Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/31849 |
Resumo: | This study aimed to evaluate the effect of the compound 1,4-dihydroxy-2E,6E,12E-trien-5-one-casbane (CD1CN), a casbane diterpene isolated from Croton nepetaefolius stalks, on the biofilm formation or preformed biofilms of bacteria and yeasts. Minimum inhibitory concentration results showed that CD1CN inhibited the growth of single cultures of Staphylococcus aureus and Candida albicans at 125 and 500 g/mL, respectively, as well as dual cultures of S. aureus with C. albicans or Candida glabrata at 500 and 250 g/mL, respectively. In general, CD1CN reduced biofilm biomass when applied to preformed biofilms or when applied during the biofilm formation of single and dual cultures in concentrations ranging from 31.25 to 250 g/mL, depending on the culture. CD1CN was more effective in reducing the cfu of S. aureus in single and dual biofilms (62.5–250 g/mL) than that of Pseudomonas aeruginosa, although this reduction was also significant. For yeasts, CD1CN was generally more effective in reducing C. glabrata cfu in single or dual cultures when compared to C. albicans. SEM images of the dual-species biofilms confirmed these results. In conclusion, CD1CN could be an effective alternative to conventional antimicrobial agents against infectious biofilms, in particular those attributed to mixed cultures. |
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Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida speciesBiofilmPolymicrobial infectionsBacteriaCandidaCasbane diterpeneScience & TechnologyThis study aimed to evaluate the effect of the compound 1,4-dihydroxy-2E,6E,12E-trien-5-one-casbane (CD1CN), a casbane diterpene isolated from Croton nepetaefolius stalks, on the biofilm formation or preformed biofilms of bacteria and yeasts. Minimum inhibitory concentration results showed that CD1CN inhibited the growth of single cultures of Staphylococcus aureus and Candida albicans at 125 and 500 g/mL, respectively, as well as dual cultures of S. aureus with C. albicans or Candida glabrata at 500 and 250 g/mL, respectively. In general, CD1CN reduced biofilm biomass when applied to preformed biofilms or when applied during the biofilm formation of single and dual cultures in concentrations ranging from 31.25 to 250 g/mL, depending on the culture. CD1CN was more effective in reducing the cfu of S. aureus in single and dual biofilms (62.5–250 g/mL) than that of Pseudomonas aeruginosa, although this reduction was also significant. For yeasts, CD1CN was generally more effective in reducing C. glabrata cfu in single or dual cultures when compared to C. albicans. SEM images of the dual-species biofilms confirmed these results. In conclusion, CD1CN could be an effective alternative to conventional antimicrobial agents against infectious biofilms, in particular those attributed to mixed cultures.This study was supported by the Coordenacao de Aperfeicomento de Pessoal de Nivel Superior (CAPES - Brazil) through the BEX NT 2052/11NT3 project managed by the Instituto de Biotecnologia e Bioengenharia-Centro de Engenharia Biologica (IBB-CEB) and Fundacao para a Ciencia e a Tecnologia (FCT - Portugal); by the European Community fund FEDER through the COMPETE Program under the auspices of Project PTDC/SAU-ESA/646091/2006/FCOMP-01-0124-FEDER-007480; and by Fundacao Cearense de Apoio ao Desenvolvimento Cientifico e Tecnologico (Funcap - Brazil) through the BPI-0067-00068.01.00/12 project. BSC and EHT are senior researchers of CNPq. Mr. David Martin helped with the English editing of the manuscript that was also revised by AJE (American Journal Experts).Elsevier B.V.Universidade do MinhoVasconcelos, Mayron AlvesArruda, Francisco Vassiliepe SousaSantos, Hélcio Silva dosRodrigues, Adriano S.Bandeira, Paulo NogueiraAlbuquerque, Maria Rose Jane RibeiroCavada, Benildo SousaTeixeira, Edson HolandaHenriques, MarianaPereira, Maria Olívia20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31849engVasconcelos, M. A.; Arruda, F. V. S.; Santos, Hélcio S.; Rodrigues, Adriano S.; Bandeira, Paulo N.; Albuquerque, Maria R. J. R.; Cavada, Benildo S.; Teixeira, Edson H.; Henriques, Mariana; Pereira, M. O., Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species. Industrial Crops and Products, 61, 499-509, 20140926-66900926-669010.1016/j.indcrop.2014.07.027info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:31:10Zoai:repositorium.sdum.uminho.pt:1822/31849Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:26:24.550667Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species |
