Competence for Natural Transformation Is Common among Clinical Strains of Resistant Acinetobacter spp

Detalhes bibliográficos
Autor(a) principal: Domingues, Sara
Data de Publicação: 2019
Outros Autores: Rosário, Natasha, Cândido, Ângela, Neto, Daniela, Nielsen, Kaare M, Silva, Gabriela J. da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/107164
https://doi.org/10.3390/microorganisms7020030
Resumo: Horizontal gene transfer events provide the basis for extensive dissemination of antimicrobial resistance traits between bacterial populations. Conjugation is considered to be the most frequent mechanism behind new resistance acquisitions in clinical pathogens but does not fully explain the resistance patterns seen in some bacterial genera. Gene transfer by natural transformation has been described for numerous clinical isolates, including some Acinetobacter species. The main aim of this study was to determine to what extent clinical, resistant Acinetobacter spp. isolates, express competence for natural transformation. Twenty-two clinical Acinetobacter spp. isolates collected over a 16-year time period, from five different geographical separated and/or distinct Portuguese Hospitals were tested for natural transformability. Fourteen isolates, including 11 A. baumannii, 2 A. nosocomialis and 1 Acinetobacter sp., were identified as competent on semisolid media facilitating surface-motility. Competent Acinetobacter isolates were found in all the hospitals tested. Furthermore, osmolarity was shown to influence the uptake of exogenous DNA by competent A. baumannii A118. Our study demonstrates that natural competence is common among clinical isolates of Acinetobacter spp., and hence likely an important trait for resistance acquisition.
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spelling Competence for Natural Transformation Is Common among Clinical Strains of Resistant Acinetobacter sppAcinetobacter baumanniiAcinetobacter nosocomialisclinical isolateshorizontal gene transfernatural transformationcompetenceosmolarityHorizontal gene transfer events provide the basis for extensive dissemination of antimicrobial resistance traits between bacterial populations. Conjugation is considered to be the most frequent mechanism behind new resistance acquisitions in clinical pathogens but does not fully explain the resistance patterns seen in some bacterial genera. Gene transfer by natural transformation has been described for numerous clinical isolates, including some Acinetobacter species. The main aim of this study was to determine to what extent clinical, resistant Acinetobacter spp. isolates, express competence for natural transformation. Twenty-two clinical Acinetobacter spp. isolates collected over a 16-year time period, from five different geographical separated and/or distinct Portuguese Hospitals were tested for natural transformability. Fourteen isolates, including 11 A. baumannii, 2 A. nosocomialis and 1 Acinetobacter sp., were identified as competent on semisolid media facilitating surface-motility. Competent Acinetobacter isolates were found in all the hospitals tested. Furthermore, osmolarity was shown to influence the uptake of exogenous DNA by competent A. baumannii A118. Our study demonstrates that natural competence is common among clinical isolates of Acinetobacter spp., and hence likely an important trait for resistance acquisition.MDPI2019-01-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/107164http://hdl.handle.net/10316/107164https://doi.org/10.3390/microorganisms7020030eng2076-2607Domingues, SaraRosário, NatashaCândido, ÂngelaNeto, DanielaNielsen, Kaare MSilva, Gabriela J. dainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-06-12T11:35:38Zoai:estudogeral.uc.pt:10316/107164Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:23:31.506407Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Competence for Natural Transformation Is Common among Clinical Strains of Resistant Acinetobacter spp
title Competence for Natural Transformation Is Common among Clinical Strains of Resistant Acinetobacter spp
spellingShingle Competence for Natural Transformation Is Common among Clinical Strains of Resistant Acinetobacter spp
Domingues, Sara
Acinetobacter baumannii
Acinetobacter nosocomialis
clinical isolates
horizontal gene transfer
natural transformation
competence
osmolarity
title_short Competence for Natural Transformation Is Common among Clinical Strains of Resistant Acinetobacter spp
title_full Competence for Natural Transformation Is Common among Clinical Strains of Resistant Acinetobacter spp
title_fullStr Competence for Natural Transformation Is Common among Clinical Strains of Resistant Acinetobacter spp
title_full_unstemmed Competence for Natural Transformation Is Common among Clinical Strains of Resistant Acinetobacter spp
title_sort Competence for Natural Transformation Is Common among Clinical Strains of Resistant Acinetobacter spp
author Domingues, Sara
author_facet Domingues, Sara
Rosário, Natasha
Cândido, Ângela
Neto, Daniela
Nielsen, Kaare M
Silva, Gabriela J. da
author_role author
author2 Rosário, Natasha
Cândido, Ângela
Neto, Daniela
Nielsen, Kaare M
Silva, Gabriela J. da
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Domingues, Sara
Rosário, Natasha
Cândido, Ângela
Neto, Daniela
Nielsen, Kaare M
Silva, Gabriela J. da
dc.subject.por.fl_str_mv Acinetobacter baumannii
Acinetobacter nosocomialis
clinical isolates
horizontal gene transfer
natural transformation
competence
osmolarity
topic Acinetobacter baumannii
Acinetobacter nosocomialis
clinical isolates
horizontal gene transfer
natural transformation
competence
osmolarity
description Horizontal gene transfer events provide the basis for extensive dissemination of antimicrobial resistance traits between bacterial populations. Conjugation is considered to be the most frequent mechanism behind new resistance acquisitions in clinical pathogens but does not fully explain the resistance patterns seen in some bacterial genera. Gene transfer by natural transformation has been described for numerous clinical isolates, including some Acinetobacter species. The main aim of this study was to determine to what extent clinical, resistant Acinetobacter spp. isolates, express competence for natural transformation. Twenty-two clinical Acinetobacter spp. isolates collected over a 16-year time period, from five different geographical separated and/or distinct Portuguese Hospitals were tested for natural transformability. Fourteen isolates, including 11 A. baumannii, 2 A. nosocomialis and 1 Acinetobacter sp., were identified as competent on semisolid media facilitating surface-motility. Competent Acinetobacter isolates were found in all the hospitals tested. Furthermore, osmolarity was shown to influence the uptake of exogenous DNA by competent A. baumannii A118. Our study demonstrates that natural competence is common among clinical isolates of Acinetobacter spp., and hence likely an important trait for resistance acquisition.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-24
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/107164
http://hdl.handle.net/10316/107164
https://doi.org/10.3390/microorganisms7020030
url http://hdl.handle.net/10316/107164
https://doi.org/10.3390/microorganisms7020030
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