Enzymatic degradation of 3D scaffolds of starch-poly-(epsilon-caprolactone) prepared by supercritical fluid technology

Detalhes bibliográficos
Autor(a) principal: Duarte, Ana Rita C.
Data de Publicação: 2010
Outros Autores: Mano, J. F., Reis, R. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/20545
Resumo: Starch-based polymers have been proposed for different tissue engineering applications due to their inherent properties. In this work, a polymeric blend of starch-poly-(3-caprolactone) (SPCL) was processed using supercritical fluid technology, namely, by supercritical assisted phase inversion. As SPCL is a biodegradable polymer, the matrices produced are susceptible of undergoing enzymatic degradation upon implantation in the human body. In vitro assessment of the enzymatic degradation of SPCL was carried out in different buffer solutions containing a-amylase and/or lipase. The effect of the presence of these enzymes was studied by monitoring different parameters in order to characterise both bulk and the surface of the scaffolds. As regards to bulk analysis, weight loss of the samples incubated for 1, 3, 7, 14 and 21 days was determined, further differential scanning calorimetry was carried out. The morphology of the scaffolds after these periods was analysed by micro-computed tomography (m-CT) and surface chemistry was characterised by infra-red spectroscopy and contact angle measurements. Results suggest that SPLC scaffolds undergo bulk degradation, which is typically characterised by hydrolysis of chemical bonds in the polymer chain at the centre of the matrix, resulting in a highly porous material.
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spelling Enzymatic degradation of 3D scaffolds of starch-poly-(epsilon-caprolactone) prepared by supercritical fluid technologySupercritical fluidsStarchPolycaprolactoneNatural polymersEnzymatic degradationTissue engineeringScience & TechnologyStarch-based polymers have been proposed for different tissue engineering applications due to their inherent properties. In this work, a polymeric blend of starch-poly-(3-caprolactone) (SPCL) was processed using supercritical fluid technology, namely, by supercritical assisted phase inversion. As SPCL is a biodegradable polymer, the matrices produced are susceptible of undergoing enzymatic degradation upon implantation in the human body. In vitro assessment of the enzymatic degradation of SPCL was carried out in different buffer solutions containing a-amylase and/or lipase. The effect of the presence of these enzymes was studied by monitoring different parameters in order to characterise both bulk and the surface of the scaffolds. As regards to bulk analysis, weight loss of the samples incubated for 1, 3, 7, 14 and 21 days was determined, further differential scanning calorimetry was carried out. The morphology of the scaffolds after these periods was analysed by micro-computed tomography (m-CT) and surface chemistry was characterised by infra-red spectroscopy and contact angle measurements. Results suggest that SPLC scaffolds undergo bulk degradation, which is typically characterised by hydrolysis of chemical bonds in the polymer chain at the centre of the matrix, resulting in a highly porous material.Ana Rita C. Duarte is grateful for financial support from Fundacao para a Ciencia e Tecnologia through the grant SFRH/BPD/34994/2007. The authors acknowledge Helena S. Azevedo for her help in the review of this paper.ElsevierUniversidade do MinhoDuarte, Ana Rita C.Mano, J. F.Reis, R. L.20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/20545eng0141-391010.1016/j.polymdegradstab.2010.06.020http://www.sciencedirect.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:33:47Zoai:repositorium.sdum.uminho.pt:1822/20545Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:29:22.040289Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Enzymatic degradation of 3D scaffolds of starch-poly-(epsilon-caprolactone) prepared by supercritical fluid technology
title Enzymatic degradation of 3D scaffolds of starch-poly-(epsilon-caprolactone) prepared by supercritical fluid technology
spellingShingle Enzymatic degradation of 3D scaffolds of starch-poly-(epsilon-caprolactone) prepared by supercritical fluid technology
Duarte, Ana Rita C.
Supercritical fluids
Starch
Polycaprolactone
Natural polymers
Enzymatic degradation
Tissue engineering
Science & Technology
title_short Enzymatic degradation of 3D scaffolds of starch-poly-(epsilon-caprolactone) prepared by supercritical fluid technology
title_full Enzymatic degradation of 3D scaffolds of starch-poly-(epsilon-caprolactone) prepared by supercritical fluid technology
title_fullStr Enzymatic degradation of 3D scaffolds of starch-poly-(epsilon-caprolactone) prepared by supercritical fluid technology
title_full_unstemmed Enzymatic degradation of 3D scaffolds of starch-poly-(epsilon-caprolactone) prepared by supercritical fluid technology
title_sort Enzymatic degradation of 3D scaffolds of starch-poly-(epsilon-caprolactone) prepared by supercritical fluid technology
author Duarte, Ana Rita C.
author_facet Duarte, Ana Rita C.
Mano, J. F.
Reis, R. L.
author_role author
author2 Mano, J. F.
Reis, R. L.
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Duarte, Ana Rita C.
Mano, J. F.
Reis, R. L.
dc.subject.por.fl_str_mv Supercritical fluids
Starch
Polycaprolactone
Natural polymers
Enzymatic degradation
Tissue engineering
Science & Technology
topic Supercritical fluids
Starch
Polycaprolactone
Natural polymers
Enzymatic degradation
Tissue engineering
Science & Technology
description Starch-based polymers have been proposed for different tissue engineering applications due to their inherent properties. In this work, a polymeric blend of starch-poly-(3-caprolactone) (SPCL) was processed using supercritical fluid technology, namely, by supercritical assisted phase inversion. As SPCL is a biodegradable polymer, the matrices produced are susceptible of undergoing enzymatic degradation upon implantation in the human body. In vitro assessment of the enzymatic degradation of SPCL was carried out in different buffer solutions containing a-amylase and/or lipase. The effect of the presence of these enzymes was studied by monitoring different parameters in order to characterise both bulk and the surface of the scaffolds. As regards to bulk analysis, weight loss of the samples incubated for 1, 3, 7, 14 and 21 days was determined, further differential scanning calorimetry was carried out. The morphology of the scaffolds after these periods was analysed by micro-computed tomography (m-CT) and surface chemistry was characterised by infra-red spectroscopy and contact angle measurements. Results suggest that SPLC scaffolds undergo bulk degradation, which is typically characterised by hydrolysis of chemical bonds in the polymer chain at the centre of the matrix, resulting in a highly porous material.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/20545
url http://hdl.handle.net/1822/20545
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0141-3910
10.1016/j.polymdegradstab.2010.06.020
http://www.sciencedirect.com/
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eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
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