Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/31433 |
Resumo: | This study aimed to evaluate the abilities of plant and algae lectins to inhibit planktonic growth and biofilm formation in bacteria and yeasts. Initially, ten lectins were tested on Staphylococcus epidermidis, Staphylococcus aureus, Klebsiella oxytoca, Pseudomonas aeruginosa, Candida albicans, and C. tropicalis at concentrations of 31.25 to 250 μg/mL. The lectins from Cratylia floribunda (CFL), Vatairea macrocarpa (VML), Bauhinia bauhinioides (BBL), Bryothamnion seaforthii (BSL), and Hypnea musciformis (HML) showed activities against at least one microorganism. Biofilm formation in the presence of the lectins was also evaluated; after 24 h of incubation with the lectins, the biofilms were analyzed by quantifying the biomass (by crystal violet staining) and by enumerating the viable cells (colony-forming units). The lectins reduced the biofilm biomass and/or the number of viable cells to differing degrees depending on the microorganism tested, demonstrating the different characteristics of the lectins. These findings indicate that the lectins tested in this study may be natural alternative antimicrobial agents; however, further studies are required to better elucidate the functional use of these proteins. |
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Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeastsScience & TechnologyThis study aimed to evaluate the abilities of plant and algae lectins to inhibit planktonic growth and biofilm formation in bacteria and yeasts. Initially, ten lectins were tested on Staphylococcus epidermidis, Staphylococcus aureus, Klebsiella oxytoca, Pseudomonas aeruginosa, Candida albicans, and C. tropicalis at concentrations of 31.25 to 250 μg/mL. The lectins from Cratylia floribunda (CFL), Vatairea macrocarpa (VML), Bauhinia bauhinioides (BBL), Bryothamnion seaforthii (BSL), and Hypnea musciformis (HML) showed activities against at least one microorganism. Biofilm formation in the presence of the lectins was also evaluated; after 24 h of incubation with the lectins, the biofilms were analyzed by quantifying the biomass (by crystal violet staining) and by enumerating the viable cells (colony-forming units). The lectins reduced the biofilm biomass and/or the number of viable cells to differing degrees depending on the microorganism tested, demonstrating the different characteristics of the lectins. These findings indicate that the lectins tested in this study may be natural alternative antimicrobial agents; however, further studies are required to better elucidate the functional use of these proteins.This study was supported by the CAPES (Brazil) under the BEX NT 2052/11NT3 Project, by the IBB-CEB and FCT (Portugal), by the European Community Fund FEDER, and by the COMPETE Program under the auspices of the PTDC/SAU-ESA/646091/2006/FCOMP-01-0124-FEDER-007480 Project. Kyria Santiago Nascimento, Alexandre Holanda Sampaio, Benildo Sousa Cavada, and Edson Holanda Teixeira are Senior Fellows of CNPq. Mr. David Martin helped with the English editing of the paper that was also revised by AJE (American Journal Experts).Hindawi Publishing CorporationUniversidade do MinhoVasconcelos, Mayron AlvesArruda, Francisco Vassiliepe SousaCarneiro, Victor AlvesSilva, H. C.Nascimento, Kyria SantiagoSampaio, A. H.Cavada, Benildo SousaTeixeira, Edson HolandaHenriques, MarianaPereira, Maria Olívia20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31433engVasconcelos, M. A.; Arruda, F. V. S.; Carneiro, V. A.; Silva, H. C.; Nascimento, K. S.; Sampaio, A. H.; Cavada, B.; Teixeira, E. H.; Henriques, Mariana; Pereira, M. O., Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts. BioMed Research International, 2014(365272), 1-9, 20142314-61332314-614110.1155/2014/36527224982871http://www.hindawi.com/journals/bmri/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:25:49Zoai:repositorium.sdum.uminho.pt:1822/31433Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:20:07.800873Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts |
title |
Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts |
spellingShingle |
Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts Vasconcelos, Mayron Alves Science & Technology |
title_short |
Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts |
title_full |
Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts |
title_fullStr |
Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts |
title_full_unstemmed |
Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts |
title_sort |
Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts |
author |
Vasconcelos, Mayron Alves |
author_facet |
Vasconcelos, Mayron Alves Arruda, Francisco Vassiliepe Sousa Carneiro, Victor Alves Silva, H. C. Nascimento, Kyria Santiago Sampaio, A. H. Cavada, Benildo Sousa Teixeira, Edson Holanda Henriques, Mariana Pereira, Maria Olívia |
author_role |
author |
author2 |
Arruda, Francisco Vassiliepe Sousa Carneiro, Victor Alves Silva, H. C. Nascimento, Kyria Santiago Sampaio, A. H. Cavada, Benildo Sousa Teixeira, Edson Holanda Henriques, Mariana Pereira, Maria Olívia |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Vasconcelos, Mayron Alves Arruda, Francisco Vassiliepe Sousa Carneiro, Victor Alves Silva, H. C. Nascimento, Kyria Santiago Sampaio, A. H. Cavada, Benildo Sousa Teixeira, Edson Holanda Henriques, Mariana Pereira, Maria Olívia |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
This study aimed to evaluate the abilities of plant and algae lectins to inhibit planktonic growth and biofilm formation in bacteria and yeasts. Initially, ten lectins were tested on Staphylococcus epidermidis, Staphylococcus aureus, Klebsiella oxytoca, Pseudomonas aeruginosa, Candida albicans, and C. tropicalis at concentrations of 31.25 to 250 μg/mL. The lectins from Cratylia floribunda (CFL), Vatairea macrocarpa (VML), Bauhinia bauhinioides (BBL), Bryothamnion seaforthii (BSL), and Hypnea musciformis (HML) showed activities against at least one microorganism. Biofilm formation in the presence of the lectins was also evaluated; after 24 h of incubation with the lectins, the biofilms were analyzed by quantifying the biomass (by crystal violet staining) and by enumerating the viable cells (colony-forming units). The lectins reduced the biofilm biomass and/or the number of viable cells to differing degrees depending on the microorganism tested, demonstrating the different characteristics of the lectins. These findings indicate that the lectins tested in this study may be natural alternative antimicrobial agents; however, further studies are required to better elucidate the functional use of these proteins. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/31433 |
url |
http://hdl.handle.net/1822/31433 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Vasconcelos, M. A.; Arruda, F. V. S.; Carneiro, V. A.; Silva, H. C.; Nascimento, K. S.; Sampaio, A. H.; Cavada, B.; Teixeira, E. H.; Henriques, Mariana; Pereira, M. O., Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts. BioMed Research International, 2014(365272), 1-9, 2014 2314-6133 2314-6141 10.1155/2014/365272 24982871 http://www.hindawi.com/journals/bmri/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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