The interaction between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum

Detalhes bibliográficos
Autor(a) principal: WALEWSKA, Edyta
Data de Publicação: 2019
Outros Autores: WOŁODKO, Karolina M., SKARZYNSKI, Dariusz, Ferreira-Dias, Graça, Galvão, António
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/22446
Resumo: Research Areas: Endocrinology & Metabolism
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spelling The interaction between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus LuteumNodalhypoxia inducible factor 1 alphathrombospondin 1prostaglandin F2 alphacorpus luteumluteolysisResearch Areas: Endocrinology & MetabolismThe regulation of corpus luteus (CL) luteolysis is a complex process involving a myriad of factors. Previously, we have shown the involvement of Nodal in functional luteolysis in mares. Presently, we ask the extent of which Nodal mediation of luteolysis is done through regulation of angioregression. We demonstrated the interaction between Nodal and hypoxia-inducible factor 1 a (HIF1a) and thrombospondin 1/thrombospondin receptor (TSP1/CD36) systems, could mediate angioregression during luteolysis. First, we demonstrated the inhibitory effect of Nodal on the vascularmarker platelet/endothelial cell adhesion molecule 1 (CD31). Also, treatment of mid CL explants with vascular endothelial growth factor A (VEGFA) showed a trend on activin-like kinase 7 (Alk7) protein inhibition. Next, Nodal was also shown to activate HIF1a and in vitro culture of mid CL explants under decreased oxygen level promoted Nodal expression and SMAD familymember 3 (Smad3) phosphorylation. In another experiment, the crosstalk between Nodal and TSP1/CD36 was investigated. Indeed, Nodal increased the expression of the anti-angiogenic TSP1 and its receptor CD36 in mid CL explants. Finally, the supportive effect of prostaglandin F2a (PGF2a) on TSP1/CD36 was blocked by SB431542 (SB), a pharmacological inhibitor of Nodal signaling. Thus, we evidenced for the first time the in vitro interaction between Nodal and both HIF1a and TSP1 systems, two conserved pathways previously shown to be involved in vascular regression during luteolysis. Considering the given increased expression of Nodal in mid CL and its role on functional luteolysis, the current results suggest the additional involvement of Nodal in angioregression during luteolysis in the mare, particularly in the activation of HIF1a and TSP1/CD36.Lane K. Christenson, University of Kansas Medical Center, United StatesRepositório da Universidade de LisboaWALEWSKA, EdytaWOŁODKO, Karolina M.SKARZYNSKI, DariuszFerreira-Dias, GraçaGalvão, António2021-10-28T23:07:13Z2019-10-012019-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22446engWalewska E, Wołodko K, Skarzynski D, Ferreira-Dias G, Galvão A. 2019. The interaction between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum. Frontiers in Endocrinology 10:667. doi: 10.3389/fendo.2019.006671664-239210.3389/fendo.2019.00667info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:51:58Zoai:www.repository.utl.pt:10400.5/22446Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:52.265610Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The interaction between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum
title The interaction between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum
spellingShingle The interaction between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum
WALEWSKA, Edyta
Nodal
hypoxia inducible factor 1 alpha
thrombospondin 1
prostaglandin F2 alpha
corpus luteum
luteolysis
title_short The interaction between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum
title_full The interaction between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum
title_fullStr The interaction between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum
title_full_unstemmed The interaction between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum
title_sort The interaction between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum
author WALEWSKA, Edyta
author_facet WALEWSKA, Edyta
WOŁODKO, Karolina M.
SKARZYNSKI, Dariusz
Ferreira-Dias, Graça
Galvão, António
author_role author
author2 WOŁODKO, Karolina M.
SKARZYNSKI, Dariusz
Ferreira-Dias, Graça
Galvão, António
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv WALEWSKA, Edyta
WOŁODKO, Karolina M.
SKARZYNSKI, Dariusz
Ferreira-Dias, Graça
Galvão, António
dc.subject.por.fl_str_mv Nodal
hypoxia inducible factor 1 alpha
thrombospondin 1
prostaglandin F2 alpha
corpus luteum
luteolysis
topic Nodal
hypoxia inducible factor 1 alpha
thrombospondin 1
prostaglandin F2 alpha
corpus luteum
luteolysis
description Research Areas: Endocrinology & Metabolism
publishDate 2019
dc.date.none.fl_str_mv 2019-10-01
2019-10-01T00:00:00Z
2021-10-28T23:07:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/22446
url http://hdl.handle.net/10400.5/22446
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Walewska E, Wołodko K, Skarzynski D, Ferreira-Dias G, Galvão A. 2019. The interaction between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum. Frontiers in Endocrinology 10:667. doi: 10.3389/fendo.2019.00667
1664-2392
10.3389/fendo.2019.00667
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Lane K. Christenson, University of Kansas Medical Center, United States
publisher.none.fl_str_mv Lane K. Christenson, University of Kansas Medical Center, United States
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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