Bone injury and repair trigger central and peripheral NPY Neuronal Pathways
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/13745 |
Resumo: | Bone repair is a specialized type of wound repair controlled by complex multi-factorial events. The nervous system is recognized as one of the key regulators of bone mass, thereby suggesting a role for neuronal pathways in bone homeostasis. However, in the context of bone injury and repair, little is known on the interplay between the nervous system and bone. Here, we addressed the neuropeptide Y (NPY) neuronal arm during the initial stages of bone repair encompassing the inflammatory response and ossification phases in femoral-defect mouse model. Spatial and temporal analysis of transcriptional and protein levels of NPY and its receptors, Y1R and Y2R, reported to be involved in bone homeostasis, was performed in bone, dorsal root ganglia (DRG) and hypothalamus after femoral injury. The results showed that NPY system activity is increased in a time- and space-dependent manner during bone repair. Y1R expression was trigged in both bone and DRG throughout the inflammatory phase, while a Y2R response was restricted to the hypothalamus and at a later stage, during the ossification step. Our results provide new insights into the involvement of NPY neuronal pathways in bone repair. |
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Bone injury and repair trigger central and peripheral NPY Neuronal PathwaysAnimalsFemurGanglia, SpinalGene Expression RegulationHypothalamusMaleMiceMice, Inbred C57BLNeural PathwaysNeuropeptide YRNA, MessengerReceptors, Neuropeptide YSignal TransductionWound HealingBone repair is a specialized type of wound repair controlled by complex multi-factorial events. The nervous system is recognized as one of the key regulators of bone mass, thereby suggesting a role for neuronal pathways in bone homeostasis. However, in the context of bone injury and repair, little is known on the interplay between the nervous system and bone. Here, we addressed the neuropeptide Y (NPY) neuronal arm during the initial stages of bone repair encompassing the inflammatory response and ossification phases in femoral-defect mouse model. Spatial and temporal analysis of transcriptional and protein levels of NPY and its receptors, Y1R and Y2R, reported to be involved in bone homeostasis, was performed in bone, dorsal root ganglia (DRG) and hypothalamus after femoral injury. The results showed that NPY system activity is increased in a time- and space-dependent manner during bone repair. Y1R expression was trigged in both bone and DRG throughout the inflammatory phase, while a Y2R response was restricted to the hypothalamus and at a later stage, during the ossification step. Our results provide new insights into the involvement of NPY neuronal pathways in bone repair.Repositório Científico do Instituto Politécnico do PortoAlves, Cecília J.Alencastre, Inês S.Neto, EstrelaRibas, JoãoFerreira, SofiaVasconcelos, Daniel M.Sousa, Daniela M.Summavielle, TeresaLamghari, Meriem2019-05-20T12:49:46Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/13745engAlves, C. J., Alencastre, I. S., Neto, E., Ribas, J., Ferreira, S., Vasconcelos, D. M., Sousa, D. M., Summavielle, T., & Lamghari, M. (2016). Bone Injury and Repair Trigger Central and Peripheral NPY Neuronal Pathways. PLoS ONE, 11(11), e0165465. https://doi.org/10.1371/journal.pone.01654651932-620310.1371/journal.pone.0165465info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-20T01:53:14Zoai:recipp.ipp.pt:10400.22/13745Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:33:36.888321Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bone injury and repair trigger central and peripheral NPY Neuronal Pathways |
title |
Bone injury and repair trigger central and peripheral NPY Neuronal Pathways |
spellingShingle |
Bone injury and repair trigger central and peripheral NPY Neuronal Pathways Alves, Cecília J. Animals Femur Ganglia, Spinal Gene Expression Regulation Hypothalamus Male Mice Mice, Inbred C57BL Neural Pathways Neuropeptide Y RNA, Messenger Receptors, Neuropeptide Y Signal Transduction Wound Healing |
title_short |
Bone injury and repair trigger central and peripheral NPY Neuronal Pathways |
title_full |
Bone injury and repair trigger central and peripheral NPY Neuronal Pathways |
title_fullStr |
Bone injury and repair trigger central and peripheral NPY Neuronal Pathways |
title_full_unstemmed |
Bone injury and repair trigger central and peripheral NPY Neuronal Pathways |
title_sort |
Bone injury and repair trigger central and peripheral NPY Neuronal Pathways |
author |
Alves, Cecília J. |
author_facet |
Alves, Cecília J. Alencastre, Inês S. Neto, Estrela Ribas, João Ferreira, Sofia Vasconcelos, Daniel M. Sousa, Daniela M. Summavielle, Teresa Lamghari, Meriem |
author_role |
author |
author2 |
Alencastre, Inês S. Neto, Estrela Ribas, João Ferreira, Sofia Vasconcelos, Daniel M. Sousa, Daniela M. Summavielle, Teresa Lamghari, Meriem |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Alves, Cecília J. Alencastre, Inês S. Neto, Estrela Ribas, João Ferreira, Sofia Vasconcelos, Daniel M. Sousa, Daniela M. Summavielle, Teresa Lamghari, Meriem |
dc.subject.por.fl_str_mv |
Animals Femur Ganglia, Spinal Gene Expression Regulation Hypothalamus Male Mice Mice, Inbred C57BL Neural Pathways Neuropeptide Y RNA, Messenger Receptors, Neuropeptide Y Signal Transduction Wound Healing |
topic |
Animals Femur Ganglia, Spinal Gene Expression Regulation Hypothalamus Male Mice Mice, Inbred C57BL Neural Pathways Neuropeptide Y RNA, Messenger Receptors, Neuropeptide Y Signal Transduction Wound Healing |
description |
Bone repair is a specialized type of wound repair controlled by complex multi-factorial events. The nervous system is recognized as one of the key regulators of bone mass, thereby suggesting a role for neuronal pathways in bone homeostasis. However, in the context of bone injury and repair, little is known on the interplay between the nervous system and bone. Here, we addressed the neuropeptide Y (NPY) neuronal arm during the initial stages of bone repair encompassing the inflammatory response and ossification phases in femoral-defect mouse model. Spatial and temporal analysis of transcriptional and protein levels of NPY and its receptors, Y1R and Y2R, reported to be involved in bone homeostasis, was performed in bone, dorsal root ganglia (DRG) and hypothalamus after femoral injury. The results showed that NPY system activity is increased in a time- and space-dependent manner during bone repair. Y1R expression was trigged in both bone and DRG throughout the inflammatory phase, while a Y2R response was restricted to the hypothalamus and at a later stage, during the ossification step. Our results provide new insights into the involvement of NPY neuronal pathways in bone repair. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z 2019-05-20T12:49:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/13745 |
url |
http://hdl.handle.net/10400.22/13745 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Alves, C. J., Alencastre, I. S., Neto, E., Ribas, J., Ferreira, S., Vasconcelos, D. M., Sousa, D. M., Summavielle, T., & Lamghari, M. (2016). Bone Injury and Repair Trigger Central and Peripheral NPY Neuronal Pathways. PLoS ONE, 11(11), e0165465. https://doi.org/10.1371/journal.pone.0165465 1932-6203 10.1371/journal.pone.0165465 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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