Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/11688 |
Resumo: | The optical dispersion and water content of human liver were experimentally studied to estimate the optical dispersions of tissue scatterers and dry matter. Using temporal measurements of collimated transmittance [Tcðt Þ] of liver samples under treatment at different glycerol concentrations, free water and diffusion coefficient (Dgl) of glycerol in liver were found as 60.0% and 8.2 × 10−7 cm2∕s, respectively. Bound water was calculated as the difference between the reported total water of 74.5% and found free water. The optical dispersion of liver was calculated from the measurements of refractive index (RI) of tissue samples made for different wavelengths between 400 and 1000 nm. Using liver and water optical dispersions at 20°C and the free and total water, the dispersions for liver scatterers and dry matter were calculated. The estimated dispersions present a decreasing behavior with wavelength. The dry matter dispersion shows higher RI values than liver scatterers, as expected. Considering 600 nm, dry matter has an RI of 1.508, whereas scatterers have an RI of 1.444. These dispersions are useful to characterize the RI matching mechanism in optical clearing treatments, provided that [Tcðt Þ] and thickness measurements are performed during treatment. The knowledge of Dgl is also important for living tissue cryoprotection applications. |
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Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissueHuman liver tissueRefractive indexFree and bound waterOptical clearingDiffusion coefficientCryoprotectionThe optical dispersion and water content of human liver were experimentally studied to estimate the optical dispersions of tissue scatterers and dry matter. Using temporal measurements of collimated transmittance [Tcðt Þ] of liver samples under treatment at different glycerol concentrations, free water and diffusion coefficient (Dgl) of glycerol in liver were found as 60.0% and 8.2 × 10−7 cm2∕s, respectively. Bound water was calculated as the difference between the reported total water of 74.5% and found free water. The optical dispersion of liver was calculated from the measurements of refractive index (RI) of tissue samples made for different wavelengths between 400 and 1000 nm. Using liver and water optical dispersions at 20°C and the free and total water, the dispersions for liver scatterers and dry matter were calculated. The estimated dispersions present a decreasing behavior with wavelength. The dry matter dispersion shows higher RI values than liver scatterers, as expected. Considering 600 nm, dry matter has an RI of 1.508, whereas scatterers have an RI of 1.444. These dispersions are useful to characterize the RI matching mechanism in optical clearing treatments, provided that [Tcðt Þ] and thickness measurements are performed during treatment. The knowledge of Dgl is also important for living tissue cryoprotection applications.Society of Photo-optical Instrumentation EngineersRepositório Científico do Instituto Politécnico do PortoCarneiro, IsaCarvalho, SóniaHenrique, RuiOliveira, LuísTuchin, Valery2017-12-052119-01-01T00:00:00Z2017-12-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/11688eng10.1117/1.JBO.22.12.125002metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:52:04Zoai:recipp.ipp.pt:10400.22/11688Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:30:57.781508Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue |
title |
Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue |
spellingShingle |
Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue Carneiro, Isa Human liver tissue Refractive index Free and bound water Optical clearing Diffusion coefficient Cryoprotection |
title_short |
Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue |
title_full |
Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue |
title_fullStr |
Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue |
title_full_unstemmed |
Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue |
title_sort |
Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue |
author |
Carneiro, Isa |
author_facet |
Carneiro, Isa Carvalho, Sónia Henrique, Rui Oliveira, Luís Tuchin, Valery |
author_role |
author |
author2 |
Carvalho, Sónia Henrique, Rui Oliveira, Luís Tuchin, Valery |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Carneiro, Isa Carvalho, Sónia Henrique, Rui Oliveira, Luís Tuchin, Valery |
dc.subject.por.fl_str_mv |
Human liver tissue Refractive index Free and bound water Optical clearing Diffusion coefficient Cryoprotection |
topic |
Human liver tissue Refractive index Free and bound water Optical clearing Diffusion coefficient Cryoprotection |
description |
The optical dispersion and water content of human liver were experimentally studied to estimate the optical dispersions of tissue scatterers and dry matter. Using temporal measurements of collimated transmittance [Tcðt Þ] of liver samples under treatment at different glycerol concentrations, free water and diffusion coefficient (Dgl) of glycerol in liver were found as 60.0% and 8.2 × 10−7 cm2∕s, respectively. Bound water was calculated as the difference between the reported total water of 74.5% and found free water. The optical dispersion of liver was calculated from the measurements of refractive index (RI) of tissue samples made for different wavelengths between 400 and 1000 nm. Using liver and water optical dispersions at 20°C and the free and total water, the dispersions for liver scatterers and dry matter were calculated. The estimated dispersions present a decreasing behavior with wavelength. The dry matter dispersion shows higher RI values than liver scatterers, as expected. Considering 600 nm, dry matter has an RI of 1.508, whereas scatterers have an RI of 1.444. These dispersions are useful to characterize the RI matching mechanism in optical clearing treatments, provided that [Tcðt Þ] and thickness measurements are performed during treatment. The knowledge of Dgl is also important for living tissue cryoprotection applications. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-05 2017-12-05T00:00:00Z 2119-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/11688 |
url |
http://hdl.handle.net/10400.22/11688 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1117/1.JBO.22.12.125002 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Society of Photo-optical Instrumentation Engineers |
publisher.none.fl_str_mv |
Society of Photo-optical Instrumentation Engineers |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799131405409583104 |