Short- and Long-Term Exposure to Heavy Metals Induced Oxidative Stress Response in Pseudokirchneriella subcapitata
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/9170 |
Resumo: | Algal cells can be exposed to toxicants for a short-term due to accidental discharges or, more commonly, for a long-term. The present work aimed to assess the ability of Cd, Cr, Cu, and Zn to induce accumulation of reactive oxygen species (ROS) in the alga Pseudokirchneriella subcapitata after a short (6 h) or a long (72 h) exposure time. The relationship between the ROS induction and the content of reduced glutathione (GSH) was also examined. For this purpose, three nominal concentrations of each metal were used corresponding approximately to 72 h-EC10 and 72 h-EC50 values and a high concentration (>72 h-EC90 values). Intracellular ROS accumulation and GSH content were evaluated using a fluorescent-based approach. A long-term (chronic) exposure of algal cells to Cd, Cu, and Zn, at the highest concentrations tested, induced an increase of intracellular ROS and GSH content. The increase of GSH content might be a form of algal cells to redress the imbalance caused by the oxidative stress. However, the increase of GSH was not enough to protect the algal cells against the long-term exposure to oxidative stress. The exposure of algal cells to low or intermediate metals concentrations induced a modification of GSH content; however, no increase of ROS production was detected, which indicates that the toxic symptoms exhibited by algal cells, under these conditions, cannot be attributed to intracellular ROS accumulation. |
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Short- and Long-Term Exposure to Heavy Metals Induced Oxidative Stress Response in Pseudokirchneriella subcapitata20,70-Dichlorodihydrofluorescein diacetate (H2DCFDA)Monochlorobimane (mBCl)Reactive oxygen species (ROS)Reduced glutathione (GSH)ToxicityAlgal cells can be exposed to toxicants for a short-term due to accidental discharges or, more commonly, for a long-term. The present work aimed to assess the ability of Cd, Cr, Cu, and Zn to induce accumulation of reactive oxygen species (ROS) in the alga Pseudokirchneriella subcapitata after a short (6 h) or a long (72 h) exposure time. The relationship between the ROS induction and the content of reduced glutathione (GSH) was also examined. For this purpose, three nominal concentrations of each metal were used corresponding approximately to 72 h-EC10 and 72 h-EC50 values and a high concentration (>72 h-EC90 values). Intracellular ROS accumulation and GSH content were evaluated using a fluorescent-based approach. A long-term (chronic) exposure of algal cells to Cd, Cu, and Zn, at the highest concentrations tested, induced an increase of intracellular ROS and GSH content. The increase of GSH content might be a form of algal cells to redress the imbalance caused by the oxidative stress. However, the increase of GSH was not enough to protect the algal cells against the long-term exposure to oxidative stress. The exposure of algal cells to low or intermediate metals concentrations induced a modification of GSH content; however, no increase of ROS production was detected, which indicates that the toxic symptoms exhibited by algal cells, under these conditions, cannot be attributed to intracellular ROS accumulation.Wiley-VCH VerlagRepositório Científico do Instituto Politécnico do PortoMachado, Manuela D.Soares, Eduardo V.20162117-01-01T00:00:00Z2016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/9170eng10.1002/clen.201600020metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:50:14Zoai:recipp.ipp.pt:10400.22/9170Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:29:43.729347Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Short- and Long-Term Exposure to Heavy Metals Induced Oxidative Stress Response in Pseudokirchneriella subcapitata |
title |
Short- and Long-Term Exposure to Heavy Metals Induced Oxidative Stress Response in Pseudokirchneriella subcapitata |
spellingShingle |
Short- and Long-Term Exposure to Heavy Metals Induced Oxidative Stress Response in Pseudokirchneriella subcapitata Machado, Manuela D. 20,70-Dichlorodihydrofluorescein diacetate (H2DCFDA) Monochlorobimane (mBCl) Reactive oxygen species (ROS) Reduced glutathione (GSH) Toxicity |
title_short |
Short- and Long-Term Exposure to Heavy Metals Induced Oxidative Stress Response in Pseudokirchneriella subcapitata |
title_full |
Short- and Long-Term Exposure to Heavy Metals Induced Oxidative Stress Response in Pseudokirchneriella subcapitata |
title_fullStr |
Short- and Long-Term Exposure to Heavy Metals Induced Oxidative Stress Response in Pseudokirchneriella subcapitata |
title_full_unstemmed |
Short- and Long-Term Exposure to Heavy Metals Induced Oxidative Stress Response in Pseudokirchneriella subcapitata |
title_sort |
Short- and Long-Term Exposure to Heavy Metals Induced Oxidative Stress Response in Pseudokirchneriella subcapitata |
author |
Machado, Manuela D. |
author_facet |
Machado, Manuela D. Soares, Eduardo V. |
author_role |
author |
author2 |
Soares, Eduardo V. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Machado, Manuela D. Soares, Eduardo V. |
dc.subject.por.fl_str_mv |
20,70-Dichlorodihydrofluorescein diacetate (H2DCFDA) Monochlorobimane (mBCl) Reactive oxygen species (ROS) Reduced glutathione (GSH) Toxicity |
topic |
20,70-Dichlorodihydrofluorescein diacetate (H2DCFDA) Monochlorobimane (mBCl) Reactive oxygen species (ROS) Reduced glutathione (GSH) Toxicity |
description |
Algal cells can be exposed to toxicants for a short-term due to accidental discharges or, more commonly, for a long-term. The present work aimed to assess the ability of Cd, Cr, Cu, and Zn to induce accumulation of reactive oxygen species (ROS) in the alga Pseudokirchneriella subcapitata after a short (6 h) or a long (72 h) exposure time. The relationship between the ROS induction and the content of reduced glutathione (GSH) was also examined. For this purpose, three nominal concentrations of each metal were used corresponding approximately to 72 h-EC10 and 72 h-EC50 values and a high concentration (>72 h-EC90 values). Intracellular ROS accumulation and GSH content were evaluated using a fluorescent-based approach. A long-term (chronic) exposure of algal cells to Cd, Cu, and Zn, at the highest concentrations tested, induced an increase of intracellular ROS and GSH content. The increase of GSH content might be a form of algal cells to redress the imbalance caused by the oxidative stress. However, the increase of GSH was not enough to protect the algal cells against the long-term exposure to oxidative stress. The exposure of algal cells to low or intermediate metals concentrations induced a modification of GSH content; however, no increase of ROS production was detected, which indicates that the toxic symptoms exhibited by algal cells, under these conditions, cannot be attributed to intracellular ROS accumulation. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z 2117-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/9170 |
url |
http://hdl.handle.net/10400.22/9170 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1002/clen.201600020 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley-VCH Verlag |
publisher.none.fl_str_mv |
Wiley-VCH Verlag |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131392807796736 |