Control of somite number/size in zebrafish upon reduction of progenitor cells

Detalhes bibliográficos
Autor(a) principal: Carvalho, Lara Margarida Marques Saraiva de
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/3556
Resumo: Dissertação de mest., Biologia Marinha, Faculdade de Ciências e Tecnologia, Univ. do Algarve, 2012
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spelling Control of somite number/size in zebrafish upon reduction of progenitor cellsBiologia marinhaPeixesPeixe zebraDesenvolvimentoDissertação de mest., Biologia Marinha, Faculdade de Ciências e Tecnologia, Univ. do Algarve, 2012Somites are transient embryonic structures, formed in a sequential and rhythmic manner from the presomitic mesoderm (PSM) in a process termed somitogenesis. The somites are precursors of the repetitive structures of vertebrates: the vertebral column with their ribs and associated skeletal muscles. Each new pair of somites is generated from the PSM in a sequentially and rhythmic manner: a new pair of somites is formed every 30 minutes in the zebrafish, 2 hours in the mouse and 4-5 hours in humans. The total number of somites is also species specific and varies from ~30 in humans and zebrafish, to ~60 in mouse. Amazingly, embryos where large portions of PSM progenitor cells, at the blastula stage or later stages of development, were physically removed develop into smaller embryos, yet they form the same number of somites (Cooke, 1975). These experiments illustrate the regulative capacity and robustness of somite formation and lead to the clock and wavefront model of somitogenesis (Cooke and Zeeeman, 1975). A plethora of molecular and genetic evidences have emerged to support the clock and wavefront model. However, how the embryos regulate the total somite number has not been so much explored. Therefore, we set out to re-visit the work of Cooke (1975) using new genetic tools available in zebrafish, where it is possible to reduce the size of the mesoderm progenitor population genetically at different time-points of development and access its impact on somite number and size. We made use of heat-shock transgenic lines hsp70:msgn1, hsp70:dkk1:GFP and hsp70:fgfdn:GFP – that allowed us to modulate the mesoderm progenitor population upon a heat-shocked during gastrulation, trunk or tail development by interfering with the levels of Mesogenin1, Wnt and Fgf signalling, respectively. We conclude that Wnt signalling plays a role not only in the regulation of the total somite number but also in regulating somite size.Fior, RitaPalmeirim, IsabelSapientiaCarvalho, Lara Margarida Marques Saraiva de2014-03-04T14:31:02Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.1/3556enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:14:38Zoai:sapientia.ualg.pt:10400.1/3556Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:57:05.438425Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Control of somite number/size in zebrafish upon reduction of progenitor cells
title Control of somite number/size in zebrafish upon reduction of progenitor cells
spellingShingle Control of somite number/size in zebrafish upon reduction of progenitor cells
Carvalho, Lara Margarida Marques Saraiva de
Biologia marinha
Peixes
Peixe zebra
Desenvolvimento
title_short Control of somite number/size in zebrafish upon reduction of progenitor cells
title_full Control of somite number/size in zebrafish upon reduction of progenitor cells
title_fullStr Control of somite number/size in zebrafish upon reduction of progenitor cells
title_full_unstemmed Control of somite number/size in zebrafish upon reduction of progenitor cells
title_sort Control of somite number/size in zebrafish upon reduction of progenitor cells
author Carvalho, Lara Margarida Marques Saraiva de
author_facet Carvalho, Lara Margarida Marques Saraiva de
author_role author
dc.contributor.none.fl_str_mv Fior, Rita
Palmeirim, Isabel
Sapientia
dc.contributor.author.fl_str_mv Carvalho, Lara Margarida Marques Saraiva de
dc.subject.por.fl_str_mv Biologia marinha
Peixes
Peixe zebra
Desenvolvimento
topic Biologia marinha
Peixes
Peixe zebra
Desenvolvimento
description Dissertação de mest., Biologia Marinha, Faculdade de Ciências e Tecnologia, Univ. do Algarve, 2012
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2014-03-04T14:31:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/3556
url http://hdl.handle.net/10400.1/3556
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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