title |
Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species |
spellingShingle |
Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species Vasconcelos, Mayron Alves Biofilm Polymicrobial infections Bacteria Candida Casbane diterpene Science & Technology |
title_short |
Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species |
title_full |
Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species |
title_fullStr |
Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species |
title_full_unstemmed |
Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species |
title_sort |
Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species |
author |
Vasconcelos, Mayron Alves |
author_facet |
Vasconcelos, Mayron Alves Arruda, Francisco Vassiliepe Sousa Santos, Hélcio Silva dos Rodrigues, Adriano S. Bandeira, Paulo Nogueira Albuquerque, Maria Rose Jane Ribeiro Cavada, Benildo Sousa Teixeira, Edson Holanda Henriques, Mariana Pereira, Maria Olívia |
author_role |
author |
author2 |
Arruda, Francisco Vassiliepe Sousa Santos, Hélcio Silva dos Rodrigues, Adriano S. Bandeira, Paulo Nogueira Albuquerque, Maria Rose Jane Ribeiro Cavada, Benildo Sousa Teixeira, Edson Holanda Henriques, Mariana Pereira, Maria Olívia |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Vasconcelos, Mayron Alves Arruda, Francisco Vassiliepe Sousa Santos, Hélcio Silva dos Rodrigues, Adriano S. Bandeira, Paulo Nogueira Albuquerque, Maria Rose Jane Ribeiro Cavada, Benildo Sousa Teixeira, Edson Holanda Henriques, Mariana Pereira, Maria Olívia |
dc.subject.por.fl_str_mv |
Biofilm Polymicrobial infections Bacteria Candida Casbane diterpene Science & Technology |
topic |
Biofilm Polymicrobial infections Bacteria Candida Casbane diterpene Science & Technology |
description |
This study aimed to evaluate the effect of the compound 1,4-dihydroxy-2E,6E,12E-trien-5-one-casbane (CD1CN), a casbane diterpene isolated from Croton nepetaefolius stalks, on the biofilm formation or preformed biofilms of bacteria and yeasts. Minimum inhibitory concentration results showed that CD1CN inhibited the growth of single cultures of Staphylococcus aureus and Candida albicans at 125 and 500 g/mL, respectively, as well as dual cultures of S. aureus with C. albicans or Candida glabrata at 500 and 250 g/mL, respectively. In general, CD1CN reduced biofilm biomass when applied to preformed biofilms or when applied during the biofilm formation of single and dual cultures in concentrations ranging from 31.25 to 250 g/mL, depending on the culture. CD1CN was more effective in reducing the cfu of S. aureus in single and dual biofilms (62.5–250 g/mL) than that of Pseudomonas aeruginosa, although this reduction was also significant. For yeasts, CD1CN was generally more effective in reducing C. glabrata cfu in single or dual cultures when compared to C. albicans. SEM images of the dual-species biofilms confirmed these results. In conclusion, CD1CN could be an effective alternative to conventional antimicrobial agents against infectious biofilms, in particular those attributed to mixed cultures. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/31849 |
url |
http://hdl.handle.net/1822/31849 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Vasconcelos, M. A.; Arruda, F. V. S.; Santos, Hélcio S.; Rodrigues, Adriano S.; Bandeira, Paulo N.; Albuquerque, Maria R. J. R.; Cavada, Benildo S.; Teixeira, Edson H.; Henriques, Mariana; Pereira, M. O., Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species. Industrial Crops and Products, 61, 499-509, 2014 0926-6690 0926-6690 10.1016/j.indcrop.2014.07.027 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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