Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials

Detalhes bibliográficos
Autor(a) principal: Lopes, Joana
Data de Publicação: 2020
Outros Autores: Coelho, João Miguel Pinto, Vieira, Pedro Manuel Cardoso, Viana, Ana Silveira, Gaspar, Maria Manuela, Reis, Catarina Pinto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/52298
Resumo: Supplementary Materials - The following are available online at https://www.mdpi.com/2079-4991/10/8/1536/s1, Video S1: Thermal activation of HAOA-coated GNPs.
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spelling Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterialsmelanomagold nanoparticlesbioproductionlaser photothermal therapyin vitro modelsexperimental resultsSupplementary Materials - The following are available online at https://www.mdpi.com/2079-4991/10/8/1536/s1, Video S1: Thermal activation of HAOA-coated GNPs.Cancer like melanoma is a complex disease, for which standard therapies have significant adverse side effects that in most cases are ineffective and highly unspecific. Thus, a new paradigm has come with the need of achieving alternative (less invasive) and effective therapies. In this work, biocompatible gold nanoparticles (GNPs) coated with hyaluronic acid and oleic acid were prepared and characterized in terms of size, morphology and cytotoxicity in the presence of Saccharomyces cerevisiae, and two cell lines, the keratinocytes (healthy skin cells, HaCat) and the melanoma cells (B16F10). Results showed that these GNPs absorb within the near-infrared region (750–1400 nm), in the optical therapeutic window (from 650 to 1300 nm), in contrast to other commercial gold nanoparticles, which enables light to penetrate into deep skin layers. A laser emitting in this region was applied and its effect also analyzed. The coated GNPs showed a spherical morphology with a mean size of 297 nm without cytotoxic effects towards yeast and tested cell lines. Nevertheless, after laser irradiation, a reduction of 20% in B16F10 cell line viability was observed. In summary, this work appears to be a promising strategy for the treatment of non-metastatic melanoma or other superficial tumors.This research was funded by Fundação para a Ciência e a Tecnologia (FCT) through the projects: UIDB/00645/2020, UID/DTP/04138/2019, PTDC/MED-QUI/31721/2017 as well as by the PhD fellowship SFRH/BD/148044/2019 (J.L.).Repositório da Universidade de LisboaLopes, JoanaCoelho, João Miguel PintoVieira, Pedro Manuel CardosoViana, Ana SilveiraGaspar, Maria ManuelaReis, Catarina Pinto2022-04-12T12:33:24Z2020-08-052022-02-22T10:53:55Z2020-08-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/52298engLopes J, Coelho JMP, Vieira PMC, Viana AS, Gaspar MM, Reis C. Preliminary assays towards melanoma cells using phototherapy with gold-based nanomaterials. Nanomaterials [Internet]. 2020;10(8):1536. Disponível em: https://www.mdpi.com/2079-4991/10/8/15362079-4991cv-prod-2446145https://doi.org/10.3390/nano10081536info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:56:08Zoai:repositorio.ul.pt:10451/52298Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:02:44.186324Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials
title Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials
spellingShingle Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials
Lopes, Joana
melanoma
gold nanoparticles
bioproduction
laser photothermal therapy
in vitro models
experimental results
title_short Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials
title_full Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials
title_fullStr Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials
title_full_unstemmed Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials
title_sort Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials
author Lopes, Joana
author_facet Lopes, Joana
Coelho, João Miguel Pinto
Vieira, Pedro Manuel Cardoso
Viana, Ana Silveira
Gaspar, Maria Manuela
Reis, Catarina Pinto
author_role author
author2 Coelho, João Miguel Pinto
Vieira, Pedro Manuel Cardoso
Viana, Ana Silveira
Gaspar, Maria Manuela
Reis, Catarina Pinto
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Lopes, Joana
Coelho, João Miguel Pinto
Vieira, Pedro Manuel Cardoso
Viana, Ana Silveira
Gaspar, Maria Manuela
Reis, Catarina Pinto
dc.subject.por.fl_str_mv melanoma
gold nanoparticles
bioproduction
laser photothermal therapy
in vitro models
experimental results
topic melanoma
gold nanoparticles
bioproduction
laser photothermal therapy
in vitro models
experimental results
description Supplementary Materials - The following are available online at https://www.mdpi.com/2079-4991/10/8/1536/s1, Video S1: Thermal activation of HAOA-coated GNPs.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-05
2020-08-05T00:00:00Z
2022-04-12T12:33:24Z
2022-02-22T10:53:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/52298
url http://hdl.handle.net/10451/52298
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lopes J, Coelho JMP, Vieira PMC, Viana AS, Gaspar MM, Reis C. Preliminary assays towards melanoma cells using phototherapy with gold-based nanomaterials. Nanomaterials [Internet]. 2020;10(8):1536. Disponível em: https://www.mdpi.com/2079-4991/10/8/1536
2079-4991
cv-prod-2446145
https://doi.org/10.3390/nano10081536
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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