Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species

Detalhes bibliográficos
Autor(a) principal: Vasconcelos, Mayron Alves
Data de Publicação: 2014
Outros Autores: Arruda, Francisco Vassiliepe Sousa, Santos, Hélcio Silva dos, Rodrigues, Adriano S., Bandeira, Paulo Nogueira, Albuquerque, Maria Rose Jane Ribeiro, Cavada, Benildo Sousa, Teixeira, Edson Holanda, Henriques, Mariana, Pereira, Maria Olívia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/31849
Resumo: This study aimed to evaluate the effect of the compound 1,4-dihydroxy-2E,6E,12E-trien-5-one-casbane (CD1CN), a casbane diterpene isolated from Croton nepetaefolius stalks, on the biofilm formation or preformed biofilms of bacteria and yeasts. Minimum inhibitory concentration results showed that CD1CN inhibited the growth of single cultures of Staphylococcus aureus and Candida albicans at 125 and 500 g/mL, respectively, as well as dual cultures of S. aureus with C. albicans or Candida glabrata at 500 and 250 g/mL, respectively. In general, CD1CN reduced biofilm biomass when applied to preformed biofilms or when applied during the biofilm formation of single and dual cultures in concentrations ranging from 31.25 to 250 g/mL, depending on the culture. CD1CN was more effective in reducing the cfu of S. aureus in single and dual biofilms (62.5–250 g/mL) than that of Pseudomonas aeruginosa, although this reduction was also significant. For yeasts, CD1CN was generally more effective in reducing C. glabrata cfu in single or dual cultures when compared to C. albicans. SEM images of the dual-species biofilms confirmed these results. In conclusion, CD1CN could be an effective alternative to conventional antimicrobial agents against infectious biofilms, in particular those attributed to mixed cultures.
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spelling Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida speciesBiofilmPolymicrobial infectionsBacteriaCandidaCasbane diterpeneScience & TechnologyThis study aimed to evaluate the effect of the compound 1,4-dihydroxy-2E,6E,12E-trien-5-one-casbane (CD1CN), a casbane diterpene isolated from Croton nepetaefolius stalks, on the biofilm formation or preformed biofilms of bacteria and yeasts. Minimum inhibitory concentration results showed that CD1CN inhibited the growth of single cultures of Staphylococcus aureus and Candida albicans at 125 and 500 g/mL, respectively, as well as dual cultures of S. aureus with C. albicans or Candida glabrata at 500 and 250 g/mL, respectively. In general, CD1CN reduced biofilm biomass when applied to preformed biofilms or when applied during the biofilm formation of single and dual cultures in concentrations ranging from 31.25 to 250 g/mL, depending on the culture. CD1CN was more effective in reducing the cfu of S. aureus in single and dual biofilms (62.5–250 g/mL) than that of Pseudomonas aeruginosa, although this reduction was also significant. For yeasts, CD1CN was generally more effective in reducing C. glabrata cfu in single or dual cultures when compared to C. albicans. SEM images of the dual-species biofilms confirmed these results. In conclusion, CD1CN could be an effective alternative to conventional antimicrobial agents against infectious biofilms, in particular those attributed to mixed cultures.This study was supported by the Coordenacao de Aperfeicomento de Pessoal de Nivel Superior (CAPES - Brazil) through the BEX NT 2052/11NT3 project managed by the Instituto de Biotecnologia e Bioengenharia-Centro de Engenharia Biologica (IBB-CEB) and Fundacao para a Ciencia e a Tecnologia (FCT - Portugal); by the European Community fund FEDER through the COMPETE Program under the auspices of Project PTDC/SAU-ESA/646091/2006/FCOMP-01-0124-FEDER-007480; and by Fundacao Cearense de Apoio ao Desenvolvimento Cientifico e Tecnologico (Funcap - Brazil) through the BPI-0067-00068.01.00/12 project. BSC and EHT are senior researchers of CNPq. Mr. David Martin helped with the English editing of the manuscript that was also revised by AJE (American Journal Experts).Elsevier B.V.Universidade do MinhoVasconcelos, Mayron AlvesArruda, Francisco Vassiliepe SousaSantos, Hélcio Silva dosRodrigues, Adriano S.Bandeira, Paulo NogueiraAlbuquerque, Maria Rose Jane RibeiroCavada, Benildo SousaTeixeira, Edson HolandaHenriques, MarianaPereira, Maria Olívia20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31849engVasconcelos, M. A.; Arruda, F. V. S.; Santos, Hélcio S.; Rodrigues, Adriano S.; Bandeira, Paulo N.; Albuquerque, Maria R. J. R.; Cavada, Benildo S.; Teixeira, Edson H.; Henriques, Mariana; Pereira, M. O., Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species. Industrial Crops and Products, 61, 499-509, 20140926-66900926-669010.1016/j.indcrop.2014.07.027info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:31:10Zoai:repositorium.sdum.uminho.pt:1822/31849Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:26:24.550667Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species
title Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species
spellingShingle Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species
Vasconcelos, Mayron Alves
Biofilm
Polymicrobial infections
Bacteria
Candida
Casbane diterpene
Science & Technology
title_short Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species
title_full Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species
title_fullStr Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species
title_full_unstemmed Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species
title_sort Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species
author Vasconcelos, Mayron Alves
author_facet Vasconcelos, Mayron Alves
Arruda, Francisco Vassiliepe Sousa
Santos, Hélcio Silva dos
Rodrigues, Adriano S.
