Sphaerococcus coronopifolius bromoterpenes as potential cancer stem cell-targeting agents
Main Author: | |
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Publication Date: | 2020 |
Other Authors: | , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/10400.8/5112 |
Summary: | Acknowledgments This work was supported by the Portuguese Foundation for Science and Technology (FCT) through strategic project UID/MAR/04292/ 2020 and UID/Multi/04046/2020 granted to MARE—Marine and Environmental Sciences Centre and BioISI—BioSystems and Integrative Sciences Institute, respectively, through Red2Discovery project (PTDC/ MAR-BIO/6149/2014), co-financed by COMPETE (POCI-01-0145FEDER-016791), through Oncologia de Precisão: Terapias e Tecnologias Inovadoras project (POINT4PAC) (SAICTPAC/0019/2015LISBOA-01-0145-FEDER-016405) and through CROSS-ATLANTIC project (PTDC/BIA-OUT/29250/2017), co-financed by COMPETE (POCI01-0145-FEDER-029250). This work was also funded by the Integrated Programme of SR&TD "Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate" (reference Centro-010145-FEDER-000018), co-funded by Centro 2020 Programme, Portugal 2020, European Union, through the European Regional Development Fund. FCT is also acknowledged for the grant attributed to J.S. (SFRH/ BD/103255/2014). |
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Sphaerococcus coronopifolius bromoterpenes as potential cancer stem cell-targeting agentsMarine natural productsRed seaweedLung cancerCancer stem cellsMicroenvironmentInterleukin-6Acknowledgments This work was supported by the Portuguese Foundation for Science and Technology (FCT) through strategic project UID/MAR/04292/ 2020 and UID/Multi/04046/2020 granted to MARE—Marine and Environmental Sciences Centre and BioISI—BioSystems and Integrative Sciences Institute, respectively, through Red2Discovery project (PTDC/ MAR-BIO/6149/2014), co-financed by COMPETE (POCI-01-0145FEDER-016791), through Oncologia de Precisão: Terapias e Tecnologias Inovadoras project (POINT4PAC) (SAICTPAC/0019/2015LISBOA-01-0145-FEDER-016405) and through CROSS-ATLANTIC project (PTDC/BIA-OUT/29250/2017), co-financed by COMPETE (POCI01-0145-FEDER-029250). This work was also funded by the Integrated Programme of SR&TD "Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate" (reference Centro-010145-FEDER-000018), co-funded by Centro 2020 Programme, Portugal 2020, European Union, through the European Regional Development Fund. FCT is also acknowledged for the grant attributed to J.S. (SFRH/ BD/103255/2014).Cancer is one of the major threats to human health and, due to distinct factors, it is expected that its incidence will increase in the next decades leading to an urgent need of new anticancer drugs development. Ongoing experimental and clinical observations propose that cancer cells with stem-like properties (CSCs) are involved on the development of lung cancer chemoresistance. As tumour growth and metastasis can be controlled by tumourassociated stromal cells, the main goal of this study was to access the antitumor potential of five bromoterpenes isolated from Sphaerococcus coronopifolius red alga to target CSCs originated in a co-culture system of fibroblast and lung malignant cells. Cytotoxicity of compounds (10–500 μM; 72 h) was evaluated on monocultures of several malignant and non-malignant cells lines (HBF, BEAS-2B, RenG2, SC-DRenG2) and the effects estimated by MTT assay. Co-cultures of non-malignant human bronchial fibroblasts (HBF) and malignant human bronchial epithelial cells (RenG2) were implemented and the compounds ability to selectively kill CSCs was evaluated by sphere forming assay. The interleucine-6 (IL-6) levels were also determined as cytokine is crucial for CSCs. Regarding the monocultures results bromosphaerol selectively eliminated the malignant cells. Both 12S-hydroxy-bromosphaerol and 12R-hydroxy-bromosphaerol steroisomers were cytotoxic towards non-malignant bronchial BEAS-2B cell line, IC50 of 4.29 and 4.30 μM respectively. However, none of the steroisomers induced damage in the HBFs. As to the co-cultures, 12R-hydroxy-bromosphaerol revealed the highest cytotoxicity and ability to abrogate the malignant stem cells; however its effects were IL-6 independent. The results presented here are the first evidence of the potential of these bromoterpenes to abrogate CSCs opening new research opportunities. The 12R-hydroxy-bromosphaerol revealed to be the most promising compound to be test in more complex living models.ElsevierIC-OnlineAlves, CelsoSerrano, EuricoSilva, JoanaRodrigues, CarlosPinteus, SuseteGaspar, HelenaBotana, Luis M.Alpoim, Maria C.Pedrosa, Rui2020-08-28T11:11:09Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/5112engAlves, C., Serrano, E., Silva, J., Rodrigues, C., Pinteus, S., Gaspar, H., Botana, L., Alpoim, M., & Pedrosa, Rui. (2020). Sphaerococcus coronopifolius bromoterpenes as potential cancer stem cell-targeting agents. Biomedicine & Pharmacotherapy. 