Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts

Detalhes bibliográficos
Autor(a) principal: Vasconcelos, Mayron Alves
Data de Publicação: 2014
Outros Autores: Arruda, Francisco Vassiliepe Sousa, Carneiro, Victor Alves, Silva, H. C., Nascimento, Kyria Santiago, Sampaio, A. H., Cavada, Benildo Sousa, Teixeira, Edson Holanda, Henriques, Mariana, Pereira, Maria Olívia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/31433
Resumo: This study aimed to evaluate the abilities of plant and algae lectins to inhibit planktonic growth and biofilm formation in bacteria and yeasts. Initially, ten lectins were tested on Staphylococcus epidermidis, Staphylococcus aureus, Klebsiella oxytoca, Pseudomonas aeruginosa, Candida albicans, and C. tropicalis at concentrations of 31.25 to 250 μg/mL. The lectins from Cratylia floribunda (CFL), Vatairea macrocarpa (VML), Bauhinia bauhinioides (BBL), Bryothamnion seaforthii (BSL), and Hypnea musciformis (HML) showed activities against at least one microorganism. Biofilm formation in the presence of the lectins was also evaluated; after 24 h of incubation with the lectins, the biofilms were analyzed by quantifying the biomass (by crystal violet staining) and by enumerating the viable cells (colony-forming units). The lectins reduced the biofilm biomass and/or the number of viable cells to differing degrees depending on the microorganism tested, demonstrating the different characteristics of the lectins. These findings indicate that the lectins tested in this study may be natural alternative antimicrobial agents; however, further studies are required to better elucidate the functional use of these proteins.
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spelling Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeastsScience & TechnologyThis study aimed to evaluate the abilities of plant and algae lectins to inhibit planktonic growth and biofilm formation in bacteria and yeasts. Initially, ten lectins were tested on Staphylococcus epidermidis, Staphylococcus aureus, Klebsiella oxytoca, Pseudomonas aeruginosa, Candida albicans, and C. tropicalis at concentrations of 31.25 to 250 μg/mL. The lectins from Cratylia floribunda (CFL), Vatairea macrocarpa (VML), Bauhinia bauhinioides (BBL), Bryothamnion seaforthii (BSL), and Hypnea musciformis (HML) showed activities against at least one microorganism. Biofilm formation in the presence of the lectins was also evaluated; after 24 h of incubation with the lectins, the biofilms were analyzed by quantifying the biomass (by crystal violet staining) and by enumerating the viable cells (colony-forming units). The lectins reduced the biofilm biomass and/or the number of viable cells to differing degrees depending on the microorganism tested, demonstrating the different characteristics of the lectins. These findings indicate that the lectins tested in this study may be natural alternative antimicrobial agents; however, further studies are required to better elucidate the functional use of these proteins.This study was supported by the CAPES (Brazil) under the BEX NT 2052/11NT3 Project, by the IBB-CEB and FCT (Portugal), by the European Community Fund FEDER, and by the COMPETE Program under the auspices of the PTDC/SAU-ESA/646091/2006/FCOMP-01-0124-FEDER-007480 Project. Kyria Santiago Nascimento, Alexandre Holanda Sampaio, Benildo Sousa Cavada, and Edson Holanda Teixeira are Senior Fellows of CNPq. Mr. David Martin helped with the English editing of the paper that was also revised by AJE (American Journal Experts).Hindawi Publishing CorporationUniversidade do MinhoVasconcelos, Mayron AlvesArruda, Francisco Vassiliepe SousaCarneiro, Victor AlvesSilva, H. C.Nascimento, Kyria SantiagoSampaio, A. H.Cavada, Benildo SousaTeixeira, Edson HolandaHenriques, MarianaPereira, Maria Olívia20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31433engVasconcelos, M. A.; Arruda, F. V. S.; Carneiro, V. A.; Silva, H. C.; Nascimento, K. S.; Sampaio, A. H.; Cavada, B.; Teixeira, E. H.; Henriques, Mariana; Pereira, M. O., Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts. BioMed Research International, 2014(365272), 1-9, 20142314-61332314-614110.1155/2014/36527224982871http://www.hindawi.com/journals/bmri/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:25:49Zoai:repositorium.sdum.uminho.pt:1822/31433Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:20:07.800873Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts
title Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts
spellingShingle Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts
Vasconcelos, Mayron Alves
Science & Technology
title_short Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts
title_full Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts
title_fullStr Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts
title_full_unstemmed Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts
title_sort Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts
author Vasconcelos, Mayron Alves
author_facet Vasconcelos, Mayron Alves
Arruda, Francisco Vassiliepe Sousa
Carneiro, Victor Alves
Silva, H. C.
Nascimento, Kyria Santiago
Sampaio, A. H.
Cavada, Benildo Sousa
Teixeira, Edson Holanda
Henriques, Mariana
Pereira, Maria Olívia
author_role author
author2 Arruda, Francisco Vassiliepe Sousa
Carneiro, Victor Alves
Silva, H. C.
Nascimento, Kyria Santiago
Sampaio, A. H.
Cavada, Benildo Sousa
Teixeira, Edson Holanda
Henriques, Mariana
Pereira, Maria Olívia
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Vasconcelos, Mayron Alves
Arruda, Francisco Vassiliepe Sousa
Carneiro, Victor Alves
Silva, H. C.
Nascimento, Kyria Santiago
Sampaio, A. H.
Cavada, Benildo Sousa
Teixeira, Edson Holanda
Henriques, Mariana
Pereira, Maria Olívia
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description This study aimed to evaluate the abilities of plant and algae lectins to inhibit planktonic growth and biofilm formation in bacteria and yeasts. Initially, ten lectins were tested on Staphylococcus epidermidis, Staphylococcus aureus, Klebsiella oxytoca, Pseudomonas aeruginosa, Candida albicans, and C. tropicalis at concentrations of 31.25 to 250 μg/mL. The lectins from Cratylia floribunda (CFL), Vatairea macrocarpa (VML), Bauhinia bauhinioides (BBL), Bryothamnion seaforthii (BSL), and Hypnea musciformis (HML) showed activities against at least one microorganism. Biofilm formation in the presence of the lectins was also evaluated; after 24 h of incubation with the lectins, the biofilms were analyzed by quantifying the biomass (by crystal violet staining) and by enumerating the viable cells (colony-forming units). The lectins reduced the biofilm biomass and/or the number of viable cells to differing degrees depending on the microorganism tested, demonstrating the different characteristics of the lectins. These findings indicate that the lectins tested in this study may be natural alternative antimicrobial agents; however, further studies are required to better elucidate the functional use of these proteins.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/31433
url http://hdl.handle.net/1822/31433
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Vasconcelos, M. A.; Arruda, F. V. S.; Carneiro, V. A.; Silva, H. C.; Nascimento, K. S.; Sampaio, A. H.; Cavada, B.; Teixeira, E. H.; Henriques, Mariana; Pereira, M. O., Effect of algae and plant lectins on planktonic growth and biofilm formation in clinically relevant bacteria and yeasts. BioMed Research International, 2014(365272), 1-9, 2014
2314-6133
2314-6141
10.1155/2014/365272
24982871
http://www.hindawi.com/journals/bmri/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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