Bandeira, Paulo Nogueira
Albuquerque, Maria Rose Jane Ribeiro
Cavada, Benildo Sousa
Teixeira, Edson Holanda
Henriques, Mariana
Pereira, Maria Olívia
author_role author
author2 Arruda, Francisco Vassiliepe Sousa
Santos, Hélcio Silva dos
Rodrigues, Adriano S.
Bandeira, Paulo Nogueira
Albuquerque, Maria Rose Jane Ribeiro
Cavada, Benildo Sousa
Teixeira, Edson Holanda
Henriques, Mariana
Pereira, Maria Olívia
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Vasconcelos, Mayron Alves
Arruda, Francisco Vassiliepe Sousa
Santos, Hélcio Silva dos
Rodrigues, Adriano S.
Bandeira, Paulo Nogueira
Albuquerque, Maria Rose Jane Ribeiro
Cavada, Benildo Sousa
Teixeira, Edson Holanda
Henriques, Mariana
Pereira, Maria Olívia
dc.subject.por.fl_str_mv Biofilm
Polymicrobial infections
Bacteria
Candida
Casbane diterpene
Science & Technology
topic Biofilm
Polymicrobial infections
Bacteria
Candida
Casbane diterpene
Science & Technology
description This study aimed to evaluate the effect of the compound 1,4-dihydroxy-2E,6E,12E-trien-5-one-casbane (CD1CN), a casbane diterpene isolated from Croton nepetaefolius stalks, on the biofilm formation or preformed biofilms of bacteria and yeasts. Minimum inhibitory concentration results showed that CD1CN inhibited the growth of single cultures of Staphylococcus aureus and Candida albicans at 125 and 500 g/mL, respectively, as well as dual cultures of S. aureus with C. albicans or Candida glabrata at 500 and 250 g/mL, respectively. In general, CD1CN reduced biofilm biomass when applied to preformed biofilms or when applied during the biofilm formation of single and dual cultures in concentrations ranging from 31.25 to 250 g/mL, depending on the culture. CD1CN was more effective in reducing the cfu of S. aureus in single and dual biofilms (62.5–250 g/mL) than that of Pseudomonas aeruginosa, although this reduction was also significant. For yeasts, CD1CN was generally more effective in reducing C. glabrata cfu in single or dual cultures when compared to C. albicans. SEM images of the dual-species biofilms confirmed these results. In conclusion, CD1CN could be an effective alternative to conventional antimicrobial agents against infectious biofilms, in particular those attributed to mixed cultures.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/31849
url http://hdl.handle.net/1822/31849
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Vasconcelos, M. A.; Arruda, F. V. S.; Santos, Hélcio S.; Rodrigues, Adriano S.; Bandeira, Paulo N.; Albuquerque, Maria R. J. R.; Cavada, Benildo S.; Teixeira, Edson H.; Henriques, Mariana; Pereira, M. O., Effect of a casbane diterpene isolated from Croton nepetaefolius on the prevention and control of biofilms formed by bacteria and Candida species. Industrial Crops and Products, 61, 499-509, 2014
0926-6690
0926-6690
10.1016/j.indcrop.2014.07.027
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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