128. DOI: 10.1016/j.biopha.2020.110275.0753-332210.1016/j.biopha.2020.110275metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-26T18:18:27Zoai:iconline.ipleiria.pt:10400.8/5112Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-26T18:18:27Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Sphaerococcus coronopifolius bromoterpenes as potential cancer stem cell-targeting agents |
title |
Sphaerococcus coronopifolius bromoterpenes as potential cancer stem cell-targeting agents |
spellingShingle |
Sphaerococcus coronopifolius bromoterpenes as potential cancer stem cell-targeting agents Alves, Celso Marine natural products Red seaweed Lung cancer Cancer stem cells Microenvironment Interleukin-6 |
title_short |
Sphaerococcus coronopifolius bromoterpenes as potential cancer stem cell-targeting agents |
title_full |
Sphaerococcus coronopifolius bromoterpenes as potential cancer stem cell-targeting agents |
title_fullStr |
Sphaerococcus coronopifolius bromoterpenes as potential cancer stem cell-targeting agents |
title_full_unstemmed |
Sphaerococcus coronopifolius bromoterpenes as potential cancer stem cell-targeting agents |
title_sort |
Sphaerococcus coronopifolius bromoterpenes as potential cancer stem cell-targeting agents |
author |
Alves, Celso |
author_facet |
Alves, Celso Serrano, Eurico Silva, Joana Rodrigues, Carlos Pinteus, Susete Gaspar, Helena Botana, Luis M. Alpoim, Maria C. Pedrosa, Rui |
author_role |
author |
author2 |
Serrano, Eurico Silva, Joana Rodrigues, Carlos Pinteus, Susete Gaspar, Helena Botana, Luis M. Alpoim, Maria C. Pedrosa, Rui |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
IC-Online |
dc.contributor.author.fl_str_mv |
Alves, Celso Serrano, Eurico Silva, Joana Rodrigues, Carlos Pinteus, Susete Gaspar, Helena Botana, Luis M. Alpoim, Maria C. Pedrosa, Rui |
dc.subject.por.fl_str_mv |
Marine natural products Red seaweed Lung cancer Cancer stem cells Microenvironment Interleukin-6 |
topic |
Marine natural products Red seaweed Lung cancer Cancer stem cells Microenvironment Interleukin-6 |
description |
Acknowledgments This work was supported by the Portuguese Foundation for Science and Technology (FCT) through strategic project UID/MAR/04292/ 2020 and UID/Multi/04046/2020 granted to MARE—Marine and Environmental Sciences Centre and BioISI—BioSystems and Integrative Sciences Institute, respectively, through Red2Discovery project (PTDC/ MAR-BIO/6149/2014), co-financed by COMPETE (POCI-01-0145FEDER-016791), through Oncologia de Precisão: Terapias e Tecnologias Inovadoras project (POINT4PAC) (SAICTPAC/0019/2015LISBOA-01-0145-FEDER-016405) and through CROSS-ATLANTIC project (PTDC/BIA-OUT/29250/2017), co-financed by COMPETE (POCI01-0145-FEDER-029250). This work was also funded by the Integrated Programme of SR&TD "Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate" (reference Centro-010145-FEDER-000018), co-funded by Centro 2020 Programme, Portugal 2020, European Union, through the European Regional Development Fund. FCT is also acknowledged for the grant attributed to J.S. (SFRH/ BD/103255/2014). |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-28T11:11:09Z 2020 2020-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.8/5112 |
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http://hdl.handle.net/10400.8/5112 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Alves, C., Serrano, E., Silva, J., Rodrigues, C., Pinteus, S., Gaspar, H., Botana, L., Alpoim, M., & Pedrosa, Rui. (2020). Sphaerococcus coronopifolius bromoterpenes as potential cancer stem cell-targeting agents. Biomedicine & Pharmacotherapy. 128. DOI: 10.1016/j.biopha.2020.110275. 0753-3322 10.1016/j.biopha.2020.110275 |
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metadata only access info:eu-repo/semantics/openAccess |
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metadata only access |
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openAccess |
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application/pdf |
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Elsevier |
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Elsevier |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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