Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagy

Detalhes bibliográficos
Autor(a) principal: Ferreira, Gonçalo
Data de Publicação: 2024
Outros Autores: Vieira, Pedro, Alves, André, Nunes, Sara, Preguiça, Inês, Martins-Marques, Tânia, Ribeiro, Tânia, Girão, Henrique, Figueirinha, Artur, Salgueiro, Lígia, Pintado, Manuela, Gomes, Pedro, Viana, Sofia, Reis, Flávio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/43998
Resumo: Blueberries, red fruits enriched in polyphenols and fibers, are envisaged as a promising nutraceutical intervention in a plethora of metabolic diseases. Prediabetes, an intermediate state between normal glucose tolerance and type 2 diabetes, fuels the development of complications, including hepatic steatosis. In previous work, we have demonstrated that blueberry juice (BJ) supplementation benefits glycemic control and lipid profile, which was accompanied by an amelioration of hepatic mitochondrial bioenergetics. The purpose of this study is to clarify the impact of long-term BJ nutraceutical intervention on cellular mechanisms that govern hepatic lipid homeostasis, namely autophagy and endoplasmic reticulum (ER) stress, in a rat model of prediabetes. Two groups of male Wistar rats, 8-weeks old, were fed a prediabetes-inducing high-fat diet (HFD) and one group was fed a control diet (CD). From the timepoint where the prediabetic phenotype was achieved (week 16) until the end of the study (week 24), one of the HFD-fed groups was daily orally supplemented with 25 g/kg body weight (BW) of BJ (HFD + BJ). BW, caloric intake, glucose tolerance and insulin sensitivity were monitored throughout the study. The serum and hepatic lipid contents were quantified. Liver and interscapular brown and epidydimal white adipose tissue depots (iBAT and eWAT) were collected for histological analysis and to assess thermogenesis, ER stress and autophagy markers. The gut microbiota composition and the short-chain fatty acids (SCFAs) content were determined in colon fecal samples. BJ supplementation positively impacted glycemic control but was unable to prevent obesity and adiposity. BJ-treated animals presented a reduction in fecal SCFAs, increased markers of arrested iBAT thermogenesis and energy expenditure, together with an aggravation of HFD-induced lipotoxicity and hepatic steatosis, which were accompanied by the inhibition of autophagy and ER stress responses in the liver. In conclusion, despite the improvement of glucose tolerance, BJ supplementation promoted a major impact on lipid management mechanisms at liver and AT levels in prediabetic animals, which might affect disease course.
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spelling Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagyBlueberry supplementationPrediabetesDiet-induced rat modelHepatic endoplasmic stress response and autophagyBlueberries, red fruits enriched in polyphenols and fibers, are envisaged as a promising nutraceutical intervention in a plethora of metabolic diseases. Prediabetes, an intermediate state between normal glucose tolerance and type 2 diabetes, fuels the development of complications, including hepatic steatosis. In previous work, we have demonstrated that blueberry juice (BJ) supplementation benefits glycemic control and lipid profile, which was accompanied by an amelioration of hepatic mitochondrial bioenergetics. The purpose of this study is to clarify the impact of long-term BJ nutraceutical intervention on cellular mechanisms that govern hepatic lipid homeostasis, namely autophagy and endoplasmic reticulum (ER) stress, in a rat model of prediabetes. Two groups of male Wistar rats, 8-weeks old, were fed a prediabetes-inducing high-fat diet (HFD) and one group was fed a control diet (CD). From the timepoint where the prediabetic phenotype was achieved (week 16) until the end of the study (week 24), one of the HFD-fed groups was daily orally supplemented with 25 g/kg body weight (BW) of BJ (HFD + BJ). BW, caloric intake, glucose tolerance and insulin sensitivity were monitored throughout the study. The serum and hepatic lipid contents were quantified. Liver and interscapular brown and epidydimal white adipose tissue depots (iBAT and eWAT) were collected for histological analysis and to assess thermogenesis, ER stress and autophagy markers. The gut microbiota composition and the short-chain fatty acids (SCFAs) content were determined in colon fecal samples. BJ supplementation positively impacted glycemic control but was unable to prevent obesity and adiposity. BJ-treated animals presented a reduction in fecal SCFAs, increased markers of arrested iBAT thermogenesis and energy expenditure, together with an aggravation of HFD-induced lipotoxicity and hepatic steatosis, which were accompanied by the inhibition of autophagy and ER stress responses in the liver. In conclusion, despite the improvement of glucose tolerance, BJ supplementation promoted a major impact on lipid management mechanisms at liver and AT levels in prediabetic animals, which might affect disease course.Veritati - Repositório Institucional da Universidade Católica PortuguesaFerreira, GonçaloVieira, PedroAlves, AndréNunes, SaraPreguiça, InêsMartins-Marques, TâniaRibeiro, TâniaGirão, HenriqueFigueirinha, ArturSalgueiro, LígiaPintado, ManuelaGomes, PedroViana, SofiaReis, Flávio2024-02-16T12:22:56Z2024-02-132024-02-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/43998eng2072-664310.3390/nu160405138518594439638398840info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-12T01:38:33Zoai:repositorio.ucp.pt:10400.14/43998Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:39:03.153901Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagy
title Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagy
spellingShingle Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagy
Ferreira, Gonçalo
Blueberry supplementation
Prediabetes
Diet-induced rat model
Hepatic endoplasmic stress response and autophagy
title_short Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagy
title_full Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagy
title_fullStr Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagy
title_full_unstemmed Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagy
title_sort Effect of blueberry supplementation on a diet-induced rat model of prediabetes—focus on hepatic lipid deposition, endoplasmic stress response and autophagy
author Ferreira, Gonçalo
author_facet Ferreira, Gonçalo
Vieira, Pedro
Alves, André
Nunes, Sara
Preguiça, Inês
Martins-Marques, Tânia
Ribeiro, Tânia
Girão, Henrique
Figueirinha, Artur
Salgueiro, Lígia
Pintado, Manuela
Gomes, Pedro
Viana, Sofia
Reis, Flávio
author_role author
author2 Vieira, Pedro
Alves, André
Nunes, Sara
Preguiça, Inês
Martins-Marques, Tânia
Ribeiro, Tânia
Girão, Henrique
Figueirinha, Artur
Salgueiro, Lígia
Pintado, Manuela
Gomes, Pedro
Viana, Sofia
Reis, Flávio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Ferreira, Gonçalo
Vieira, Pedro
Alves, André
Nunes, Sara
Preguiça, Inês
Martins-Marques, Tânia
Ribeiro, Tânia
Girão, Henrique
Figueirinha, Artur
Salgueiro, Lígia
Pintado, Manuela
Gomes, Pedro
Viana, Sofia
Reis, Flávio
dc.subject.por.fl_str_mv Blueberry supplementation
Prediabetes
Diet-induced rat model
Hepatic endoplasmic stress response and autophagy
topic Blueberry supplementation
Prediabetes
Diet-induced rat model
Hepatic endoplasmic stress response and autophagy
description Blueberries, red fruits enriched in polyphenols and fibers, are envisaged as a promising nutraceutical intervention in a plethora of metabolic diseases. Prediabetes, an intermediate state between normal glucose tolerance and type 2 diabetes, fuels the development of complications, including hepatic steatosis. In previous work, we have demonstrated that blueberry juice (BJ) supplementation benefits glycemic control and lipid profile, which was accompanied by an amelioration of hepatic mitochondrial bioenergetics. The purpose of this study is to clarify the impact of long-term BJ nutraceutical intervention on cellular mechanisms that govern hepatic lipid homeostasis, namely autophagy and endoplasmic reticulum (ER) stress, in a rat model of prediabetes. Two groups of male Wistar rats, 8-weeks old, were fed a prediabetes-inducing high-fat diet (HFD) and one group was fed a control diet (CD). From the timepoint where the prediabetic phenotype was achieved (week 16) until the end of the study (week 24), one of the HFD-fed groups was daily orally supplemented with 25 g/kg body weight (BW) of BJ (HFD + BJ). BW, caloric intake, glucose tolerance and insulin sensitivity were monitored throughout the study. The serum and hepatic lipid contents were quantified. Liver and interscapular brown and epidydimal white adipose tissue depots (iBAT and eWAT) were collected for histological analysis and to assess thermogenesis, ER stress and autophagy markers. The gut microbiota composition and the short-chain fatty acids (SCFAs) content were determined in colon fecal samples. BJ supplementation positively impacted glycemic control but was unable to prevent obesity and adiposity. BJ-treated animals presented a reduction in fecal SCFAs, increased markers of arrested iBAT thermogenesis and energy expenditure, together with an aggravation of HFD-induced lipotoxicity and hepatic steatosis, which were accompanied by the inhibition of autophagy and ER stress responses in the liver. In conclusion, despite the improvement of glucose tolerance, BJ supplementation promoted a major impact on lipid management mechanisms at liver and AT levels in prediabetic animals, which might affect disease course.
publishDate 2024
dc.date.none.fl_str_mv 2024-02-16T12:22:56Z
2024-02-13
2024-02-13T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/43998
url http://hdl.handle.net/10400.14/43998
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2072-6643
10.3390/nu16040513
85185944396
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