Characterization of the rhipicephalus (Boophilus) microplus sialotranscriptome profile in response to theileria equi infection

Detalhes bibliográficos
Autor(a) principal: Paulino, Patrícia
Data de Publicação: 2021
Outros Autores: Vitari, Gabriela, Rezende, Antonio, Couto, Joana, Antunes, Sandra, Domingos, Ana, Peckle, Maristela, Massard, Carlos, Araújo, Flávio, Santos, Huarrisson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/130684
Resumo: Funding Information: Funding: The authors disclose receipt of the following financial support for the research, authorship, or publication of this paper. The National Council for Scientific and Technological Development and the ’Carlos Chagas Filho’ Foundation for Research Support of the State of Rio de Janeiro provided financial support for this study. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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spelling Characterization of the rhipicephalus (Boophilus) microplus sialotranscriptome profile in response to theileria equi infectionEquine piroplasmosisRNA-seqTick defenseTick-protozoan interactionsImmunology and AllergyMolecular BiologyImmunology and Microbiology(all)Microbiology (medical)Infectious DiseasesSDG 3 - Good Health and Well-beingSDG 15 - Life on LandFunding Information: Funding: The authors disclose receipt of the following financial support for the research, authorship, or publication of this paper. The National Council for Scientific and Technological Development and the ’Carlos Chagas Filho’ Foundation for Research Support of the State of Rio de Janeiro provided financial support for this study. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.This study intends to characterize the sialotranscriptome profile of Rhipicephalus (Boophilus) microplus in response to Theileria equi and identify genes of interest with differential genomic ex-pression, indicating relevant targets in the tick–protozoan interactions. The experimental design consisted of RNA sequencing from uninfected and T. equi-infected R. microplus salivary glands (SGs) to obtain transcriptomic profiles for characterization and comparison. A total of 288,952 transcripts were obtained from both tick profiles, 3456 transcripts (p < 0.05) differentially expressed in response to T. equi infection. The uninfected SGs’ registered 231,179 transcripts, of which 155,359 were annotated. The most transcribed sequences were female-specific histamine binding protein and lipocalins. Regarding the T. equi-infected SGs, from the 238,964 assembled transcripts, 163,564 were annotated. The most transcribed sequences were histone demethylase JARID1 and Y-box-binding protein. Five transcripts (cystatin, arginase, nuclear factor κB kinase inhibitor subunit β (IκB), IκB delta, lysosomal-trafficking regulator, and reeler protein) presented the gene ontology (GO) category “response to protozoan” and were exclusively displayed in the T. equi-infected profile. The tran-scriptome of T. equi was also analyzed, registering 4728 hits. The study’s genetic and molecular information would be of great value for future studies and biotechnological applications envisaging disease control.Instituto de Higiene e Medicina Tropical (IHMT)Vector borne diseases and pathogens (VBD)Global Health and Tropical Medicine (GHTM)RUNPaulino, PatríciaVitari, GabrielaRezende, AntonioCouto, JoanaAntunes, SandraDomingos, AnaPeckle, MaristelaMassard, CarlosAraújo, FlávioSantos, Huarrisson2022-01-12T04:09:52Z2021-022021-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article18application/pdfhttp://hdl.handle.net/10362/130684eng2076-0817PURE: 33022906https://doi.org/10.3390/pathogens10020167info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:09:17Zoai:run.unl.pt:10362/130684Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:46:50.935995Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Characterization of the rhipicephalus (Boophilus) microplus sialotranscriptome profile in response to theileria equi infection
title Characterization of the rhipicephalus (Boophilus) microplus sialotranscriptome profile in response to theileria equi infection
spellingShingle Characterization of the rhipicephalus (Boophilus) microplus sialotranscriptome profile in response to theileria equi infection
Paulino, Patrícia
Equine piroplasmosis
RNA-seq
Tick defense
Tick-protozoan interactions
Immunology and Allergy
Molecular Biology
Immunology and Microbiology(all)
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
SDG 15 - Life on Land
title_short Characterization of the rhipicephalus (Boophilus) microplus sialotranscriptome profile in response to theileria equi infection
title_full Characterization of the rhipicephalus (Boophilus) microplus sialotranscriptome profile in response to theileria equi infection
title_fullStr Characterization of the rhipicephalus (Boophilus) microplus sialotranscriptome profile in response to theileria equi infection
title_full_unstemmed Characterization of the rhipicephalus (Boophilus) microplus sialotranscriptome profile in response to theileria equi infection
title_sort Characterization of the rhipicephalus (Boophilus) microplus sialotranscriptome profile in response to theileria equi infection
author Paulino, Patrícia
author_facet Paulino, Patrícia
Vitari, Gabriela
Rezende, Antonio
Couto, Joana
Antunes, Sandra
Domingos, Ana
Peckle, Maristela
Massard, Carlos
Araújo, Flávio
Santos, Huarrisson
author_role author
author2 Vitari, Gabriela
Rezende, Antonio
Couto, Joana
Antunes, Sandra
Domingos, Ana
Peckle, Maristela
Massard, Carlos
Araújo, Flávio
Santos, Huarrisson
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Higiene e Medicina Tropical (IHMT)
Vector borne diseases and pathogens (VBD)
Global Health and Tropical Medicine (GHTM)
RUN
dc.contributor.author.fl_str_mv Paulino, Patrícia
Vitari, Gabriela
Rezende, Antonio
Couto, Joana
Antunes, Sandra
Domingos, Ana
Peckle, Maristela
Massard, Carlos
Araújo, Flávio
Santos, Huarrisson
dc.subject.por.fl_str_mv Equine piroplasmosis
RNA-seq
Tick defense
Tick-protozoan interactions
Immunology and Allergy
Molecular Biology
Immunology and Microbiology(all)
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
SDG 15 - Life on Land
topic Equine piroplasmosis
RNA-seq
Tick defense
Tick-protozoan interactions
Immunology and Allergy
Molecular Biology
Immunology and Microbiology(all)
Microbiology (medical)
Infectious Diseases
SDG 3 - Good Health and Well-being
SDG 15 - Life on Land
description Funding Information: Funding: The authors disclose receipt of the following financial support for the research, authorship, or publication of this paper. The National Council for Scientific and Technological Development and the ’Carlos Chagas Filho’ Foundation for Research Support of the State of Rio de Janeiro provided financial support for this study. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2021
dc.date.none.fl_str_mv 2021-02
2021-02-01T00:00:00Z
2022-01-12T04:09:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/130684
url http://hdl.handle.net/10362/130684
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2076-0817
PURE: 33022906
https://doi.org/10.3390/pathogens10020167
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 18
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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