Biocompatibility of a self-assembled crosslinkable hyaluronic acid nanogel

Detalhes bibliográficos
Autor(a) principal: Pedrosa, Sílvia Santos
Data de Publicação: 2016
Outros Autores: Pereira, Paula Alexandra Cunha, Correia, Alexandra, Moreira, Susana, Rocha, Huho, Gama, F. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/43001
Resumo: Hyaluronic acid nanogel (HyA-AT) is a redox sensitive crosslinkable nanogel, obtained through the conjugation of a thiolated hydrophobic molecule to the hyaluronic acid chain. Engineered nanogel was studied for its biocompatibility, including immunocompatibility and hemocompatability. The nanogel did not compromise the metabolic activity or cellular membrane integrity of 3T3, microvascular endothelial cells, and RAW 264.7 cell lines, as determined by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide and lactate dehydrogenase release assays. Also, we didn't observe any apoptotic effect on these cell lines through the Annexin V-FITC test. Furthermore, the nanogel cell internalization was analyzed using murine bone marrow derived macrophages, and the in vivo and ex vivo biodistribution of the Cy5.5 labeled nanogel was monitored using a non-invasive near-infrared fluorescence imaging system. The HyA-AT nanogel exhibits fairly a long half-live in the blood stream, thus showing potential for drug delivery applications.
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spelling Biocompatibility of a self-assembled crosslinkable hyaluronic acid nanogelBiocompatibilityBiodistributionHyaluronic acidImmunocompatibilityNanogelScience & TechnologyHyaluronic acid nanogel (HyA-AT) is a redox sensitive crosslinkable nanogel, obtained through the conjugation of a thiolated hydrophobic molecule to the hyaluronic acid chain. Engineered nanogel was studied for its biocompatibility, including immunocompatibility and hemocompatability. The nanogel did not compromise the metabolic activity or cellular membrane integrity of 3T3, microvascular endothelial cells, and RAW 264.7 cell lines, as determined by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide and lactate dehydrogenase release assays. Also, we didn't observe any apoptotic effect on these cell lines through the Annexin V-FITC test. Furthermore, the nanogel cell internalization was analyzed using murine bone marrow derived macrophages, and the in vivo and ex vivo biodistribution of the Cy5.5 labeled nanogel was monitored using a non-invasive near-infrared fluorescence imaging system. The HyA-AT nanogel exhibits fairly a long half-live in the blood stream, thus showing potential for drug delivery applications.The authors thank the FCT Strategic Project of UID/BIO/04469/2013 unit, the project RECI/ BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462) and the Project “BioHealth – Biotechnology and Bioengineering approaches to improve health quality”, Ref. NORTE-07-0124- FEDER-000027, co-funded by the Programa Operacional Regional do Norte (ON.2 – O Novo Norte), QREN, FEDER. The authors would like to acknowledge also the support of FCT for the PhD grant reference SFRH/BD/61516/2009. They would also like to thank Bioimaging department on Molecular Medicine Institute (IMM) in Lisbon, namely Dr José Rino and Dr António Temudo. Also thank the animal facilities in IMM (Lisbon), specially Dr. Dolores Bonaparte and Dr. Joana Marques. Finally, the authors thank Dr Africa Gonzalez and Mercedes Pelletero the performance of the studies on the activation of complement.John Wiley and SonsUniversidade do MinhoPedrosa, Sílvia SantosPereira, Paula Alexandra CunhaCorreia, AlexandraMoreira, SusanaRocha, HuhoGama, F. M.20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/43001engPedrosa, Sílvia S.; Pereira, Paula; Correia, Alexandre; Moreira, Susana; Rocha, H.; Gama, F. M., Biocompatibility of a self-assembled crosslinkable hyaluronic acid nanogel. Macromolecular Bioscience, 16(11), 1610-1620, 20161616-51871616-519510.1002/mabi.20160022127456215http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1616-5195info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:00:59Zoai:repositorium.sdum.uminho.pt:1822/43001Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:50:53.985924Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biocompatibility of a self-assembled crosslinkable hyaluronic acid nanogel
title Biocompatibility of a self-assembled crosslinkable hyaluronic acid nanogel
spellingShingle Biocompatibility of a self-assembled crosslinkable hyaluronic acid nanogel
Pedrosa, Sílvia Santos
Biocompatibility
Biodistribution
Hyaluronic acid
Immunocompatibility
Nanogel
Science & Technology
title_short Biocompatibility of a self-assembled crosslinkable hyaluronic acid nanogel
title_full Biocompatibility of a self-assembled crosslinkable hyaluronic acid nanogel
title_fullStr Biocompatibility of a self-assembled crosslinkable hyaluronic acid nanogel
title_full_unstemmed Biocompatibility of a self-assembled crosslinkable hyaluronic acid nanogel
title_sort Biocompatibility of a self-assembled crosslinkable hyaluronic acid nanogel
author Pedrosa, Sílvia Santos
author_facet Pedrosa, Sílvia Santos
Pereira, Paula Alexandra Cunha
Correia, Alexandra
Moreira, Susana
Rocha, Huho
Gama, F. M.
author_role author
author2 Pereira, Paula Alexandra Cunha
Correia, Alexandra
Moreira, Susana
Rocha, Huho
Gama, F. M.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pedrosa, Sílvia Santos
Pereira, Paula Alexandra Cunha
Correia, Alexandra
Moreira, Susana
Rocha, Huho
Gama, F. M.
dc.subject.por.fl_str_mv Biocompatibility
Biodistribution
Hyaluronic acid
Immunocompatibility
Nanogel
Science & Technology
topic Biocompatibility
Biodistribution
Hyaluronic acid
Immunocompatibility
Nanogel
Science & Technology
description Hyaluronic acid nanogel (HyA-AT) is a redox sensitive crosslinkable nanogel, obtained through the conjugation of a thiolated hydrophobic molecule to the hyaluronic acid chain. Engineered nanogel was studied for its biocompatibility, including immunocompatibility and hemocompatability. The nanogel did not compromise the metabolic activity or cellular membrane integrity of 3T3, microvascular endothelial cells, and RAW 264.7 cell lines, as determined by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide and lactate dehydrogenase release assays. Also, we didn't observe any apoptotic effect on these cell lines through the Annexin V-FITC test. Furthermore, the nanogel cell internalization was analyzed using murine bone marrow derived macrophages, and the in vivo and ex vivo biodistribution of the Cy5.5 labeled nanogel was monitored using a non-invasive near-infrared fluorescence imaging system. The HyA-AT nanogel exhibits fairly a long half-live in the blood stream, thus showing potential for drug delivery applications.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/43001
url http://hdl.handle.net/1822/43001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pedrosa, Sílvia S.; Pereira, Paula; Correia, Alexandre; Moreira, Susana; Rocha, H.; Gama, F. M., Biocompatibility of a self-assembled crosslinkable hyaluronic acid nanogel. Macromolecular Bioscience, 16(11), 1610-1620, 2016
1616-5187
1616-5195
10.1002/mabi.201600221
27456215
http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1616-5195
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley and Sons
publisher.none.fl_str_mv John Wiley and Sons
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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