFructoseLiver GlycogenThe pentose phosphate pathway (PPP) is an important component of hepatic intermediary metabolism. Jin et al developed an elegant 13 C-NMR method for measuring hepatic PPP flux by quantifying the distribution of glucose 13 C-isotopomers formed from [U-13 C]glycerol. We demonstrate that this approach can be extended to exogenous [U-13 C]fructose and [U-13 C]glucose precursors by 13 C-NMR analysis of glycogen.2020-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/95082http://hdl.handle.net/10316/95082https://doi.org/10.1002/mrm.28286eng0740-31941522-2594https://onlinelibrary.wiley.com/doi/abs/10.1002/mrm.28286Belew, Getachew D.Di Nunzio, GiadaTavares, LudgeroSilva, Joao G.Torres, Alejandra N.Jones, John Griffithinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-11-03T10:50:32Zoai:estudogeral.uc.pt:10316/95082Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:13:39.943208Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Estimating pentose phosphate pathway activity from the analysis of hepatic glycogen 13 C-isotopomers derived from [U-13 C]fructose and [U-13 C]glucose
title Estimating pentose phosphate pathway activity from the analysis of hepatic glycogen 13 C-isotopomers derived from [U-13 C]fructose and [U-13 C]glucose
spellingShingle Estimating pentose phosphate pathway activity from the analysis of hepatic glycogen 13 C-isotopomers derived from [U-13 C]fructose and [U-13 C]glucose
Belew, Getachew D.
NADPH; NMR; glucokinase; indirect pathway
Animals
Glucose
Male
Mice
Mice, Inbred C57BL
Pentose Phosphate Pathway
Fructose
Liver Glycogen
title_short Estimating pentose phosphate pathway activity from the analysis of hepatic glycogen 13 C-isotopomers derived from [U-13 C]fructose and [U-13 C]glucose
title_full Estimating pentose phosphate pathway activity from the analysis of hepatic glycogen 13 C-isotopomers derived from [U-13 C]fructose and [U-13 C]glucose
title_fullStr Estimating pentose phosphate pathway activity from the analysis of hepatic glycogen 13 C-isotopomers derived from [U-13 C]fructose and [U-13 C]glucose
title_full_unstemmed Estimating pentose phosphate pathway activity from the analysis of hepatic glycogen 13 C-isotopomers derived from [U-13 C]fructose and [U-13 C]glucose
title_sort Estimating pentose phosphate pathway activity from the analysis of hepatic glycogen 13 C-isotopomers derived from [U-13 C]fructose and [U-13 C]glucose
author Belew, Getachew D.
author_facet Belew, Getachew D.
Di Nunzio, Giada
Tavares, Ludgero
Silva, Joao G.
Torres, Alejandra N.
Jones, John Griffith
author_role author
author2 Di Nunzio, Giada
Tavares, Ludgero
Silva, Joao G.
Torres, Alejandra N.
Jones, John Griffith
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Belew, Getachew D.
Di Nunzio, Giada
Tavares, Ludgero
Silva, Joao G.
Torres, Alejandra N.
Jones, John Griffith
dc.subject.por.fl_str_mv NADPH; NMR; glucokinase; indirect pathway
Animals
Glucose
Male
Mice
Mice, Inbred C57BL
Pentose Phosphate Pathway
Fructose
Liver Glycogen
topic NADPH; NMR; glucokinase; indirect pathway
Animals
Glucose
Male
Mice
Mice, Inbred C57BL
Pentose Phosphate Pathway
Fructose
Liver Glycogen
description The pentose phosphate pathway (PPP) is an important component of hepatic intermediary metabolism. Jin et al developed an elegant 13 C-NMR method for measuring hepatic PPP flux by quantifying the distribution of glucose 13 C-isotopomers formed from [U-13 C]glycerol. We demonstrate that this approach can be extended to exogenous [U-13 C]fructose and [U-13 C]glucose precursors by 13 C-NMR analysis of glycogen.
publishDate 2020
dc.date.none.fl_str_mv 2020-04
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/95082
http://hdl.handle.net/10316/95082
https://doi.org/10.1002/mrm.28286
url http://hdl.handle.net/10316/95082
https://doi.org/10.1002/mrm.28286
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0740-3194
1522-2594
https://onlinelibrary.wiley.com/doi/abs/10.1002/mrm.28286
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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