Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagy
Autor(a) principal: | |
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Data de Publicação: | 2024 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/43998 |
Resumo: | Blueberries, red fruits enriched in polyphenols and fibers, are envisaged as a promising nutraceutical intervention in a plethora of metabolic diseases. Prediabetes, an intermediate state between normal glucose tolerance and type 2 diabetes, fuels the development of complications, including hepatic steatosis. In previous work, we have demonstrated that blueberry juice (BJ) supplementation benefits glycemic control and lipid profile, which was accompanied by an amelioration of hepatic mitochondrial bioenergetics. The purpose of this study is to clarify the impact of long-term BJ nutraceutical intervention on cellular mechanisms that govern hepatic lipid homeostasis, namely autophagy and endoplasmic reticulum (ER) stress, in a rat model of prediabetes. Two groups of male Wistar rats, 8-weeks old, were fed a prediabetes-inducing high-fat diet (HFD) and one group was fed a control diet (CD). From the timepoint where the prediabetic phenotype was achieved (week 16) until the end of the study (week 24), one of the HFD-fed groups was daily orally supplemented with 25 g/kg body weight (BW) of BJ (HFD + BJ). BW, caloric intake, glucose tolerance and insulin sensitivity were monitored throughout the study. The serum and hepatic lipid contents were quantified. Liver and interscapular brown and epidydimal white adipose tissue depots (iBAT and eWAT) were collected for histological analysis and to assess thermogenesis, ER stress and autophagy markers. The gut microbiota composition and the short-chain fatty acids (SCFAs) content were determined in colon fecal samples. BJ supplementation positively impacted glycemic control but was unable to prevent obesity and adiposity. BJ-treated animals presented a reduction in fecal SCFAs, increased markers of arrested iBAT thermogenesis and energy expenditure, together with an aggravation of HFD-induced lipotoxicity and hepatic steatosis, which were accompanied by the inhibition of autophagy and ER stress responses in the liver. In conclusion, despite the improvement of glucose tolerance, BJ supplementation promoted a major impact on lipid management mechanisms at liver and AT levels in prediabetic animals, which might affect disease course. |
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Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagyBlueberry supplementationPrediabetesDiet-induced rat modelHepatic endoplasmic stress response and autophagyBlueberries, red fruits enriched in polyphenols and fibers, are envisaged as a promising nutraceutical intervention in a plethora of metabolic diseases. Prediabetes, an intermediate state between normal glucose tolerance and type 2 diabetes, fuels the development of complications, including hepatic steatosis. In previous work, we have demonstrated that blueberry juice (BJ) supplementation benefits glycemic control and lipid profile, which was accompanied by an amelioration of hepatic mitochondrial bioenergetics. The purpose of this study is to clarify the impact of long-term BJ nutraceutical intervention on cellular mechanisms that govern hepatic lipid homeostasis, namely autophagy and endoplasmic reticulum (ER) stress, in a rat model of prediabetes. Two groups of male Wistar rats, 8-weeks old, were fed a prediabetes-inducing high-fat diet (HFD) and one group was fed a control diet (CD). From the timepoint where the prediabetic phenotype was achieved (week 16) until the end of the study (week 24), one of the HFD-fed groups was daily orally supplemented with 25 g/kg body weight (BW) of BJ (HFD + BJ). BW, caloric intake, glucose tolerance and insulin sensitivity were monitored throughout the study. The serum and hepatic lipid contents were quantified. Liver and interscapular brown and epidydimal white adipose tissue depots (iBAT and eWAT) were collected for histological analysis and to assess thermogenesis, ER stress and autophagy markers. The gut microbiota composition and the short-chain fatty acids (SCFAs) content were determined in colon fecal samples. BJ supplementation positively impacted glycemic control but was unable to prevent obesity and adiposity. BJ-treated animals presented a reduction in fecal SCFAs, increased markers of arrested iBAT thermogenesis and energy expenditure, together with an aggravation of HFD-induced lipotoxicity and hepatic steatosis, which were accompanied by the inhibition of autophagy and ER stress responses in the liver. In conclusion, despite the improvement of glucose tolerance, BJ supplementation promoted a major impact on lipid management mechanisms at liver and AT levels in prediabetic animals, which might affect disease course.Veritati - Repositório Institucional da Universidade Católica PortuguesaFerreira, GonçaloVieira, PedroAlves, AndréNunes, SaraPreguiça, InêsMartins-Marques, TâniaRibeiro, TâniaGirão, HenriqueFigueirinha, ArturSalgueiro, LígiaPintado, ManuelaGomes, PedroViana, SofiaReis, Flávio2024-02-16T12:22:56Z2024-02-132024-02-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/43998eng2072-664310.3390/nu160405138518594439638398840info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-12T01:38:33Zoai:repositorio.ucp.pt:10400.14/43998Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:39:03.153901Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagy |
title |
Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagy |
spellingShingle |
Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagy Ferreira, Gonçalo Blueberry supplementation Prediabetes Diet-induced rat model Hepatic endoplasmic stress response and autophagy |
title_short |
Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagy |
title_full |
Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagy |
title_fullStr |
Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagy |
title_full_unstemmed |
Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagy |
title_sort |
Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagy |
author |
Ferreira, Gonçalo |
author_facet |
Ferreira, Gonçalo Vieira, Pedro Alves, André Nunes, Sara Preguiça, Inês Martins-Marques, Tânia Ribeiro, Tânia Girão, Henrique Figueirinha, Artur Salgueiro, Lígia Pintado, Manuela Gomes, Pedro Viana, Sofia Reis, Flávio |
author_role |
author |
author2 |
Vieira, Pedro Alves, André Nunes, Sara Preguiça, Inês Martins-Marques, Tânia Ribeiro, Tânia Girão, Henrique Figueirinha, Artur Salgueiro, Lígia Pintado, Manuela Gomes, Pedro Viana, Sofia Reis, Flávio |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Ferreira, Gonçalo Vieira, Pedro Alves, André Nunes, Sara Preguiça, Inês Martins-Marques, Tânia Ribeiro, Tânia Girão, Henrique Figueirinha, Artur Salgueiro, Lígia Pintado, Manuela Gomes, Pedro Viana, Sofia Reis, Flávio |
dc.subject.por.fl_str_mv |
Blueberry supplementation Prediabetes Diet-induced rat model Hepatic endoplasmic stress response and autophagy |
topic |
Blueberry supplementation Prediabetes Diet-induced rat model Hepatic endoplasmic stress response and autophagy |
description |
Blueberries, red fruits enriched in polyphenols and fibers, are envisaged as a promising nutraceutical intervention in a plethora of metabolic diseases. Prediabetes, an intermediate state between normal glucose tolerance and type 2 diabetes, fuels the development of complications, including hepatic steatosis. In previous work, we have demonstrated that blueberry juice (BJ) supplementation benefits glycemic control and lipid profile, which was accompanied by an amelioration of hepatic mitochondrial bioenergetics. The purpose of this study is to clarify the impact of long-term BJ nutraceutical intervention on cellular mechanisms that govern hepatic lipid homeostasis, namely autophagy and endoplasmic reticulum (ER) stress, in a rat model of prediabetes. Two groups of male Wistar rats, 8-weeks old, were fed a prediabetes-inducing high-fat diet (HFD) and one group was fed a control diet (CD). From the timepoint where the prediabetic phenotype was achieved (week 16) until the end of the study (week 24), one of the HFD-fed groups was daily orally supplemented with 25 g/kg body weight (BW) of BJ (HFD + BJ). BW, caloric intake, glucose tolerance and insulin sensitivity were monitored throughout the study. The serum and hepatic lipid contents were quantified. Liver and interscapular brown and epidydimal white adipose tissue depots (iBAT and eWAT) were collected for histological analysis and to assess thermogenesis, ER stress and autophagy markers. The gut microbiota composition and the short-chain fatty acids (SCFAs) content were determined in colon fecal samples. BJ supplementation positively impacted glycemic control but was unable to prevent obesity and adiposity. BJ-treated animals presented a reduction in fecal SCFAs, increased markers of arrested iBAT thermogenesis and energy expenditure, together with an aggravation of HFD-induced lipotoxicity and hepatic steatosis, which were accompanied by the inhibition of autophagy and ER stress responses in the liver. In conclusion, despite the improvement of glucose tolerance, BJ supplementation promoted a major impact on lipid management mechanisms at liver and AT levels in prediabetic animals, which might affect disease course. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-02-16T12:22:56Z 2024-02-13 2024-02-13T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/43998 |
url |
http://hdl.handle.net/10400.14/43998 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2072-6643 10.3390/nu16040513 85185944396 38398840 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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